Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GOC52_RS16150 Genome accession   NZ_CP046588
Coordinates   3528752..3530485 (-) Length   577 a.a.
NCBI ID   WP_191849881.1    Uniprot ID   -
Organism   Stenotrophomonas sp. SXG-1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3523752..3535485
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GOC52_RS16135 sucC 3523944..3525113 (-) 1170 WP_004154430.1 ADP-forming succinate--CoA ligase subunit beta -
  GOC52_RS16140 - 3525519..3527132 (+) 1614 WP_143993947.1 ATP-binding protein -
  GOC52_RS16145 pilR 3527193..3528566 (+) 1374 WP_010481514.1 sigma-54 dependent transcriptional regulator Regulator
  GOC52_RS16150 pilB 3528752..3530485 (-) 1734 WP_191849881.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GOC52_RS16155 - 3530551..3530970 (-) 420 WP_049425142.1 pilin -
  GOC52_RS16160 - 3531079..3531540 (-) 462 WP_191849882.1 pilin -
  GOC52_RS16165 pilC 3531900..3533159 (+) 1260 WP_191849884.1 type II secretion system F family protein Machinery gene
  GOC52_RS16170 - 3533168..3534031 (+) 864 WP_049447482.1 A24 family peptidase -
  GOC52_RS16175 coaE 3534043..3534654 (+) 612 WP_097049714.1 dephospho-CoA kinase -
  GOC52_RS16180 - 3534813..3535199 (-) 387 WP_191849886.1 hypothetical protein -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62351.32 Da        Isoelectric Point: 6.3005

>NTDB_id=405702 GOC52_RS16150 WP_191849881.1 3528752..3530485(-) (pilB) [Stenotrophomonas sp. SXG-1]
MNTVVTANLVGITGLARRLVQDGALDEASARDAMAKAAAARQPLPTWFAQNKLVSASQLAAANAVEFGMPLFDVSTFDTS
QNAMSLVSEELLRKHNVLPLFKRGGKLFVGTSNPTHALDEIKFHTNLVVEPILVDEDQIRRTLEQWHASHDAIGNALGGD
DEGMGNLDVAAGDEDLGASSGDSGIDAKGDDTPVVKFVNKVLVDAIRKGASDIHFEPYEDDYRVRLRIDGLLKMVARAPV
KLNQRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQKL
FHDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPD
IIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCGNCKRPANLPE
HALLAEGFTQAQLDAGIQLYEAVGCDECTEGYKGRTGIYQVMPMTDEIATIVLAGGNALQIAEAAQQIGVNDLRQSALKK
AAAGVTSLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=405702 GOC52_RS16150 WP_191849881.1 3528752..3530485(-) (pilB) [Stenotrophomonas sp. SXG-1]
ATGAACACAGTTGTCACCGCCAACCTTGTCGGCATCACCGGCTTGGCCCGTCGCTTGGTCCAGGACGGTGCCTTGGACGA
GGCCTCCGCCCGCGACGCCATGGCCAAGGCGGCGGCTGCCCGCCAGCCGCTGCCTACGTGGTTCGCGCAGAACAAGCTGG
TCAGTGCCTCGCAGCTCGCCGCCGCCAATGCAGTCGAGTTCGGCATGCCGCTGTTCGACGTTTCCACGTTCGACACCAGC
CAGAACGCGATGAGCCTGGTCAGCGAGGAGCTGCTGCGCAAGCACAACGTGCTGCCGCTGTTCAAACGCGGCGGCAAGCT
GTTCGTGGGTACCAGCAACCCGACCCATGCGCTGGACGAGATCAAGTTCCACACCAATCTGGTGGTGGAACCGATCCTGG
TCGATGAGGACCAGATCCGCCGCACGCTTGAGCAGTGGCACGCCAGCCACGATGCCATCGGAAACGCACTGGGTGGCGAT
GACGAGGGCATGGGCAACCTGGATGTCGCGGCTGGTGACGAAGATCTCGGCGCCAGCAGCGGCGACTCTGGCATCGACGC
CAAGGGTGACGACACCCCGGTGGTGAAGTTCGTCAACAAGGTACTGGTCGATGCGATCCGCAAGGGCGCATCGGATATCC
ACTTCGAGCCCTACGAAGACGATTACCGCGTGCGCCTGCGCATCGACGGCCTGCTGAAGATGGTGGCGCGCGCGCCGGTG
AAGTTGAACCAGCGCATTGCCGCGCGTTTGAAGGTGATGGCGCAGCTGGACATCGCCGAGAAGCGCGTGCCGCAGGACGG
GCGCATCAAGCTGAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTCAGCACGCTGCCGACCCTGTTCGGTGAAAAAG
TGGTGCTGCGTATCCTCGATGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGCTACGAACCAGACCAGCAGAAGCTG
TTCCACGACGCCATCCACAAGCCGTATGGCATGGTGCTGGTGACCGGCCCGACCGGTTCGGGCAAGACCGTGTCGCTGTA
TACCGCGCTGGGCATCCTCAACGACGAGACGCGCAACATCTCCACTGCCGAAGACCCGGTGGAAATCCGCTTGCCCGGTG
TCAACCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCGGCGCTGCGCTCGTTCCTGCGACAGGATCCGGAC
ATCATCATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACCGGCCACATGGTGCT
GTCCACGCTGCATACCAACGATGCGCCGCAGACCATCGCGCGCCTGATGAACATGGGTATCGCCCCGTACAACATCACCA
GCTCGGTGACGCTGGTGATCGCGCAGCGCCTGGCACGCCGGTTGTGCGGCAACTGCAAGCGTCCGGCCAACCTGCCGGAG
CATGCGCTGCTGGCCGAGGGCTTCACCCAGGCGCAGCTGGATGCGGGCATCCAGCTGTACGAGGCGGTGGGCTGCGATGA
GTGCACCGAAGGCTACAAGGGCCGAACCGGTATCTACCAGGTGATGCCGATGACCGATGAGATCGCCACGATCGTGCTGG
CCGGTGGCAATGCGCTGCAGATTGCCGAGGCGGCGCAGCAGATCGGGGTGAATGACCTGCGGCAGTCGGCGTTGAAGAAG
GCGGCTGCGGGCGTGACCAGCCTGGCCGAGATCAATCGCGTGACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(200-467)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.294

99.133

0.558
  pilB Acinetobacter baylyi ADP1

56.063

98.614

0.553
  pilB Legionella pneumophila strain ERS1305867

51.579

98.787

0.51
  pilB Vibrio cholerae strain A1552

48.606

99.48

0.484
  pilF Neisseria gonorrhoeae MS11

48.772

98.787

0.482
  pilB Vibrio parahaemolyticus RIMD 2210633

45.804

99.133

0.454
  pilB Vibrio campbellii strain DS40M4

45.518

98.614

0.449
  pilF Thermus thermophilus HB27

37.123

97.574

0.362

Multiple sequence alignment