Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYB   Type   Machinery gene
Locus tag   GE024_RS00845 Genome accession   NZ_CP046521
Coordinates   139982..141016 (+) Length   344 a.a.
NCBI ID   WP_223824829.1    Uniprot ID   -
Organism   Streptococcus canis strain HL_100     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 134982..146016
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GE024_RS00835 (GE024_00825) - 138656..139021 (+) 366 WP_003046179.1 DUF1033 family protein -
  GE024_RS00840 (GE024_00830) comYA 139108..140049 (+) 942 WP_003046182.1 competence type IV pilus ATPase ComGA Machinery gene
  GE024_RS00845 (GE024_00835) comYB 139982..141016 (+) 1035 WP_223824829.1 competence type IV pilus assembly protein ComGB Machinery gene
  GE024_RS00850 (GE024_00840) comGC/cglC 141019..141345 (+) 327 WP_003046186.1 competence type IV pilus major pilin ComGC Machinery gene
  GE024_RS00855 (GE024_00845) comGD 141320..141748 (+) 429 WP_039994748.1 competence type IV pilus minor pilin ComGD -
  GE024_RS00860 (GE024_00850) comGE 141726..142001 (+) 276 WP_003046190.1 competence type IV pilus minor pilin ComGE -
  GE024_RS00865 (GE024_00855) comGF 141982..142422 (+) 441 WP_003046192.1 competence type IV pilus minor pilin ComGF -
  GE024_RS00870 (GE024_00860) comGG 142400..142780 (+) 381 WP_003046193.1 competence type IV pilus minor pilin ComGG -
  GE024_RS00875 (GE024_00865) comYH 142830..143783 (+) 954 WP_003046195.1 class I SAM-dependent methyltransferase Machinery gene
  GE024_RS00880 (GE024_00870) - 143842..145038 (+) 1197 WP_093998734.1 acetate kinase -
  GE024_RS00885 (GE024_00875) - 145330..145536 (+) 207 WP_003046200.1 helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 39241.54 Da        Isoelectric Point: 9.1998

>NTDB_id=404832 GE024_RS00845 WP_223824829.1 139982..141016(+) (comYB) [Streptococcus canis strain HL_100]
MISYLQQDISVRNKQGLKKLSSKEQYKLIQLLTNLLSAGFQLTDIIAFLKKSQLVKLQYVLSMEDSLLKGRGLADMLAGL
GFSDAILTQISLADGHGDIKKTLFNIQNYLKQMTQLKRKTIEVITYPIMLLLFLFVIMLGLRHYLVPQLEETNQITDFLN
HFPTYFLGFGLALVLLLGVGWLRWRSQSRLNCLSHLSTFPFLGKLLQQYLTAYYAREWGTLIGQGLELPMILDIMATEKS
SLMQELAEDIRASLLAGQAFPTKVATYPFFKKELSLMIEYGEIKSKLGQELEIYAQESWARFFSQLHQATQFIQPVIFLM
IALIIVMIYAAILLPIYQDMGGLI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=404832 GE024_RS00845 WP_223824829.1 139982..141016(+) (comYB) [Streptococcus canis strain HL_100]
TTGATCAGTTACTTGCAGCAGGACATCTCAGTCCGAAACAAGCAAGGCTTGAAAAAATTGTCCAGTAAAGAGCAATATAA
GCTTATCCAGCTGTTGACTAATCTTCTATCAGCAGGATTTCAGTTGACAGATATTATTGCTTTTTTAAAGAAAAGTCAGC
TTGTGAAACTCCAGTATGTTCTTAGTATGGAAGATTCCCTTTTAAAGGGACGAGGCTTAGCAGATATGTTAGCTGGTCTG
GGATTTTCGGATGCCATCTTGACTCAGATTAGCCTAGCAGATGGGCACGGAGATATTAAGAAGACGCTGTTTAACATTCA
GAATTACCTGAAGCAAATGACCCAGCTCAAGCGAAAGACCATTGAAGTAATAACCTATCCAATAATGCTGTTGCTTTTTC
TCTTTGTCATCATGCTAGGATTGAGACACTATTTAGTGCCCCAATTAGAAGAGACAAACCAAATAACTGACTTTCTCAAT
CATTTTCCGACCTACTTTTTGGGTTTTGGTTTGGCTCTTGTGTTACTTTTGGGAGTGGGGTGGCTGAGATGGCGGTCTCA
AAGCCGTTTAAACTGTTTAAGCCACCTAAGCACCTTTCCCTTTCTGGGAAAACTATTACAACAATACCTAACCGCCTATT
ATGCAAGGGAATGGGGCACTTTGATAGGGCAGGGTCTTGAGTTACCGATGATTCTAGACATTATGGCAACCGAGAAATCC
TCACTGATGCAAGAGTTGGCTGAGGATATTAGGGCTAGTTTACTGGCAGGACAGGCTTTTCCTACCAAAGTAGCCACTTA
CCCTTTCTTTAAAAAAGAATTAAGTTTGATGATTGAATATGGAGAAATCAAATCCAAACTAGGTCAGGAGCTTGAAATCT
ATGCTCAAGAAAGTTGGGCTCGCTTTTTCAGCCAGCTTCATCAAGCAACGCAGTTTATTCAACCAGTTATTTTTTTAATG
ATTGCCTTAATAATCGTCATGATTTATGCGGCAATCTTATTACCAATTTATCAAGATATGGGAGGCCTTATTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(215-335)

Type II secretion system (T2SS), protein F (T2SSF)

(29-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYB Streptococcus mutans UA140

53.097

98.547

0.523
  comYB Streptococcus mutans UA159

53.097

98.547

0.523
  comYB Streptococcus gordonii str. Challis substr. CH1

50

98.837

0.494
  comGB/cglB Streptococcus mitis NCTC 12261

46.588

97.965

0.456
  comGB/cglB Streptococcus mitis SK321

46.847

96.802

0.453
  comGB/cglB Streptococcus pneumoniae Rx1

44.745

96.802

0.433
  comGB/cglB Streptococcus pneumoniae D39

44.745

96.802

0.433
  comGB/cglB Streptococcus pneumoniae R6

44.745

96.802

0.433
  comGB/cglB Streptococcus pneumoniae TIGR4

44.745

96.802

0.433
  comGB Lactococcus lactis subsp. cremoris KW2

43.195

98.256

0.424

Multiple sequence alignment