Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HUU85_RS15055 Genome accession   NZ_CP058302
Coordinates   3171694..3173079 (-) Length   461 a.a.
NCBI ID   WP_126667029.1    Uniprot ID   -
Organism   Escherichia coli strain AH65     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3166694..3178079
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUU85_RS15020 (HUU85_15045) mutT 3167024..3167413 (+) 390 WP_000736007.1 8-oxo-dGTP diphosphatase MutT -
  HUU85_RS15025 (HUU85_15050) yacG 3167629..3167826 (-) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  HUU85_RS15030 (HUU85_15055) zapD 3167836..3168579 (-) 744 WP_001194734.1 cell division protein ZapD -
  HUU85_RS15035 (HUU85_15060) coaE 3168579..3169199 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  HUU85_RS15040 (HUU85_15065) - 3169224..3169268 (+) 45 WP_120795372.1 protein YacM -
  HUU85_RS15045 (HUU85_15070) guaC 3169424..3170467 (+) 1044 WP_001217338.1 GMP reductase -
  HUU85_RS15050 (HUU85_15075) hofC 3170502..3171704 (-) 1203 WP_000157266.1 protein transport protein HofC -
  HUU85_RS15055 (HUU85_15080) pilB 3171694..3173079 (-) 1386 WP_126667029.1 type II secretion system protein GspE Machinery gene
  HUU85_RS15060 (HUU85_15085) pilA 3173089..3173529 (-) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  HUU85_RS15065 (HUU85_15090) nadC 3173732..3174625 (-) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HUU85_RS15070 (HUU85_15095) ampD 3174713..3175264 (+) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HUU85_RS15075 (HUU85_15100) ampE 3175261..3176115 (+) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  HUU85_RS15080 (HUU85_15105) aroP 3176158..3177531 (-) 1374 WP_000969915.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50629.36 Da        Isoelectric Point: 6.6612

>NTDB_id=404641 HUU85_RS15055 WP_126667029.1 3171694..3173079(-) (pilB) [Escherichia coli strain AH65]
MNIPQLTALCLRYHGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLPCRGGEKVVLRLLQQVGQALDVNTLGMQPLQLADFAHALQQPQGLVLVTGPTGSGKTVTLYSALQKLN
TADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TCETLVRLQQMGIARWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGFYGRTALFE
VLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=404641 HUU85_RS15055 WP_126667029.1 3171694..3173079(-) (pilB) [Escherichia coli strain AH65]
ATGAATATTCCACAGCTCACTGCCCTGTGTCTGCGTTATCATGGAGTCTTGCTGGATGCCAGCGAAGAGGTGGTTCATGT
TGCGGTAGTCGATGCACCTTCGCATGAGCTACTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTGTTCAGGAGAAGCATCAGCCC
AAAGCAGAGTTGCTAACTCGAACGTTACAATCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGATGTTTCACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGAAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTACCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGGGTCAGGCACTGGATGTCAACACGCTTGGAATGCAGCCGTTACAACTGGCGGACTTTGCTCATGCCTTGCAACAAC
CACAGGGACTGGTGCTGGTAACTGGCCCTACCGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAAAGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCAGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATCC
GCGTGCCGGACTCACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGATCCTGACGTCATCATGATCGGAGAGATCC
GCGATGGCGAAACAGCAGAGATCGCTATTAAAGCGGCGCAAACTGGTCACCTGGTGTTGTCTACCCTACACACTAATTCC
ACCTGCGAAACGCTGGTACGTTTACAGCAAATGGGGATCGCCCGCTGGATGCTATCATCGGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGCAAACTTTGCCCACATTGTCGCCGGCAGCAAGGGGAGCCCATCCACATTCCAGACAATGTATGGC
CATCGCCGCTGCCCCACTGGCAGGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGTACGGCCTTATTTGAA
GTTCTGCCCATAACGCCGGTCATTCGTCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACGCACGCACGACA
GGCGGGTATGCGTACGCTTTTTGAAAACGGCTGCCTGGCCGTAGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TACTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)

Type II/IV secretion system protein (T2SSE)

(88-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

42.236

100

0.443
  pilB Legionella pneumophila strain ERS1305867

50.26

83.297

0.419
  pilB Glaesserella parasuis strain SC1401

41.469

100

0.416
  pilB Acinetobacter baylyi ADP1

39.744

100

0.403
  pilB Vibrio cholerae strain A1552

46.41

84.599

0.393
  pilB Vibrio parahaemolyticus RIMD 2210633

45.897

84.599

0.388
  pilB Haemophilus influenzae 86-028NP

45.478

83.948

0.382
  pilB Haemophilus influenzae Rd KW20

44.703

83.948

0.375
  pilB Acinetobacter baumannii D1279779

43.401

85.466

0.371
  pilF Neisseria gonorrhoeae MS11

44.156

83.514

0.369