Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HUU85_RS15060 Genome accession   NZ_CP058302
Coordinates   3173089..3173529 (-) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain AH65     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3168089..3178529
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUU85_RS15035 (HUU85_15060) coaE 3168579..3169199 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  HUU85_RS15040 (HUU85_15065) - 3169224..3169268 (+) 45 WP_120795372.1 protein YacM -
  HUU85_RS15045 (HUU85_15070) guaC 3169424..3170467 (+) 1044 WP_001217338.1 GMP reductase -
  HUU85_RS15050 (HUU85_15075) hofC 3170502..3171704 (-) 1203 WP_000157266.1 protein transport protein HofC -
  HUU85_RS15055 (HUU85_15080) pilB 3171694..3173079 (-) 1386 WP_126667029.1 type II secretion system protein GspE Machinery gene
  HUU85_RS15060 (HUU85_15085) pilA 3173089..3173529 (-) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  HUU85_RS15065 (HUU85_15090) nadC 3173732..3174625 (-) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HUU85_RS15070 (HUU85_15095) ampD 3174713..3175264 (+) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HUU85_RS15075 (HUU85_15100) ampE 3175261..3176115 (+) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  HUU85_RS15080 (HUU85_15105) aroP 3176158..3177531 (-) 1374 WP_000969915.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=404642 HUU85_RS15060 WP_000360904.1 3173089..3173529(-) (pilA) [Escherichia coli strain AH65]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=404642 HUU85_RS15060 WP_000360904.1 3173089..3173529(-) (pilA) [Escherichia coli strain AH65]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363

Multiple sequence alignment