Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   GK020_RS14145 Genome accession   NZ_CP046392
Coordinates   2910850..2911908 (-) Length   352 a.a.
NCBI ID   WP_045795257.1    Uniprot ID   -
Organism   Acinetobacter indicus strain WMB-7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2905850..2916908
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GK020_RS14120 aroK 2906194..2906736 (-) 543 WP_016658449.1 shikimate kinase AroK -
  GK020_RS14125 pilQ 2906773..2908911 (-) 2139 WP_171501550.1 type IV pilus secretin PilQ Machinery gene
  GK020_RS14130 pilP 2908972..2909499 (-) 528 WP_035363443.1 pilus assembly protein PilP Machinery gene
  GK020_RS14135 pilO 2909499..2910218 (-) 720 WP_045795258.1 type 4a pilus biogenesis protein PilO Machinery gene
  GK020_RS14140 pilN 2910215..2910850 (-) 636 WP_156189589.1 PilN domain-containing protein Machinery gene
  GK020_RS14145 pilM 2910850..2911908 (-) 1059 WP_045795257.1 pilus assembly protein PilM Machinery gene
  GK020_RS14150 ponA 2912079..2914649 (+) 2571 WP_104988081.1 penicillin-binding protein PBP1a -
  GK020_RS14155 - 2914690..2915994 (-) 1305 WP_104988080.1 IS4 family transposase -
  GK020_RS14160 - 2916101..2916904 (-) 804 WP_104988079.1 putative RNA methyltransferase -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 38989.65 Da        Isoelectric Point: 4.5587

>NTDB_id=403818 GK020_RS14145 WP_045795257.1 2910850..2911908(-) (pilM) [Acinetobacter indicus strain WMB-7]
MRRLYRKPSKGLIGVDISSTSVKLLELSVKNDRYWVESYALVPLSEGSMVEKNILNPEAVGDALERAFNLANTPTTAAAL
AIPTSMVISKTIEMDADMTDDEREVQIRMDAEQYIPFPLDEVSLDFEVLPDRLANPNRVNVLLVATRLENVESRCEAIEL
AGLTPKVADVESFAIENAFKVFADTLPMGVNTIGVLDIGHTMTTLSVLKDNKIIYTREQVFGGKQLTQEIQNRYGLSFEE
AGRAKKSRTLPDDYDVEVLEPFLEAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSSKIDIKRIESDASSLMVACGLALRSFD

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=403818 GK020_RS14145 WP_045795257.1 2910850..2911908(-) (pilM) [Acinetobacter indicus strain WMB-7]
GTGCGCAGGTTATATCGTAAACCGAGTAAGGGGTTAATAGGTGTAGATATTAGTTCTACTTCTGTTAAGTTATTGGAGCT
CTCTGTAAAGAATGATCGTTACTGGGTGGAAAGTTACGCCTTGGTACCGCTCTCAGAGGGCAGTATGGTTGAGAAAAATA
TTCTGAATCCTGAAGCTGTAGGGGATGCGCTCGAGCGTGCCTTTAATTTGGCCAATACACCGACAACAGCTGCCGCTTTA
GCGATTCCAACCTCAATGGTAATTAGCAAGACCATTGAGATGGATGCCGATATGACGGATGACGAACGTGAAGTTCAGAT
CCGGATGGATGCAGAGCAATATATTCCTTTCCCATTAGATGAAGTTAGCCTAGACTTTGAGGTACTGCCTGATCGTCTGG
CTAATCCAAACCGGGTCAATGTCTTGCTAGTGGCGACACGACTGGAAAATGTCGAGAGTCGCTGTGAAGCGATTGAACTG
GCCGGTTTAACCCCAAAAGTGGCAGATGTTGAAAGCTTTGCCATTGAAAATGCCTTTAAAGTCTTCGCCGATACCTTGCC
GATGGGGGTAAATACCATTGGTGTGTTAGACATTGGTCATACCATGACCACGCTGTCGGTACTCAAAGACAATAAAATTA
TCTATACCCGCGAGCAGGTCTTTGGCGGCAAGCAGCTGACCCAGGAAATCCAGAACCGTTATGGTCTGTCTTTTGAAGAA
GCCGGCCGCGCCAAGAAAAGCCGTACCTTGCCAGATGATTACGATGTTGAAGTGCTGGAGCCATTCCTTGAAGCGGTGGT
GCAGCAGGCGGCACGTTCGCTGCAGTTCTTCTTCTCCTCATCGCAGTTTAATGAAATTGACCATATTCTGCTGGCCGGCG
GGAATGCCAATATTCCAGGCCTGGCCAAGCTGCTACAGCAAAAACTGGGTTACCGCGTCACCATTGCCAATCCGTTTTTA
CAGATGGGTTTCTCCTCCAAAATTGATATTAAAAGAATTGAAAGCGATGCCTCTTCATTGATGGTGGCATGTGGCTTGGC
ATTGAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

83.807

100

0.838
  comM Acinetobacter nosocomialis M2

83.807

100

0.838
  comM Acinetobacter baylyi ADP1

78.409

100

0.784
  pilM Legionella pneumophila strain ERS1305867

41.62

100

0.423

Multiple sequence alignment