Detailed information NTG0403803

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: GK020_RS08035
• Genome accession: NZ_CP046392
• Coordinates: 1669530..1670723 (-)
• Length: 397 a.a.
• NCBI ID: WP_045794791.1
• Uniprot ID: A0A6C0Y670
• Organism: Acinetobacter indicus strain WMB-7
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1664530..1675723

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GK020_RS08015	-	1666008..1666457 (+)	450	WP_104988575.1	MaoC family dehydratase	-
GK020_RS08020	-	1666561..1667589 (+)	1029	WP_156188986.1	AraC family transcriptional regulator	-
GK020_RS08025	gcvH	1667658..1668032 (+)	375	WP_005178176.1	glycine cleavage system protein GcvH	-
GK020_RS08030	-	1668289..1669470 (+)	1182	WP_156188987.1	MFS transporter	-
GK020_RS08035	pilU	1669530..1670723 (-)	1194	WP_045794791.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
GK020_RS08040	-	1670978..1672033 (-)	1056	WP_156188988.1	3-deoxy-7-phosphoheptulonate synthase	-
GK020_RS08045	-	1672417..1672758 (+)	342	WP_156188989.1	hypothetical protein	-
GK020_RS08050	hppD	1672906..1673964 (+)	1059	WP_156188990.1	4-hydroxyphenylpyruvate dioxygenase	-
GK020_RS08055	-	1674116..1675510 (+)	1395	WP_156188991.1	amino acid permease	-

Sequence

Protein

• Length: 397 a.a.
• Molecular weight: 44672.05 Da
• Isoelectric Point: 6.2356

>NTDB_id=403803 GK020_RS08035 WP_045794791.1 1669530..1670723(-) (pilU) [Acinetobacter indicus strain WMB-7]
MYSAELLEDARKFMFHMLSKVVEFGGSDLFITADFPPSIKHQGMMKALGQQALTAEKTKLFAYSLMNEKQRSEFETDLEC
NFAISVPEVSRFRVNVFQQQLHVGMVIRTISAEIPNFSQLKLPESLKHIIMEKRGLVLVVGGTGSGKSTSLAAMIDHRNE
NSAGHIITVEDPVEYVHKHKKSMITHREVGVDSHSWHNALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
ANNANQTLDRIINFFPEERRNQLLMDLSSNMKAIISQRLVRTEDGKGRRAAVEIMLNTPLISELILKGQFHELKSIMGKS
RELGMQTFDQALFDLYNEGAISYEEAIRNADSMNELRLQIKLKSNRPSEASAAMSFSMLEEPKPEDEDTESAPQQSA

Nucleotide

• Length: 1194 bp

>NTDB_id=403803 GK020_RS08035 WP_045794791.1 1669530..1670723(-) (pilU) [Acinetobacter indicus strain WMB-7]
ATGTACAGTGCAGAATTACTCGAAGATGCGCGTAAATTTATGTTTCATATGCTCAGCAAAGTGGTTGAGTTTGGGGGATC
AGATTTATTTATTACCGCGGATTTTCCACCAAGTATTAAACATCAGGGCATGATGAAAGCGCTGGGACAACAGGCGCTGA
CCGCAGAAAAAACCAAGCTGTTCGCCTACAGCCTCATGAATGAAAAACAGCGCAGTGAATTTGAAACTGATCTGGAATGC
AACTTTGCCATTAGTGTGCCAGAGGTATCGCGTTTTCGGGTCAATGTGTTTCAACAGCAGCTGCATGTCGGGATGGTAAT
TCGAACCATTAGCGCGGAAATTCCGAACTTTAGCCAGCTGAAACTGCCGGAATCGCTGAAGCATATTATTATGGAAAAGC
GCGGTCTGGTGCTGGTGGTGGGGGGAACCGGTTCAGGTAAATCGACCTCTTTGGCGGCGATGATTGATCATCGTAATGAA
AATTCAGCCGGGCATATTATTACCGTGGAAGACCCGGTGGAATATGTGCACAAGCATAAGAAGTCGATGATTACCCATCG
TGAAGTCGGGGTCGACAGCCATTCCTGGCACAATGCGCTGAAAAATACCTTACGTCAGGCACCGGATGTGATTTTAATTG
GCGAGATCCGCGACACCGAAACCATGGAACATGCGATTGCCTTTGCTGAAACCGGGCATTTATGTTTGGGCACCTTGCAT
GCCAATAACGCCAACCAGACGCTGGACCGGATTATCAACTTTTTCCCGGAAGAGCGCCGTAACCAGTTGCTGATGGATCT
GTCGTCCAATATGAAAGCCATTATTTCCCAGCGTCTGGTGCGTACCGAAGATGGCAAAGGCCGCCGTGCGGCAGTCGAGA
TTATGCTGAATACGCCGCTGATTTCTGAACTGATTTTAAAAGGCCAGTTCCATGAGTTAAAGTCGATTATGGGTAAATCG
CGTGAACTGGGTATGCAGACGTTTGATCAGGCCTTATTTGATCTGTATAACGAAGGTGCAATTTCCTATGAAGAAGCAAT
TCGTAATGCGGACTCGATGAATGAATTACGCTTGCAGATCAAGCTGAAAAGTAACCGGCCAAGCGAAGCTTCGGCAGCTA
TGAGTTTTAGCATGCTGGAAGAGCCAAAACCGGAAGATGAAGATACGGAATCTGCTCCTCAACAGAGTGCTTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (117-282)

3D structure

• AlphaFold DB: A0A6C0Y670

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	57.568	93.199	0.537
pilU	Acinetobacter baylyi ADP1	54.42	91.184	0.496
pilU	Vibrio cholerae strain A1552	52.663	85.139	0.448
pilT	Acinetobacter baumannii strain A118	43.939	83.123	0.365
pilT	Acinetobacter nosocomialis M2	43.939	83.123	0.365
pilT	Acinetobacter baumannii D1279779	43.939	83.123	0.365
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.814	84.131	0.36