Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   GKO36_RS18620 Genome accession   NZ_CP046145
Coordinates   3731505..3732890 (-) Length   461 a.a.
NCBI ID   WP_014419012.1    Uniprot ID   I2CAS2
Organism   Bacillus velezensis strain PEBA20     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3726505..3737890
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GKO36_RS18585 (GKO36_18460) flgL 3726680..3727597 (-) 918 WP_014419007.1 flagellar hook-associated protein FlgL -
  GKO36_RS18590 (GKO36_18465) flgK 3727609..3729126 (-) 1518 WP_014419008.1 flagellar hook-associated protein FlgK -
  GKO36_RS18595 (GKO36_18470) - 3729142..3729624 (-) 483 WP_003151394.1 flagellar protein FlgN -
  GKO36_RS18600 (GKO36_18475) flgM 3729639..3729905 (-) 267 WP_003151393.1 flagellar biosynthesis anti-sigma factor FlgM -
  GKO36_RS18605 (GKO36_18480) - 3729975..3730394 (-) 420 WP_014419009.1 TIGR03826 family flagellar region protein -
  GKO36_RS18610 (GKO36_18485) comFC 3730468..3731157 (-) 690 WP_197535984.1 ComF family protein Machinery gene
  GKO36_RS18615 (GKO36_18490) - 3731163..3731447 (-) 285 WP_014419011.1 competence protein ComFB -
  GKO36_RS18620 (GKO36_18495) comFA 3731505..3732890 (-) 1386 WP_014419012.1 DEAD/DEAH box helicase Machinery gene
  GKO36_RS18625 (GKO36_18500) - 3732998..3733846 (-) 849 WP_003151387.1 DegV family protein -
  GKO36_RS18630 (GKO36_18505) degU 3733944..3734633 (-) 690 WP_003219701.1 two-component system response regulator DegU Regulator
  GKO36_RS18635 (GKO36_18510) degS 3734710..3735873 (-) 1164 WP_007407484.1 two-component sensor histidine kinase DegS Regulator
  GKO36_RS18640 (GKO36_18515) - 3736096..3736743 (+) 648 WP_007614107.1 YigZ family protein -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52599.04 Da        Isoelectric Point: 10.2804

>NTDB_id=401657 GKO36_RS18620 WP_014419012.1 3731505..3732890(-) (comFA) [Bacillus velezensis strain PEBA20]
MSEGASEFSEVRDFFYGRHLLRSEIPFSDQRIKRLTEKEYITAEPSIIRRKNRYVCQRCGQSDQASFAAFWAPSAKRQIT
YCRACVMMGRADELTSLYSWNQAPENSWEPVKLSWEGTLTDGQKRAAAALTDAIKERQELLVWAVCGSGKTEMLFPGIEF
ALNHGLRVCVATPRTDVVLELLPRLNKAFEKVEVSALYGGSEDKGRLTPLMISTAHQLMRYRDIFDVMIIDEVDAFPFSA
DETLRFAVDKARKKNSALVYVTATPSDTLKKKAEAGLLKSVRIPARYHRKPLPEPRFLWCGNWKKKLQKGKLPRSVTDWV
RQKLQLQLPVFLFVPSVHVLKKTTDYFRKLNIRAEGVHAEDTFRKDKVKRFRDGRLDLLVTTTILERGVTVPKVQTCVLG
AEAPIFTESALVQIAGRTGRHYKHFSGDVVMFHFGITNGMKKAKKHIEHMNKLAQKSKLLD

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=401657 GKO36_RS18620 WP_014419012.1 3731505..3732890(-) (comFA) [Bacillus velezensis strain PEBA20]
ATGTCCGAAGGTGCTTCAGAATTTTCAGAGGTGCGGGATTTCTTTTACGGCCGGCATTTATTAAGATCTGAGATACCGTT
TTCAGACCAGCGCATCAAGCGTTTAACAGAAAAAGAATATATAACGGCCGAGCCGTCAATCATCCGCCGGAAAAACCGTT
ACGTCTGCCAACGATGCGGTCAGTCTGATCAAGCCAGCTTTGCGGCATTTTGGGCGCCCTCTGCCAAAAGGCAGATTACC
TATTGCCGCGCCTGTGTCATGATGGGAAGGGCTGATGAACTGACATCATTATATTCTTGGAATCAAGCCCCTGAAAACAG
CTGGGAGCCTGTAAAGCTTTCTTGGGAAGGAACACTGACAGACGGACAGAAGCGGGCGGCCGCCGCGCTTACAGATGCGA
TAAAAGAGAGACAAGAGCTGTTAGTATGGGCGGTTTGCGGATCGGGGAAAACAGAGATGCTGTTTCCGGGTATCGAGTTT
GCGCTGAATCACGGTTTGCGCGTTTGTGTCGCAACTCCGCGTACAGATGTCGTACTTGAACTGCTGCCGAGACTGAATAA
AGCATTTGAGAAAGTCGAAGTTTCTGCTTTGTACGGCGGCAGCGAGGACAAAGGCCGCTTGACTCCGCTGATGATTTCAA
CCGCTCACCAGCTGATGCGCTACCGAGACATTTTTGATGTCATGATAATAGATGAAGTAGATGCGTTTCCTTTTTCAGCC
GATGAGACATTAAGATTCGCTGTTGATAAAGCGAGGAAGAAAAACAGCGCCCTTGTTTATGTAACGGCGACTCCTTCAGA
CACATTAAAAAAGAAGGCTGAAGCCGGTTTGCTGAAAAGCGTCCGCATACCGGCCAGATATCATCGGAAACCCCTCCCGG
AGCCGCGTTTTTTGTGGTGCGGCAATTGGAAGAAGAAGCTTCAGAAAGGAAAACTGCCGCGCTCTGTAACGGATTGGGTC
CGCCAAAAACTTCAATTACAGCTGCCGGTTTTCTTATTCGTCCCGTCTGTACACGTATTGAAAAAGACAACGGATTATTT
TCGGAAATTAAACATACGGGCAGAAGGCGTACATGCGGAAGACACATTCAGAAAGGATAAAGTGAAACGATTCAGAGACG
GCCGGCTTGACCTGCTGGTAACGACTACGATTTTAGAAAGAGGGGTTACCGTGCCCAAAGTTCAGACATGTGTCCTCGGC
GCAGAAGCACCTATCTTTACTGAGAGCGCGCTCGTACAGATCGCGGGCAGAACGGGAAGGCATTATAAACATTTCAGCGG
TGATGTCGTCATGTTTCATTTCGGAATTACAAACGGAATGAAAAAAGCAAAAAAACATATAGAACATATGAACAAATTGG
CACAAAAAAGTAAATTGTTGGACTAG

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(118-266)

Helicase conserved C-terminal domain (Helicase_C)

(330-421)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I2CAS2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

62.391

99.783

0.623

Multiple sequence alignment