Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   GKC25_RS05430 Genome accession   NZ_CP046128
Coordinates   1125029..1126855 (+) Length   608 a.a.
NCBI ID   WP_302621039.1    Uniprot ID   -
Organism   Bacillus pumilus strain V17C11     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 1120029..1131855
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GKC25_RS05410 (GKC25_05390) - 1120293..1120958 (-) 666 WP_034663251.1 TerC family protein -
  GKC25_RS05415 (GKC25_05395) mecA 1121221..1121886 (+) 666 WP_012009573.1 adaptor protein MecA Regulator
  GKC25_RS05420 (GKC25_05400) cls 1121972..1123492 (+) 1521 WP_034663247.1 cardiolipin synthase -
  GKC25_RS05425 (GKC25_05405) - 1123654..1124814 (+) 1161 WP_034663244.1 competence protein CoiA family protein -
  GKC25_RS05430 (GKC25_05410) pepF 1125029..1126855 (+) 1827 WP_302621039.1 oligoendopeptidase F Regulator
  GKC25_RS05435 - 1126959..1127132 (-) 174 WP_034663238.1 hypothetical protein -
  GKC25_RS05440 (GKC25_05415) - 1127429..1128340 (-) 912 WP_034663234.1 ClpXP adapter SpxH family protein -
  GKC25_RS05445 (GKC25_05420) - 1128337..1128735 (-) 399 WP_003211417.1 thiol management oxidoreductase -
  GKC25_RS05450 (GKC25_05425) - 1129012..1129779 (-) 768 WP_034663232.1 lytic transglycosylase domain-containing protein -
  GKC25_RS05455 (GKC25_05430) - 1129801..1130379 (-) 579 WP_034663230.1 CYTH domain-containing protein -
  GKC25_RS05460 (GKC25_05435) - 1130616..1130981 (+) 366 WP_034663228.1 hypothetical protein -
  GKC25_RS05465 (GKC25_05440) - 1131015..1131644 (+) 630 WP_034663227.1 GTP pyrophosphokinase family protein -

Sequence

Protein


Download         Length: 608 a.a.        Molecular weight: 70146.81 Da        Isoelectric Point: 5.3592

>NTDB_id=401372 GKC25_RS05430 WP_302621039.1 1125029..1126855(+) (pepF) [Bacillus pumilus strain V17C11]
MTSQSKNNHLPDRSEVKEENKWRLEDIFESVDAWNKEFEAVKKEIPKLAQFKGKLAHSADVLYEALTFQDQLSEKLGKLY
TYAHMKYDEDTTNSSFQALNDKASNLFTQLSSTSAYIVPEILSIQEDKLQQFILEKEELKLYSHALEEINKERPHILSEE
QEALLAEASEPLSTSSTTFSMFNNADISFPSVKDENGEEKKITHGNFITFLNSDDREVRKNAFKAVYKTYDQYKNTLAST
LSGSIKKDNFYAKVRNYNSAREAALSRNSIPEEVYDNLIDTVHQYLPLLHRYIELRKKVLKLDEVHNYDLYTPLVKDAGM
KLTYDEAKDYMLKGLAPLGEEYVSVLKEGLSNRWVDVYENKGKRSGAYSSGSYGTNPYILMNWQNNIDNLFTLAHEFGHS
VHSYYTRKHQPYPYGNYSIFVAEVASTTNEALLGEYLLNHLDDKKQRLYVLNHLLEGFRGTVFRQTMFAEFEHLIHVKAQ
EGEALTPEFMTNLYYDLNKKYFGDGMVVDKEIGLEWTRIPHFYYNYYVYQYATGYSAAQALSKQILEEGNPAVERYTDFL
KAGSSDYPINVLKKAGVDMASKEPIKAACQLFEEKLKEMEELISKTGE

Nucleotide


Download         Length: 1827 bp        

>NTDB_id=401372 GKC25_RS05430 WP_302621039.1 1125029..1126855(+) (pepF) [Bacillus pumilus strain V17C11]
ATGACTTCACAAAGCAAAAATAACCATTTACCAGACAGAAGCGAAGTAAAAGAAGAAAACAAATGGCGTCTTGAAGATAT
TTTCGAAAGCGTTGATGCATGGAACAAGGAATTCGAAGCTGTCAAAAAAGAAATTCCTAAGCTAGCCCAATTTAAAGGCA
AGCTGGCCCATTCTGCTGATGTGCTGTATGAAGCCCTAACCTTTCAGGATCAGCTTTCTGAAAAGCTTGGTAAGCTTTAT
ACATATGCGCACATGAAATACGATGAGGATACAACTAACTCATCATTTCAAGCGCTGAATGATAAAGCATCGAATTTATT
TACACAGCTCTCTAGTACTTCTGCTTATATTGTTCCAGAGATTTTATCGATTCAAGAAGATAAGTTGCAGCAGTTTATTC
TGGAAAAAGAAGAATTAAAACTGTATTCCCATGCTCTCGAAGAAATTAATAAAGAGCGTCCACATATTTTAAGTGAGGAG
CAAGAAGCTTTATTAGCTGAGGCCTCTGAACCACTCTCAACTTCATCTACTACTTTTAGTATGTTTAACAATGCTGATAT
TTCGTTCCCTTCTGTAAAGGATGAAAATGGGGAAGAAAAGAAGATTACCCACGGCAACTTTATCACATTTTTAAACAGTG
ATGACCGTGAAGTGAGAAAGAATGCCTTTAAAGCTGTGTATAAAACGTATGATCAGTATAAAAACACGCTTGCTTCGACA
CTGAGCGGTTCGATTAAAAAGGATAATTTTTATGCAAAAGTACGAAATTATAACTCAGCAAGAGAAGCGGCACTATCTCG
GAACAGTATTCCAGAAGAAGTATATGACAATCTGATTGATACAGTTCATCAATATTTACCGTTATTACACCGTTATATTG
AATTGCGTAAAAAGGTATTAAAGCTTGATGAAGTGCACAATTATGACTTATATACACCACTTGTAAAAGATGCTGGTATG
AAGCTAACCTACGATGAAGCAAAGGATTATATGCTAAAAGGGTTAGCGCCATTAGGAGAAGAATATGTGTCCGTGTTAAA
AGAGGGACTGAGCAATCGCTGGGTAGACGTTTATGAGAATAAAGGGAAACGCAGCGGTGCGTATTCATCTGGAAGCTATG
GCACAAATCCATATATCCTCATGAACTGGCAAAATAATATTGATAATTTATTTACTCTGGCTCATGAATTTGGGCACTCT
GTCCACAGCTACTATACAAGAAAGCACCAGCCATATCCGTATGGAAACTATAGCATCTTTGTAGCTGAAGTGGCTTCTAC
GACAAATGAAGCGTTACTAGGCGAATACCTGCTGAATCACCTAGATGATAAAAAGCAGCGTTTGTATGTGTTAAATCACT
TGTTAGAAGGATTTAGAGGAACGGTATTCAGACAGACGATGTTTGCTGAGTTTGAGCATCTTATTCATGTGAAAGCGCAA
GAAGGAGAGGCTTTAACGCCGGAATTTATGACGAACCTTTATTACGACCTGAATAAGAAGTATTTTGGAGACGGCATGGT
TGTGGATAAAGAAATCGGATTAGAATGGACGAGAATTCCTCACTTCTATTACAACTATTATGTCTATCAATATGCAACAG
GCTATAGTGCTGCTCAGGCATTAAGCAAGCAAATATTAGAAGAAGGAAATCCTGCGGTGGAGCGCTACACAGACTTCCTT
AAAGCAGGAAGCTCTGATTATCCAATCAATGTGTTGAAAAAAGCAGGCGTTGATATGGCTTCCAAAGAACCAATCAAAGC
AGCATGTCAATTGTTCGAAGAAAAGCTGAAAGAAATGGAAGAGCTCATCTCTAAAACGGGTGAGTGA

Domains

Predicted by InterproScan.

Peptidase family M3 (Peptidase_M3)

(211-590)

Oligopeptidase F (Peptidase_M3_N)

(120-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

49.158

97.697

0.48

Multiple sequence alignment