Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   POR16_RS20200 Genome accession   NZ_CP046035
Coordinates   4500387..4502081 (+) Length   564 a.a.
NCBI ID   WP_005895655.1    Uniprot ID   A0AAE6QIZ1
Organism   Pseudomonas coronafaciens pv. oryzae str. 1_6     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4495387..4507081
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  POR16_RS20175 (POR16_20175) - 4496514..4497512 (-) 999 WP_005895665.1 ATP-grasp domain-containing protein -
  POR16_RS20180 (POR16_20180) - 4497632..4498177 (+) 546 WP_005895663.1 DUF1523 family protein -
  POR16_RS20185 (POR16_20185) - 4498221..4498628 (-) 408 WP_005895661.1 hypothetical protein -
  POR16_RS20190 (POR16_20190) - 4498625..4499299 (-) 675 WP_122358061.1 MerR family transcriptional regulator -
  POR16_RS20195 (POR16_20195) - 4499756..4500160 (-) 405 WP_005895657.1 pilin -
  POR16_RS20200 (POR16_20200) pilB 4500387..4502081 (+) 1695 WP_005895655.1 type IV-A pilus assembly ATPase PilB Machinery gene
  POR16_RS20205 (POR16_20205) pilC 4502084..4503301 (+) 1218 WP_005895653.1 type II secretion system F family protein Machinery gene
  POR16_RS20210 (POR16_20210) pilD 4503303..4504175 (+) 873 WP_005895651.1 prepilin peptidase Machinery gene
  POR16_RS20215 (POR16_20215) coaE 4504172..4504795 (+) 624 WP_005895649.1 dephospho-CoA kinase -
  POR16_RS20220 (POR16_20220) yacG 4504792..4505001 (+) 210 WP_005895648.1 DNA gyrase inhibitor YacG -
  POR16_RS20225 (POR16_20225) - 4505145..4505774 (+) 630 WP_005895647.1 DUF1780 domain-containing protein -
  POR16_RS20230 (POR16_20230) - 4505771..4506304 (+) 534 WP_005895644.1 MOSC domain-containing protein -

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 62448.69 Da        Isoelectric Point: 6.2549

>NTDB_id=400391 POR16_RS20200 WP_005895655.1 4500387..4502081(+) (pilB) [Pseudomonas coronafaciens pv. oryzae str. 1_6]
MTDAVLTGLAKQLVLAELLTETVAQQAYQQARRDKISLVSYLVQNKLVKSLTLAEMASDQFGVPFMDLASLDKESQPKGL
VSEKLIRQHHALPLWRRGNKLFIGISDPTNHQAVTDIQFSTGLNTEAILVEDDKLTTAIDRFFDNDSGLGNLEDVDLGLD
IEPADGKETSLAAQNDADDAPVVRFVNKMLLDAIRLGSSDLHFEPYEKIFRVRLRTDGILHEVARPPIHLANRIAARLKV
MASLDISERRKPQDGRVKLRVSKTKAIDFRMNTLPTLWGEKIVMRILDPTSAQMGIDALGYEPEQKALYLEALKQPQGMI
LVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPRQGLDFSQALRAFLRQDPDVIMVGEIRDLET
AEIAIKASQTGHMVLSTLHTNSAAETLTRLHHMGVAAFNIATAINLIIAQRLARKLCSHCKKEIDIPRETLIQEGFPESK
IGTFKIFGPVGCEHCNGGYRGRVGIYEVVKKTAELERIIMEEGNSLEISRQMRKDGFNDLRTSGLSKAMQGITSLEEVNR
VTKD

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=400391 POR16_RS20200 WP_005895655.1 4500387..4502081(+) (pilB) [Pseudomonas coronafaciens pv. oryzae str. 1_6]
ATGACTGATGCTGTACTCACTGGCTTGGCCAAACAATTGGTCCTTGCCGAGCTGCTTACCGAAACCGTTGCGCAGCAAGC
GTATCAACAAGCGCGCCGCGACAAGATTTCGTTGGTGAGCTATCTGGTTCAGAACAAGCTGGTCAAAAGCCTGACGCTCG
CAGAGATGGCTTCGGATCAGTTTGGTGTCCCTTTTATGGATCTGGCCAGCCTGGACAAGGAAAGCCAGCCTAAGGGGCTG
GTGAGTGAAAAGCTTATTCGACAGCATCATGCCCTTCCACTATGGCGAAGAGGTAACAAGCTGTTTATCGGTATTTCGGA
CCCGACTAACCATCAGGCGGTGACCGATATTCAGTTCAGCACCGGCCTGAACACTGAAGCGATTCTGGTCGAAGACGACA
AGCTGACCACTGCGATAGACCGTTTTTTTGACAATGACAGCGGGCTGGGCAACCTGGAAGATGTCGATCTGGGTCTGGAT
ATCGAACCCGCTGATGGCAAGGAAACCTCCCTTGCTGCACAGAATGATGCTGACGACGCCCCGGTGGTGCGCTTCGTCAA
CAAAATGCTGTTGGATGCTATCAGGCTAGGATCATCTGACCTGCACTTCGAGCCCTATGAAAAGATCTTTCGTGTGCGCT
TGCGTACCGACGGCATTCTGCACGAAGTCGCCAGGCCGCCGATTCATCTGGCAAACCGTATTGCTGCCCGCTTGAAAGTC
ATGGCCAGCCTCGATATTTCCGAGCGACGTAAACCGCAGGATGGCCGGGTCAAGTTAAGGGTGTCCAAAACAAAGGCCAT
CGATTTCCGTATGAACACCTTGCCCACGCTATGGGGCGAGAAGATCGTGATGCGTATTCTTGATCCGACCAGTGCGCAGA
TGGGCATTGACGCTCTGGGCTATGAACCAGAGCAAAAGGCACTGTATCTGGAGGCGCTAAAGCAGCCACAAGGCATGATT
CTTGTGACCGGCCCTACGGGTTCTGGCAAGACTGTTTCCCTGTATACCGGGCTGAATATCCTCAATACTGTGGACATCAA
CATTTCCACCGCTGAAGACCCGGTAGAGATCAACCTTGAAGGCATCAATCAGGTCAACGTCAACCCACGCCAAGGACTCG
ACTTTTCTCAGGCATTGCGCGCCTTCTTGCGTCAAGATCCCGACGTGATCATGGTCGGCGAGATACGTGATTTGGAAACT
GCCGAGATCGCCATCAAGGCATCACAGACAGGCCATATGGTGCTTTCAACGCTGCACACCAACAGCGCAGCAGAAACCCT
GACGCGTTTACATCACATGGGCGTCGCTGCATTCAACATTGCCACAGCGATCAACCTGATTATCGCGCAACGACTTGCAC
GCAAACTTTGCAGCCACTGCAAGAAAGAAATCGATATTCCGCGGGAGACGTTGATTCAGGAAGGCTTCCCGGAATCAAAA
ATCGGAACGTTCAAAATCTTTGGCCCCGTGGGCTGCGAACATTGCAACGGCGGCTATAGAGGCCGGGTAGGTATTTACGA
AGTGGTCAAAAAAACAGCAGAGCTGGAACGCATTATCATGGAAGAAGGGAACTCGCTTGAGATATCCAGACAGATGCGCA
AAGACGGTTTCAATGATCTGCGCACTTCGGGCTTGTCAAAAGCGATGCAAGGCATCACCAGCCTTGAAGAAGTCAACCGC
GTAACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.951

100

0.582
  pilB Acinetobacter baylyi ADP1

57.067

100

0.573
  pilB Legionella pneumophila strain ERS1305867

55.929

100

0.56
  pilB Vibrio cholerae strain A1552

51.423

99.645

0.512
  pilB Vibrio parahaemolyticus RIMD 2210633

51.071

99.291

0.507
  pilB Vibrio campbellii strain DS40M4

49.647

100

0.498
  pilF Neisseria gonorrhoeae MS11

47.865

99.645

0.477
  pilF Thermus thermophilus HB27

38.516

100

0.387
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.783

93.262

0.362

Multiple sequence alignment