Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   GJE16_RS09675 Genome accession   NZ_CP045931
Coordinates   1886116..1887132 (-) Length   338 a.a.
NCBI ID   WP_075270915.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain AUSMDU00010538     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1881116..1892132
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GJE16_RS11270 - 1881194..1881325 (-) 132 WP_000768904.1 hypothetical protein -
  GJE16_RS09630 (GJE16_09630) - 1881326..1882516 (-) 1191 WP_000167766.1 acetate kinase -
  GJE16_RS09635 (GJE16_09635) comYH 1882567..1883520 (-) 954 WP_000345117.1 class I SAM-dependent methyltransferase Machinery gene
  GJE16_RS09645 (GJE16_09645) - 1883581..1884174 (-) 594 Protein_1867 class I SAM-dependent methyltransferase -
  GJE16_RS09650 (GJE16_09650) comGG/cglG 1884311..1884724 (-) 414 WP_000265630.1 competence type IV pilus minor pilin ComGG Machinery gene
  GJE16_RS09655 (GJE16_09655) comGF/cglF 1884702..1885163 (-) 462 WP_000250530.1 competence type IV pilus minor pilin ComGF Machinery gene
  GJE16_RS09660 (GJE16_09660) comGE/cglE 1885126..1885428 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  GJE16_RS09665 (GJE16_09665) comGD/cglD 1885391..1885795 (-) 405 WP_000588006.1 competence type IV pilus minor pilin ComGD Machinery gene
  GJE16_RS09670 (GJE16_09670) comGC/cglC 1885788..1886114 (-) 327 WP_000738638.1 comG operon protein ComGC Machinery gene
  GJE16_RS09675 (GJE16_09675) comGB/cglB 1886116..1887132 (-) 1017 WP_075270915.1 competence type IV pilus assembly protein ComGB Machinery gene
  GJE16_RS09680 (GJE16_09680) comGA/cglA/cilD 1887080..1888021 (-) 942 WP_000249556.1 competence type IV pilus ATPase ComGA Machinery gene
  GJE16_RS09685 (GJE16_09685) - 1888096..1888461 (-) 366 WP_000286415.1 DUF1033 family protein -
  GJE16_RS09690 (GJE16_09690) - 1888612..1889670 (-) 1059 WP_000649471.1 zinc-dependent alcohol dehydrogenase family protein -
  GJE16_RS09695 (GJE16_09695) nagA 1889833..1890984 (-) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38496.55 Da        Isoelectric Point: 9.6162

>NTDB_id=399443 GJE16_RS09675 WP_075270915.1 1886116..1887132(-) (comGB/cglB) [Streptococcus pneumoniae strain AUSMDU00010538]
MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSALLDKQCVTQMRAGLSQGKSFSEMMESLGCSSAI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLFFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEI
GQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=399443 GJE16_RS09675 WP_075270915.1 1886116..1887132(-) (comGB/cglB) [Streptococcus pneumoniae strain AUSMDU00010538]
ATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTATTTAA
CAATCTCTTTTCTAGCGGTTTTCATCTGGTGGAGACCATCTCCTTTTTAGATAGGAGTGCTTTGTTGGACAAGCAGTGTG
TGACCCAGATGCGTGCGGGCTTGTCTCAGGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGATGTTCAAGTGCTATT
GTCACTCAGTTATCCCTAGCTGAAGTCCATGGCAATCTCCACCTGAGTTTGGGAAAGATAGAAGAGTATCTAGACAATCT
GGCCAAGGTCAAGAAAAAATTAATTGAAGTAGCGACCTATCCTTTGATTTTGCTGTTTTTTCTTCTCTTAATTATGCTGG
GGCTACGGAATTACCTGCTCCCACAACTGGATAGTAGCAATATTGCCACCCGAATTATCGGTAATCTGCCACAAATTTTT
CTAGGCATGGTAGGGCTTGTTTCCGTGCTTGCCCTTTTAGCACTAACTTTTTATAAAAGAAGTTCTAAAATGAGTGTCTT
TTCTATCTTAGCACGCCTTCCCTTTATTGGAATCTTTGTGCAGACCTACTTGACAGCCTATTATGCACGTGAATGGGGGA
ATATGATTTCACAGGGAATGGAGCTGACGCAGATTTTTCAAATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAATC
GGTCAAGATCTGGCTCAAACCCTGAAAAATGGCCGTGAATTTTCTCAGACGATAGGAACCTATCCTTTCTTTAGGAAGGA
ATTGAGTCTCATCATAGAGTATGGGGAAGTTAAGTCCAAGCTGGGTAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATCTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus pneumoniae Rx1

98.817

100

0.988
  comGB/cglB Streptococcus pneumoniae D39

98.817

100

0.988
  comGB/cglB Streptococcus pneumoniae R6

98.817

100

0.988
  comGB/cglB Streptococcus pneumoniae TIGR4

98.817

100

0.988
  comGB/cglB Streptococcus mitis SK321

94.97

100

0.95
  comGB/cglB Streptococcus mitis NCTC 12261

94.675

100

0.947
  comYB Streptococcus gordonii str. Challis substr. CH1

70.833

99.408

0.704
  comYB Streptococcus mutans UA140

58.176

94.083

0.547
  comYB Streptococcus mutans UA159

58.176

94.083

0.547
  comGB Lactococcus lactis subsp. cremoris KW2

50.898

98.817

0.503

Multiple sequence alignment