Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HPE49_RS18770 Genome accession   NZ_CP054363
Coordinates   3898378..3899763 (-) Length   461 a.a.
NCBI ID   WP_174144518.1    Uniprot ID   -
Organism   Escherichia coli strain SCU-171     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3893378..3904763
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPE49_RS18740 (HPE49_18735) mutT 3893713..3894111 (+) 399 WP_000736051.1 8-oxo-dGTP diphosphatase MutT -
  HPE49_RS18745 (HPE49_18740) yacG 3894313..3894510 (-) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  HPE49_RS18750 (HPE49_18745) zapD 3894520..3895263 (-) 744 WP_042106188.1 cell division protein ZapD -
  HPE49_RS18755 (HPE49_18750) coaE 3895263..3895883 (-) 621 WP_001269512.1 dephospho-CoA kinase -
  HPE49_RS26590 - 3895908..3895952 (+) 45 WP_120795372.1 protein YacM -
  HPE49_RS18760 (HPE49_18755) guaC 3896108..3897151 (+) 1044 WP_001217338.1 GMP reductase -
  HPE49_RS18765 (HPE49_18760) hofC 3897186..3898388 (-) 1203 WP_042106187.1 protein transport protein HofC -
  HPE49_RS18770 (HPE49_18765) pilB 3898378..3899763 (-) 1386 WP_174144518.1 type II secretion system protein GspE Machinery gene
  HPE49_RS18775 (HPE49_18770) pilA 3899773..3900213 (-) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  HPE49_RS18780 (HPE49_18775) nadC 3900416..3901309 (-) 894 WP_001135153.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HPE49_RS18785 (HPE49_18780) ampD 3901397..3901948 (+) 552 WP_000923730.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HPE49_RS18790 (HPE49_18785) ampE 3901945..3902799 (+) 855 WP_000171975.1 beta-lactamase regulator AmpE -
  HPE49_RS18795 (HPE49_18790) aroP 3902842..3904212 (-) 1371 WP_000399017.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50619.36 Da        Isoelectric Point: 6.4971

>NTDB_id=399075 HPE49_RS18770 WP_174144518.1 3898378..3899763(-) (pilB) [Escherichia coli strain SCU-171]
MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTHQQMEGHASLTQQTLPVAIHEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVLPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAISFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLVLVTGPTGSGKTVTLYSALQTLN
TADINICSVEDPVEIPIAGLNQTQIHSRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TCETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPRWQAPGCVHCYHGFYGRTALFE
VLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=399075 HPE49_RS18770 WP_174144518.1 3898378..3899763(-) (pilB) [Escherichia coli strain SCU-171]
ATGAATATTCCACAGCTCACCGCCCTGTGTCTGCGTTATCAGGGAGTCTTGCTGGATGCCAGCGAAGAGGTGGTTCATGT
TGCGGTAGTCGATGCACCTTCGCATGAGCTACTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCACCAACAAATGGAAGGTCACGCCAGTCTCACCCAACAGACATTGCCCGTAGCTATTCATGAGAAGCATCAGCCC
AAAGCAGAGTTGCTGACTCGAACGTTACAATCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGTGTATTGCATCCTTTACCGGATGTTTTACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGCAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCATCTCATTTCGTATTGCGACCTTACCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGAGTCAGGCACTGGATGTCAACACGCTTGGAATGCAGCCGTTACAACTGGCGGACTTTGCTCATGCCTTGCAACAAC
CACAGGGACTGGTGCTGGTAACTGGCCCTACAGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAACGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCGGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATTC
GCGTGCCGGACTCACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGATCCTGACGTCATCATGATCGGAGAGATCC
GCGATGGCGAAACGGCAGAAATTGCCATTAAAGCCGCGCAAACCGGTCACCTGGTGTTGTCTACCCTACACACTAATTCC
ACCTGCGAAACGCTGGTACGTTTACAGCAAATGGGAGTCGCCCGCTGGATGCTCTCATCGGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGCAAACTTTGCCCACATTGTCGCCGACAGCAAGGGGAGCCAATCCATATTCCAGACAATGTATGGC
CGTCGCCGCTGCCCCGCTGGCAAGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGCACGGCCTTATTTGAA
GTTCTGCCCATAACACCGGTCATACGTCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACGCACGCCCGACA
GGCGGGTATGCGAACGCTTTTTGAAAACGGCTGTCTGGCCGTGGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TATTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(88-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

42.65

100

0.447
  pilB Vibrio parahaemolyticus RIMD 2210633

40.452

100

0.427
  pilB Legionella pneumophila strain ERS1305867

49.738

82.863

0.412
  pilB Acinetobacter baylyi ADP1

40.385

100

0.41
  pilB Glaesserella parasuis strain SC1401

40.821

100

0.41
  pilB Acinetobacter baumannii D1279779

38.413

100

0.399
  pilB Vibrio cholerae strain A1552

46.667

84.599

0.395
  pilB Haemophilus influenzae 86-028NP

44.444

83.948

0.373
  pilF Neisseria gonorrhoeae MS11

44.156

83.514

0.369
  pilB Haemophilus influenzae Rd KW20

43.669

83.948

0.367