Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HPE49_RS18775 Genome accession   NZ_CP054363
Coordinates   3899773..3900213 (-) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain SCU-171     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3894773..3905213
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPE49_RS18755 (HPE49_18750) coaE 3895263..3895883 (-) 621 WP_001269512.1 dephospho-CoA kinase -
  HPE49_RS26590 - 3895908..3895952 (+) 45 WP_120795372.1 protein YacM -
  HPE49_RS18760 (HPE49_18755) guaC 3896108..3897151 (+) 1044 WP_001217338.1 GMP reductase -
  HPE49_RS18765 (HPE49_18760) hofC 3897186..3898388 (-) 1203 WP_042106187.1 protein transport protein HofC -
  HPE49_RS18770 (HPE49_18765) pilB 3898378..3899763 (-) 1386 WP_174144518.1 type II secretion system protein GspE Machinery gene
  HPE49_RS18775 (HPE49_18770) pilA 3899773..3900213 (-) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  HPE49_RS18780 (HPE49_18775) nadC 3900416..3901309 (-) 894 WP_001135153.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HPE49_RS18785 (HPE49_18780) ampD 3901397..3901948 (+) 552 WP_000923730.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HPE49_RS18790 (HPE49_18785) ampE 3901945..3902799 (+) 855 WP_000171975.1 beta-lactamase regulator AmpE -
  HPE49_RS18795 (HPE49_18790) aroP 3902842..3904212 (-) 1371 WP_000399017.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=399076 HPE49_RS18775 WP_000360904.1 3899773..3900213(-) (pilA) [Escherichia coli strain SCU-171]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=399076 HPE49_RS18775 WP_000360904.1 3899773..3900213(-) (pilA) [Escherichia coli strain SCU-171]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTTCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363

Multiple sequence alignment