Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HR073_RS03180 Genome accession   NZ_CP054227
Coordinates   690395..691780 (-) Length   461 a.a.
NCBI ID   WP_001025168.1    Uniprot ID   -
Organism   Escherichia coli strain EcPF15     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 685395..696780
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HR073_RS03145 (HR073_03145) mutT 685844..686242 (+) 399 WP_000736038.1 8-oxo-dGTP diphosphatase MutT -
  HR073_RS03150 (HR073_03150) yacG 686330..686527 (-) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  HR073_RS03155 (HR073_03155) zapD 686537..687280 (-) 744 WP_001194720.1 cell division protein ZapD -
  HR073_RS03160 (HR073_03160) coaE 687280..687900 (-) 621 WP_001269513.1 dephospho-CoA kinase -
  HR073_RS03165 - 687925..687969 (+) 45 WP_120795372.1 protein YacM -
  HR073_RS03170 (HR073_03165) guaC 688125..689168 (+) 1044 WP_001217337.1 GMP reductase -
  HR073_RS03175 (HR073_03170) hofC 689203..690405 (-) 1203 WP_000157287.1 protein transport protein HofC -
  HR073_RS03180 (HR073_03175) pilB 690395..691780 (-) 1386 WP_001025168.1 type II secretion system protein GspE Machinery gene
  HR073_RS03185 (HR073_03180) pilA 691790..692230 (-) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  HR073_RS03190 (HR073_03185) nadC 692433..693326 (-) 894 WP_001135167.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HR073_RS03195 (HR073_03190) ampD 693414..693965 (+) 552 WP_000923726.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HR073_RS03200 (HR073_03195) ampE 693962..694816 (+) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  HR073_RS03205 (HR073_03200) aroP 694859..696232 (-) 1374 WP_000969912.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50614.28 Da        Isoelectric Point: 6.5405

>NTDB_id=398304 HR073_RS03180 WP_001025168.1 690395..691780(-) (pilB) [Escherichia coli strain EcPF15]
MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAIHEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDVGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLVLVTGPTGSGKTVTLYSALQTLN
TADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TTETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRLQQGESIHIPDNVWSSPLPRWQAPGCVHCYHGFYGRTALFE
VLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=398304 HR073_RS03180 WP_001025168.1 690395..691780(-) (pilB) [Escherichia coli strain EcPF15]
ATGAATATTCCACAGCTCACCGCCCTGTGTCTGCGTTATCAGGGAGTCTTGCTGGATGCCAGCGAAGAGGTGGTTCATGT
TGCGGTGGTCGATGCCCCTTCGCATGAGCTACTAGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTATTCATGAGAAGCATCAGCCC
AAAGCAGAGTTGCTGACTCGAACGTTACAATCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGATGTTTCACCGGATGTCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGCAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTACCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGAGTCAGGCACTGGATGTCAACACGCTTGGAATGCAGCCGTTACAACTGGCGGACTTTGCTCATGCCTTGCAACAAC
CACAGGGACTGGTGCTGGTAACAGGCCCTACAGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAACGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCGGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATCC
GCGTGCCGGACTCACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGACCCTGACGTCATTATGATCGGAGAGATCC
GCGATGGCGAAACAGCAGAGATCGCTATTAAAGCGGCGCAAACCGGTCACCTGGTGTTGTCTACCCTACACACCAATTCC
ACCACTGAAACGCTGGTACGTTTACAGCAAATGGGAGTCGCCCGCTGGATGCTCTCATCAGCGCTTACACTGGTAATAGC
CCAGCGTCTGGTACGCAAACTTTGCCCACATTGTCGCCTGCAGCAAGGGGAGTCCATCCATATTCCAGACAATGTATGGT
CGTCGCCGCTGCCCCGCTGGCAAGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGTACGGCCTTATTTGAA
GTTCTACCCATAACACCGGTCATACGCCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACGCACGCCCGACA
GGCGGGTATGCGTACGCTTTTTGAAAACGGCTGCCTGGCCGTGGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TATTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(88-353)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

41.469

100

0.416
  pilB Legionella pneumophila strain ERS1305867

49.738

82.863

0.412
  pilB Haemophilus influenzae 86-028NP

40.127

100

0.41
  pilB Vibrio campbellii strain DS40M4

48.052

83.514

0.401
  pilB Vibrio parahaemolyticus RIMD 2210633

46.667

84.599

0.395
  pilB Vibrio cholerae strain A1552

47.15

83.731

0.395
  pilF Neisseria gonorrhoeae MS11

44.56

83.731

0.373
  pilB Acinetobacter baylyi ADP1

44.301

83.731

0.371
  pilB Haemophilus influenzae Rd KW20

44.186

83.948

0.371
  pilB Acinetobacter baumannii D1279779

44.125

83.08

0.367

Multiple sequence alignment