Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   VVMO6_RS02910 Genome accession   NC_014965
Coordinates   584820..586508 (-) Length   562 a.a.
NCBI ID   WP_013571140.1    Uniprot ID   -
Organism   Vibrio vulnificus MO6-24/O     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 579820..591508
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VVMO6_RS02880 (VVM_01015) rplS 580356..580709 (+) 354 WP_011079544.1 50S ribosomal protein L19 -
  VVMO6_RS02885 (VVM_01017) yacG 580943..581137 (-) 195 WP_026060801.1 DNA gyrase inhibitor YacG -
  VVMO6_RS02890 (VVM_01020) zapD 581200..581940 (-) 741 WP_011079546.1 cell division protein ZapD -
  VVMO6_RS02895 (VVM_01021) coaE 581973..582581 (-) 609 WP_011151132.1 dephospho-CoA kinase -
  VVMO6_RS02900 (VVM_01024) pilD 582583..583452 (-) 870 WP_013571138.1 A24 family peptidase Machinery gene
  VVMO6_RS02905 (VVM_01026) pilC 583546..584772 (-) 1227 WP_013571139.1 type II secretion system F family protein Machinery gene
  VVMO6_RS02910 (VVM_01027) pilB 584820..586508 (-) 1689 WP_013571140.1 type IV-A pilus assembly ATPase PilB Machinery gene
  VVMO6_RS02915 (VVM_01028) - 586508..586951 (-) 444 WP_013571141.1 pilin -
  VVMO6_RS02920 (VVM_01030) nadC 587174..588061 (-) 888 WP_013571142.1 carboxylating nicotinate-nucleotide diphosphorylase -
  VVMO6_RS02925 (VVM_01031) ampD 588154..588726 (+) 573 WP_013571143.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  VVMO6_RS02930 (VVM_01033) pdhR 589115..589882 (+) 768 WP_011079554.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62434.44 Da        Isoelectric Point: 5.5330

>NTDB_id=39674 VVMO6_RS02910 WP_013571140.1 584820..586508(-) (pilB) [Vibrio vulnificus MO6-24/O]
MMSNLSTILRQANQLSLTQEQACRETIQASGVTAPEALLQLGFFQPDELTEQLSAIFGLPRTDLNQYDYQDLCNQLGLRE
LITRHQALPLQKSNAGLILAVADPTNIQAEDDFRFATGVQVEIVLADCQELQAAIRRLYGKSIKQSQGSGLKEIHQDELA
NLVEVSDDEVESIEDLSQDESPVSRFINQILLDAVRKGASDIHFEPYENQYRVRLRCDGILVEVQQPPSHLSRRLSARIK
ILSKLDIAERRLPQDGRIKLRLNQETAIDMRVSTLPTLFGEKIVLRLLDSSSSSLDIDKLGYNPQQKQLYLDALRKPQGM
ILMTGPTGSGKTVSLYTGLSLLNRPEVNISTAEDPVEINLPGINQVQVKPKIGFGFAEALRSFLRQDPDIVMVGEIRDLE
TAEIAVKAAQTGHLVLSTLHTNSAAETIVRLSNMGIASFNLASSLSLIIAQRLARRLCSHCKQPHPYNATFELLGLKADD
LIYQADRQGCHHCTHGYSGRVGIYEVMPFDEQLAEAIVKGASVQSLEMLARQKGMMTLKDSGLEKLKQGITSLEELQRVL
YL

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=39674 VVMO6_RS02910 WP_013571140.1 584820..586508(-) (pilB) [Vibrio vulnificus MO6-24/O]
ATGATGTCGAATCTCTCCACTATTCTTCGTCAGGCCAATCAACTTAGCCTAACGCAAGAACAAGCGTGTCGAGAGACAAT
ACAAGCTTCTGGTGTGACAGCACCGGAAGCTTTGTTGCAACTGGGATTTTTCCAGCCGGATGAATTAACCGAGCAGCTCA
GCGCGATTTTTGGCTTACCACGCACTGACCTCAACCAATATGACTACCAAGATCTGTGTAATCAACTTGGGTTACGTGAG
CTGATCACTCGTCACCAAGCACTGCCACTGCAAAAAAGCAACGCTGGTTTAATACTCGCGGTTGCTGACCCTACCAATAT
TCAAGCTGAAGATGATTTCCGTTTTGCCACAGGTGTGCAGGTGGAGATTGTTTTGGCTGATTGCCAAGAGCTGCAAGCAG
CGATTCGTAGACTCTATGGAAAGAGCATCAAACAGAGCCAAGGCTCTGGTTTAAAAGAGATTCACCAAGATGAGCTGGCG
AATTTAGTCGAGGTTTCAGATGACGAAGTCGAGTCAATTGAAGACTTAAGCCAAGATGAATCTCCCGTTAGCCGCTTTAT
TAACCAAATTCTGCTGGATGCGGTACGCAAAGGCGCATCTGATATTCATTTTGAACCGTACGAAAACCAATACCGCGTGC
GATTGCGTTGCGATGGTATTTTGGTGGAAGTGCAGCAGCCTCCAAGCCATTTAAGCCGCCGTTTATCTGCGAGGATAAAG
ATCCTCTCCAAGCTGGATATTGCGGAAAGGCGTCTGCCACAAGATGGACGGATTAAGCTGCGCCTCAACCAAGAAACCGC
GATTGATATGCGCGTTTCCACCTTACCCACACTGTTTGGTGAAAAAATTGTGTTGCGACTTCTAGACAGCTCGAGCAGTA
GCTTGGATATTGATAAGTTAGGCTACAACCCGCAACAAAAACAGCTCTACCTCGATGCCCTGCGCAAACCGCAAGGGATG
ATTTTGATGACGGGCCCAACAGGAAGCGGCAAAACCGTTTCGCTGTACACAGGCTTGAGTTTACTTAACCGGCCAGAAGT
GAATATCTCCACCGCGGAAGACCCAGTCGAGATTAACCTCCCGGGAATCAACCAAGTGCAAGTGAAACCCAAAATTGGTT
TTGGTTTTGCAGAAGCATTGCGTTCTTTTCTCCGTCAAGACCCGGATATCGTCATGGTGGGGGAAATTCGAGATTTGGAA
ACCGCCGAGATCGCCGTCAAAGCAGCCCAAACCGGTCACCTTGTGCTATCCACCTTACATACCAACTCGGCTGCTGAAAC
CATTGTTCGTCTATCTAACATGGGGATCGCCAGCTTTAACTTGGCTTCATCCTTGAGTTTGATCATTGCTCAGCGTCTGG
CTCGTCGGTTATGCAGCCATTGCAAGCAACCTCATCCATACAACGCAACATTTGAGTTACTCGGTCTAAAAGCTGACGAC
TTGATTTATCAAGCTGATAGACAGGGTTGCCATCATTGTACTCATGGTTATTCCGGTCGTGTCGGTATATACGAAGTCAT
GCCTTTTGATGAACAGCTTGCAGAGGCGATCGTTAAAGGCGCCTCGGTACAAAGCTTAGAGATGCTTGCTCGACAAAAAG
GCATGATGACACTCAAAGATTCCGGCCTTGAAAAACTCAAACAGGGCATCACCAGCTTAGAAGAGCTGCAACGCGTCCTC
TATTTGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-141)

Type II/IV secretion system protein (T2SSE)

(189-456)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

76.69

100

0.767
  pilB Vibrio parahaemolyticus RIMD 2210633

76.157

100

0.762
  pilB Vibrio cholerae strain A1552

74.423

100

0.746
  pilB Legionella pneumophila strain ERS1305867

53.216

91.281

0.486
  pilB Acinetobacter baumannii D1279779

51.538

92.527

0.477
  pilB Acinetobacter baylyi ADP1

50.478

93.06

0.47
  pilF Neisseria gonorrhoeae MS11

45.31

100

0.456
  pilF Thermus thermophilus HB27

41.429

99.644

0.413
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.491

94.306

0.363

Multiple sequence alignment