Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   GH742_RS10600 Genome accession   NZ_CP045732
Coordinates   2294593..2295657 (-) Length   354 a.a.
NCBI ID   WP_203454937.1    Uniprot ID   -
Organism   Legionella sp. MW5194     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2289593..2300657
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GH742_RS10575 (GH742_10525) aroK 2290077..2290604 (-) 528 WP_058527208.1 shikimate kinase AroK -
  GH742_RS10580 (GH742_10530) pilQ 2290676..2292811 (-) 2136 WP_203454933.1 type IV pilus secretin PilQ Machinery gene
  GH742_RS10585 (GH742_10535) pilP 2292821..2293345 (-) 525 WP_203454934.1 pilus assembly protein PilP Machinery gene
  GH742_RS10590 (GH742_10540) pilO 2293342..2293959 (-) 618 WP_203454935.1 type 4a pilus biogenesis protein PilO Machinery gene
  GH742_RS10595 (GH742_10545) pilN 2293956..2294588 (-) 633 WP_203454936.1 PilN domain-containing protein Machinery gene
  GH742_RS10600 (GH742_10550) pilM 2294593..2295657 (-) 1065 WP_203454937.1 pilus assembly protein PilM Machinery gene
  GH742_RS10605 (GH742_10555) - 2296023..2297237 (+) 1215 WP_203454938.1 hypothetical protein -
  GH742_RS10610 (GH742_10560) - 2297364..2299739 (+) 2376 WP_203454939.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38567.44 Da        Isoelectric Point: 4.8710

>NTDB_id=396446 GH742_RS10600 WP_203454937.1 2294593..2295657(-) (pilM) [Legionella sp. MW5194]
MLKLFKPKSRSIIGIDITSSSVKVLEISSADNKYRIEGYGAEPLPANAIEGNTVKDVEAVAMCIKRVLSKAHLSGKHAVV
AVPDSAVISKVVQINDGLNDAEIEELIVMEADKYIPYPIDEINIDFEVLGHSPKNSAMLDVLIVASRAENVGSRVDAVNR
AGLEAKIVDVESFAVERAAHLLAKELPAQGQDKIVAVIDIGALYTHLFVLHGMKIIFTREEEFGGKQLIDSIASQYQVSF
EQAALMKAEGNLPENYEEEVLEPFIEMILLQVKRTLQFFFSTSHHGFVDHIILAGGVARLPNLASLVQEKTGIPTSVADP
LHEMDFGKYVNRDEVISQAPSLLVACGLALRTVG

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=396446 GH742_RS10600 WP_203454937.1 2294593..2295657(-) (pilM) [Legionella sp. MW5194]
GTGCTCAAACTGTTTAAACCTAAAAGCCGCTCAATCATCGGGATTGATATTACTTCTTCCTCTGTTAAAGTATTGGAAAT
TTCCAGCGCTGATAATAAATACCGCATTGAAGGCTATGGGGCTGAGCCTTTGCCCGCCAATGCCATTGAAGGCAACACGG
TCAAAGACGTTGAGGCAGTGGCCATGTGCATCAAACGCGTTTTATCCAAAGCCCACTTATCCGGCAAACACGCCGTGGTG
GCGGTACCTGATTCAGCTGTTATCAGCAAGGTTGTTCAGATTAACGATGGATTGAACGATGCCGAGATCGAAGAACTCAT
CGTCATGGAGGCTGATAAATACATCCCTTATCCTATCGATGAAATCAACATTGATTTTGAAGTACTGGGGCATTCGCCTA
AAAATTCCGCCATGCTTGATGTTCTCATTGTAGCCTCTCGGGCGGAGAATGTGGGCAGCCGTGTCGATGCGGTTAACCGC
GCCGGGCTTGAGGCTAAAATTGTCGACGTGGAGTCCTTTGCTGTGGAACGGGCCGCGCATTTGCTGGCAAAAGAATTACC
GGCCCAGGGACAGGATAAAATTGTCGCCGTCATTGACATTGGCGCTTTATATACTCACCTGTTTGTCCTGCACGGCATGA
AAATCATCTTTACCCGGGAGGAGGAGTTCGGCGGCAAGCAGTTAATTGATTCCATCGCCAGTCAGTATCAGGTCAGTTTT
GAACAGGCTGCCCTGATGAAAGCCGAGGGGAATCTGCCTGAGAATTATGAGGAAGAAGTGCTTGAACCCTTTATCGAGAT
GATTCTGTTGCAGGTGAAACGCACCCTGCAGTTTTTCTTCTCTACCAGTCACCATGGTTTTGTCGATCATATCATTTTAG
CTGGCGGTGTCGCCAGACTGCCTAATCTGGCAAGCCTTGTGCAGGAAAAAACCGGAATCCCGACCAGCGTGGCCGACCCA
CTGCATGAAATGGATTTTGGTAAATACGTCAATCGCGACGAAGTGATAAGTCAGGCGCCTTCGTTACTGGTAGCCTGTGG
TCTTGCATTGAGAACAGTAGGGTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-352)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Legionella pneumophila strain ERS1305867

66.289

99.718

0.661
  pilM Acinetobacter baumannii D1279779

43.182

99.435

0.429
  comM Acinetobacter nosocomialis M2

42.898

99.435

0.427
  comM Acinetobacter baylyi ADP1

42.614

99.435

0.424

Multiple sequence alignment