Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   GH742_RS10580 Genome accession   NZ_CP045732
Coordinates   2290676..2292811 (-) Length   711 a.a.
NCBI ID   WP_203454933.1    Uniprot ID   -
Organism   Legionella sp. MW5194     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2285676..2297811
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GH742_RS10555 (GH742_10505) - 2286319..2286753 (-) 435 WP_203454929.1 universal stress protein -
  GH742_RS10560 (GH742_10510) pnuC 2286928..2287524 (+) 597 WP_203454930.1 nicotinamide riboside transporter PnuC -
  GH742_RS10565 (GH742_10515) - 2287507..2288979 (-) 1473 WP_203454931.1 AAA family ATPase -
  GH742_RS10570 (GH742_10520) aroB 2288963..2290090 (-) 1128 WP_203454932.1 3-dehydroquinate synthase -
  GH742_RS10575 (GH742_10525) aroK 2290077..2290604 (-) 528 WP_058527208.1 shikimate kinase AroK -
  GH742_RS10580 (GH742_10530) pilQ 2290676..2292811 (-) 2136 WP_203454933.1 type IV pilus secretin PilQ Machinery gene
  GH742_RS10585 (GH742_10535) pilP 2292821..2293345 (-) 525 WP_203454934.1 pilus assembly protein PilP Machinery gene
  GH742_RS10590 (GH742_10540) pilO 2293342..2293959 (-) 618 WP_203454935.1 type 4a pilus biogenesis protein PilO Machinery gene
  GH742_RS10595 (GH742_10545) pilN 2293956..2294588 (-) 633 WP_203454936.1 PilN domain-containing protein Machinery gene
  GH742_RS10600 (GH742_10550) pilM 2294593..2295657 (-) 1065 WP_203454937.1 pilus assembly protein PilM Machinery gene
  GH742_RS10605 (GH742_10555) - 2296023..2297237 (+) 1215 WP_203454938.1 hypothetical protein -

Sequence

Protein


Download         Length: 711 a.a.        Molecular weight: 77967.77 Da        Isoelectric Point: 9.8055

>NTDB_id=396442 GH742_RS10580 WP_203454933.1 2290676..2292811(-) (pilQ) [Legionella sp. MW5194]
MRKIITALLLTFCLGLAYAKDNALVGIKTIPLPDNRVRLDFQFAEPVKQMPASFITQKPSRLILDFIHSANQLNPSMQTR
KIDIGSLTDYKVVSVGDRVRAILNLDGSVAYSGYVNGNVYSLTLSGKSNQLFQERKEIFVSNRASSNTKHRINNIDFRGT
NKGGRVVVSVSDSSIPIDVTQTGREIVVNFSSTSVPPQLAKRYDVRDFQSPAQVISVQQEGKNVRMTLTSKGDYGHFAYQ
VNKQFMVDVFPLTEEEIKQAKLKKEVFVGKRISLNFQNIPVRSVLQLLAEFTGINIVVSDKVTNNITLRLNDIPWDQALD
IILKTNGLDKRRTGNIMMIAPSEDLLKREKEELKAQQEVTSLAPLRSELLQINYAKAADIATLLKDKNTSLLSKRGNLSV
DARTNTIWIQDTGLQIEEIRELVKQLDIPVKQVLIEARIVNVTKDFARDLGIRFGVSRPTHLSGTLSGANQMEQGIAPAD
VTPLADRLNLDLAAVPVSASPASIGIALAKLGDGILLDLELSALESEGRGEVIASPRLITTNQQAAVIESGEEIPYQEAT
SSGATAVAFKKAVLSLKVTPQITPDSKIMMDLQINQDTPSAQTFNGVPSILTKEIQTNVLVNNGQTIVLGGIYKQDKNNA
INRVPFLGELPVVGALFRNTSTSIKNEELLIFITPRIITNTLSITTVEGRGKAVAKGCELDKFGKPVGPFK

Nucleotide


Download         Length: 2136 bp        

>NTDB_id=396442 GH742_RS10580 WP_203454933.1 2290676..2292811(-) (pilQ) [Legionella sp. MW5194]
TTGCGTAAGATTATTACAGCGTTATTATTGACTTTTTGCCTGGGGCTGGCTTATGCCAAGGATAACGCGCTGGTAGGGAT
TAAGACCATCCCTTTACCGGATAACCGGGTGCGCCTTGATTTCCAGTTTGCCGAGCCAGTCAAACAGATGCCCGCGAGCT
TTATTACCCAAAAGCCGTCGCGGCTCATTCTGGATTTTATTCACAGCGCCAATCAACTGAATCCCTCCATGCAAACCAGG
AAAATCGACATTGGTTCTCTAACCGATTATAAAGTGGTCTCCGTGGGTGATCGTGTCCGGGCTATTCTTAACCTTGATGG
CTCGGTCGCCTATTCAGGGTATGTCAATGGCAATGTCTACTCCCTGACCTTAAGCGGCAAGAGTAACCAGCTCTTTCAGG
AGCGAAAAGAAATTTTTGTCAGTAACCGGGCATCATCCAATACCAAACACCGCATTAACAACATTGATTTCCGCGGCACC
AACAAAGGCGGGCGCGTGGTGGTCAGTGTGTCTGACAGCAGTATCCCCATCGATGTCACCCAGACAGGGCGTGAAATTGT
GGTTAATTTCTCAAGCACCAGCGTACCGCCGCAATTGGCCAAGCGCTACGATGTGCGTGATTTCCAAAGCCCGGCGCAAG
TGATTTCGGTGCAGCAGGAAGGAAAAAATGTCCGTATGACACTGACGTCAAAAGGAGATTATGGGCATTTCGCCTATCAG
GTGAATAAGCAGTTCATGGTGGATGTCTTCCCGCTGACTGAGGAAGAAATCAAACAGGCAAAATTGAAGAAAGAAGTGTT
TGTTGGTAAACGCATCTCCTTAAACTTCCAGAATATTCCAGTGCGTTCAGTCCTGCAGTTGCTGGCTGAATTTACCGGCA
TTAACATTGTGGTCAGCGATAAAGTCACCAATAACATTACCCTGCGCCTGAATGATATTCCCTGGGATCAGGCACTGGAT
ATTATCCTTAAAACCAATGGTCTTGATAAACGCAGGACCGGTAACATCATGATGATTGCCCCATCGGAGGATTTGCTCAA
GCGCGAAAAGGAAGAGCTTAAAGCCCAGCAGGAAGTCACAAGCTTGGCGCCCCTTCGTTCCGAGCTGCTGCAAATCAATT
ACGCGAAAGCGGCGGACATTGCCACCCTGCTAAAGGATAAAAACACGTCGCTGTTATCCAAACGCGGCAATTTGAGTGTG
GATGCACGCACCAATACCATCTGGATTCAGGACACGGGTCTTCAGATTGAAGAAATCCGCGAACTGGTCAAACAATTGGA
TATCCCTGTCAAACAGGTGCTCATTGAAGCTCGCATTGTGAACGTGACCAAGGATTTCGCCAGGGATTTGGGCATTCGCT
TCGGTGTTTCAAGACCGACGCATTTAAGCGGTACCTTAAGCGGTGCCAATCAGATGGAGCAGGGGATTGCGCCAGCCGAT
GTAACACCATTGGCGGATCGTTTAAACCTTGATTTGGCCGCTGTTCCGGTATCCGCCTCACCCGCCTCCATTGGTATTGC
TTTAGCCAAGCTTGGCGACGGCATCCTGCTTGATCTGGAACTTTCGGCGCTGGAAAGTGAAGGACGGGGAGAGGTCATCG
CCAGCCCGCGATTAATTACCACCAACCAGCAGGCTGCGGTGATTGAATCAGGGGAAGAAATTCCTTACCAGGAAGCCACC
TCAAGCGGCGCCACGGCCGTGGCCTTCAAAAAGGCAGTATTGAGTCTTAAGGTGACTCCCCAGATAACCCCAGACAGTAA
AATCATGATGGATTTGCAGATTAATCAGGATACCCCTTCCGCTCAAACCTTTAATGGCGTACCCTCCATCCTGACCAAAG
AGATACAGACCAATGTGTTGGTGAATAACGGCCAAACCATTGTTTTGGGGGGAATTTACAAACAGGATAAAAACAATGCA
ATTAATCGCGTGCCTTTCCTGGGAGAATTGCCCGTGGTTGGTGCACTATTCCGAAATACATCCACATCAATTAAAAATGA
GGAGTTGCTCATTTTCATTACTCCTAGGATAATAACAAACACCTTGTCCATTACCACCGTTGAAGGACGGGGGAAAGCGG
TTGCCAAGGGTTGTGAGTTGGATAAATTCGGGAAGCCTGTCGGGCCTTTTAAATAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(369-432)

Secretin and TonB N terminus short domain (STN)

(294-341)

AMIN domain (AMIN)

(42-112)

Bacterial type II and III secretion system protein (Secretin)

(523-678)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Legionella pneumophila strain ERS1305867

73.715

95.781

0.706
  pilQ Legionella pneumophila strain Lp02

73.353

96.062

0.705
  comQ Acinetobacter baylyi ADP1

40.288

97.75

0.394
  pilQ Acinetobacter baumannii strain A118

38.516

98.594

0.38
  pilQ Acinetobacter baumannii D1279779

38.516

98.594

0.38
  pilQ Pseudomonas aeruginosa PAK

38.971

95.64

0.373

Multiple sequence alignment