Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   GH742_RS10590 Genome accession   NZ_CP045732
Coordinates   2293342..2293959 (-) Length   205 a.a.
NCBI ID   WP_203454935.1    Uniprot ID   -
Organism   Legionella sp. MW5194     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2288342..2298959
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GH742_RS10570 (GH742_10520) aroB 2288963..2290090 (-) 1128 WP_203454932.1 3-dehydroquinate synthase -
  GH742_RS10575 (GH742_10525) aroK 2290077..2290604 (-) 528 WP_058527208.1 shikimate kinase AroK -
  GH742_RS10580 (GH742_10530) pilQ 2290676..2292811 (-) 2136 WP_203454933.1 type IV pilus secretin PilQ Machinery gene
  GH742_RS10585 (GH742_10535) pilP 2292821..2293345 (-) 525 WP_203454934.1 pilus assembly protein PilP Machinery gene
  GH742_RS10590 (GH742_10540) pilO 2293342..2293959 (-) 618 WP_203454935.1 type 4a pilus biogenesis protein PilO Machinery gene
  GH742_RS10595 (GH742_10545) pilN 2293956..2294588 (-) 633 WP_203454936.1 PilN domain-containing protein Machinery gene
  GH742_RS10600 (GH742_10550) pilM 2294593..2295657 (-) 1065 WP_203454937.1 pilus assembly protein PilM Machinery gene
  GH742_RS10605 (GH742_10555) - 2296023..2297237 (+) 1215 WP_203454938.1 hypothetical protein -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 23367.04 Da        Isoelectric Point: 6.0990

>NTDB_id=396444 GH742_RS10590 WP_203454935.1 2293342..2293959(-) (pilO) [Legionella sp. MW5194]
MNNFNLNELTFDNVGQWPAPVKYFMAGLLALILIGIGYWVIVKPNFEQHDALLAQQATLKTEFEQKQQQAVNLQAYRVQL
QIMNERFGNMLKQLPAQNEMPGLLEDISKTGISSGLTFELFAPQPEVVHDFYVELPINITVVGNYHQLALFLSRIAEMSR
IVTLHDFEIANLSDKNKQQPGAKAVTNPDLLEMKITAKIYRYRTL

Nucleotide


Download         Length: 618 bp        

>NTDB_id=396444 GH742_RS10590 WP_203454935.1 2293342..2293959(-) (pilO) [Legionella sp. MW5194]
ATGAACAATTTCAATCTCAATGAGTTAACGTTTGATAACGTGGGCCAGTGGCCGGCGCCAGTGAAGTATTTTATGGCGGG
CTTACTCGCGCTCATTCTGATTGGTATTGGCTACTGGGTGATCGTAAAGCCAAATTTTGAACAACACGATGCCTTGCTCG
CTCAGCAAGCCACGCTAAAAACCGAATTTGAGCAAAAACAGCAGCAAGCCGTAAATTTGCAGGCTTATCGTGTCCAGCTT
CAGATTATGAATGAGCGTTTTGGCAACATGCTTAAGCAATTACCGGCACAAAACGAAATGCCTGGGCTGCTCGAAGACAT
CTCCAAAACCGGTATTTCAAGCGGTTTGACGTTTGAGCTTTTCGCACCGCAACCCGAGGTGGTTCATGATTTCTACGTCG
AGTTGCCTATCAATATTACGGTGGTTGGCAATTATCATCAACTGGCCCTGTTTTTAAGCCGAATTGCCGAGATGAGCCGC
ATTGTCACCTTGCATGATTTTGAAATTGCGAATCTTTCTGATAAAAATAAACAACAGCCAGGCGCAAAGGCTGTGACCAA
TCCTGATCTTTTGGAAATGAAAATTACAGCGAAAATATATCGGTACAGGACATTATGA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(54-203)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

66.667

99.512

0.663
  comO Acinetobacter baylyi ADP1

38.071

96.098

0.366

Multiple sequence alignment