Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   GFU70_RS02355 Genome accession   NZ_CP045701
Coordinates   533940..536027 (+) Length   695 a.a.
NCBI ID   WP_153387540.1    Uniprot ID   -
Organism   Pseudomonas brassicacearum strain S-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 528940..541027
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GFU70_RS02335 (GFU70_02335) comM 531151..532215 (+) 1065 WP_058542652.1 pilus assembly protein PilM Machinery gene
  GFU70_RS02340 (GFU70_02340) - 532215..532781 (+) 567 WP_058542653.1 PilN domain-containing protein -
  GFU70_RS02345 (GFU70_02345) pilO 532778..533401 (+) 624 WP_116643335.1 type 4a pilus biogenesis protein PilO -
  GFU70_RS02350 (GFU70_02350) - 533398..533943 (+) 546 WP_116643334.1 pilus assembly protein PilP -
  GFU70_RS02355 (GFU70_02355) pilQ 533940..536027 (+) 2088 WP_153387540.1 type IV pilus secretin PilQ Machinery gene
  GFU70_RS02360 (GFU70_02360) aroK 536032..536550 (+) 519 WP_003196983.1 shikimate kinase AroK -
  GFU70_RS02365 (GFU70_02365) aroB 536699..537799 (+) 1101 WP_116643332.1 3-dehydroquinate synthase -
  GFU70_RS02370 (GFU70_02370) - 537809..539368 (+) 1560 WP_153387541.1 AAA family ATPase -

Sequence

Protein


Download         Length: 695 a.a.        Molecular weight: 74853.86 Da        Isoelectric Point: 5.2856

>NTDB_id=396095 GFU70_RS02355 WP_153387540.1 533940..536027(+) (pilQ) [Pseudomonas brassicacearum strain S-1]
MNRIFPALGISLWIALLSPMVHGASLKTLDVAALPGDRVELKLTFDGPPPTPRGYTTEQPARIALDLPGVTNQLASKRHD
LGGGNARSATVVEAGDRTRLIIGLTQLAPYDARVEGNNLFVVVGKGAASTGTAPKSAAQASRPAVASAAPARASAPVGKA
IRGVDFQRGTQGEGNVVIDLSDPSITPDIQEREGKIILNFARTQLPEPLRVRLDVKDFATPVQFVNASAGSERATISIEP
SGTFDYSTYQTDNKLTVSVRPMTVDDLQKRNADRAAYTGEKLSLNFQDIEVRSVLQLIADFTNLNLVASDTVQGGITLRL
QNVPWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIADLAPLRRELLQVNYAKAADIAKLFQSVTSA
EAKADERGTITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSVQNKGNWSSSG
VTTGDSTTIGTPGSTSTNQPFVDLGASGNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTE
IPYQEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVNDVPPIKKNEVNAKVLVNDGETIVIGGV
FSNTQSKVVDKVPFLGDVPYLGRLFRRDVVSDSKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2088 bp        

>NTDB_id=396095 GFU70_RS02355 WP_153387540.1 533940..536027(+) (pilQ) [Pseudomonas brassicacearum strain S-1]
ATGAACAGGATTTTTCCAGCCCTCGGTATTTCGCTATGGATAGCGCTGTTGTCGCCGATGGTTCATGGGGCCAGTCTGAA
GACGCTGGATGTGGCGGCGTTGCCGGGGGACCGTGTCGAGTTGAAGCTGACCTTCGACGGGCCGCCGCCGACGCCCCGGG
GCTACACCACCGAACAACCGGCGCGGATTGCACTGGATCTGCCGGGCGTGACCAATCAACTGGCCAGCAAGCGCCACGAC
CTGGGCGGGGGCAATGCACGTAGCGCTACCGTGGTCGAGGCTGGGGATCGCACCCGGTTGATCATCGGTCTTACCCAGCT
GGCGCCCTATGACGCCCGGGTCGAAGGTAACAACCTGTTCGTGGTGGTTGGCAAGGGCGCCGCCTCGACGGGCACTGCAC
CGAAGAGCGCGGCCCAGGCCTCGCGCCCGGCGGTTGCCAGCGCTGCGCCTGCGAGGGCCAGTGCGCCGGTGGGCAAGGCG
ATCCGTGGAGTGGACTTCCAGCGCGGGACCCAGGGCGAAGGTAACGTGGTGATCGATCTGTCTGACCCGTCCATCACCCC
GGACATCCAGGAGCGCGAAGGCAAGATCATCCTCAATTTCGCCAGGACCCAATTGCCCGAGCCACTGCGGGTGCGCCTGG
ACGTCAAGGACTTCGCCACCCCGGTGCAATTCGTCAATGCCAGTGCCGGCAGTGAGCGGGCGACCATCAGCATCGAACCC
AGCGGCACCTTCGACTACTCGACCTACCAGACCGACAATAAGCTCACCGTCAGCGTCCGGCCCATGACCGTCGACGACCT
GCAAAAACGCAACGCCGACCGCGCGGCCTACACTGGCGAGAAGCTCTCGCTCAACTTCCAGGACATCGAGGTGCGGTCGG
TCCTGCAACTGATTGCCGATTTTACCAACCTCAACCTCGTGGCCAGCGACACAGTGCAAGGAGGCATCACCCTGCGGTTG
CAGAATGTGCCCTGGGACCAGGCGCTCGACCTGGTGCTCAAGACCAAGGGGTTGGACAAGCGCAAGGTCGGCAACGTGCT
GTTGGTGGCGCCGGCCGATGAAATTGCCGCCCGTGAGCGTCAGGAACTGGAGTCGCAGAAGCAGATCGCCGACCTGGCAC
CGCTGCGGCGTGAACTGTTGCAGGTCAACTATGCGAAGGCGGCCGACATCGCCAAGTTGTTCCAATCGGTGACCAGTGCC
GAGGCGAAAGCCGATGAGCGCGGCACGATCACGGTCGATGAGCGCACCAACAACATCATTGCCTACCAGACCCAGGACCG
CCTCGACGAACTGCGCCGGATCGTGGCGCAGCTGGATATTCCAGTGCGCCAGGTGATGATCGAGGCGCGGATTGTCGAGG
CCAACGTCGACTACGACAAAAGCCTGGGCGTACGCTGGGGCGGTTCGGTTCAAAACAAGGGCAACTGGAGCTCCTCGGGG
GTCACCACGGGCGACTCCACCACTATCGGCACGCCGGGCAGCACCAGCACCAACCAGCCGTTCGTCGACCTGGGCGCCTC
GGGCAACACCTCGGGCATCGGCATCGCCTTCATCACCGACAACGTATTGCTGGACCTTGAGCTGACGGCCATGGAGAAAA
CCGGTAATGGGGAGATTGTCTCCCAGCCCAAGGTGGTGACATCCGACAAGGAGACCGCAAAGATCCTCAAGGGCACGGAA
ATTCCTTATCAGGAAGCCAGCTCCAGCGGCGCTACGTCGGTATCGTTCAAGGAGGCTTCGCTATCGCTGGAGGTGACCCC
GCAGATCACCCCAGACAACCGGATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCGGACTACCTGAACAAGGTGAATG
ATGTGCCGCCGATCAAGAAAAACGAGGTCAATGCCAAGGTCCTGGTCAATGATGGCGAGACCATTGTGATTGGTGGGGTT
TTCTCAAATACTCAGAGCAAGGTTGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCG
TGATGTGGTTTCGGATAGCAAATCCGAGCTGCTGGTGTTCCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGA
GTCGTTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(38-122)

Bacterial type II/III secretion system short domain (Secretin_N)

(379-444)

Bacterial type II and III secretion system protein (Secretin)

(530-686)

Secretin and TonB N terminus short domain (STN)

(305-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.867

100

0.75
  comQ Acinetobacter baylyi ADP1

41.439

100

0.414
  pilQ Acinetobacter baumannii D1279779

38.121

100

0.409
  pilQ Acinetobacter baumannii strain A118

38.121

100

0.409
  pilQ Legionella pneumophila strain Lp02

38.382

97.842

0.376
  pilQ Legionella pneumophila strain ERS1305867

38.382

97.842

0.376

Multiple sequence alignment