Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   GFU70_RS02335 Genome accession   NZ_CP045701
Coordinates   531151..532215 (+) Length   354 a.a.
NCBI ID   WP_058542652.1    Uniprot ID   -
Organism   Pseudomonas brassicacearum strain S-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 526151..537215
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GFU70_RS02325 (GFU70_02325) - 526975..528243 (+) 1269 WP_058542650.1 malic enzyme-like NAD(P)-binding protein -
  GFU70_RS02330 (GFU70_02330) - 528499..530943 (-) 2445 WP_165826103.1 penicillin-binding protein 1A -
  GFU70_RS02335 (GFU70_02335) comM 531151..532215 (+) 1065 WP_058542652.1 pilus assembly protein PilM Machinery gene
  GFU70_RS02340 (GFU70_02340) - 532215..532781 (+) 567 WP_058542653.1 PilN domain-containing protein -
  GFU70_RS02345 (GFU70_02345) pilO 532778..533401 (+) 624 WP_116643335.1 type 4a pilus biogenesis protein PilO -
  GFU70_RS02350 (GFU70_02350) - 533398..533943 (+) 546 WP_116643334.1 pilus assembly protein PilP -
  GFU70_RS02355 (GFU70_02355) pilQ 533940..536027 (+) 2088 WP_153387540.1 type IV pilus secretin PilQ Machinery gene
  GFU70_RS02360 (GFU70_02360) aroK 536032..536550 (+) 519 WP_003196983.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38127.75 Da        Isoelectric Point: 4.9112

>NTDB_id=396094 GFU70_RS02335 WP_058542652.1 531151..532215(+) (comM) [Pseudomonas brassicacearum strain S-1]
MLRLFNKKAHALLGIDISSTSVKLLELSRQGERYRVESYAVEPLPVNAVVEKNIAELEGVGQALSRLLAKAKTPLRSVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKIEADQYIPYPLDEVAIDFEVVGVSPRSTERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERAFGMLATQLAASQERLTVAVVDIGATMTTLSVLHNGRIIYTREQLFGGRQLTEEIQRRYGLTP
EQAGLAKKQGGLPDDYLSEVLQPFREALVQQVSRSLQFFFASGQYSAVDHILLAGGTASVAGLDRLIEQRLGTPTQVANP
FTNMALSSKVNAGALAGDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=396094 GFU70_RS02335 WP_058542652.1 531151..532215(+) (comM) [Pseudomonas brassicacearum strain S-1]
GTGCTACGACTCTTCAATAAAAAAGCCCATGCGCTTCTGGGGATCGACATCAGTTCGACGTCGGTGAAGCTGCTGGAGTT
GAGCCGCCAGGGCGAGCGATACCGCGTCGAGTCCTACGCGGTCGAACCGCTGCCAGTCAACGCCGTGGTCGAGAAGAACA
TCGCCGAGCTCGAAGGGGTGGGCCAGGCGTTGTCGCGGCTGTTGGCCAAGGCCAAGACGCCCTTGCGTAGCGTGGCGGTG
GCCGTGGCGGGTTCGGCGGTGATCACCAAGACCATCGAGATGGACGCCGGTCTGTCCGACGATGACATGGAAAACCAGCT
CAAGATCGAGGCCGATCAGTACATCCCTTATCCCCTGGATGAGGTGGCCATCGATTTCGAAGTGGTGGGTGTTTCGCCGC
GCAGTACCGAGCGCGTCGAAGTGCTGCTGGCGGCGTGCCGCAAGGAAAACGTCGAGGTCCGCGAGGCCGCGTTGGCGCTC
GCCGGGCTGACGGCGCGGGTGGTCGATGTGGAGGCTTATGCGTTGGAGCGGGCTTTTGGCATGCTCGCCACGCAACTGGC
CGCGTCCCAGGAGCGGCTGACCGTGGCGGTTGTCGACATCGGCGCCACCATGACCACCCTCAGCGTGCTGCACAACGGAC
GCATCATCTATACCCGCGAACAATTGTTCGGTGGCCGCCAGCTGACCGAGGAAATCCAGCGCCGCTATGGCCTGACTCCC
GAGCAGGCCGGCCTGGCAAAGAAGCAGGGTGGCCTGCCGGACGATTATCTCAGCGAAGTGCTGCAACCTTTTCGTGAGGC
CCTGGTACAGCAGGTCTCGCGGTCCCTGCAGTTCTTCTTCGCCTCGGGCCAGTACAGTGCGGTGGACCACATTCTGCTGG
CCGGGGGCACGGCTTCGGTCGCCGGCCTGGATCGGCTGATCGAGCAGCGCTTGGGCACGCCGACCCAGGTGGCCAACCCG
TTTACCAACATGGCCCTGAGCAGCAAGGTCAATGCCGGCGCCCTGGCTGGTGATGCGCCCGCACTGATGATTGCCTGTGG
GCTGGCCCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Acinetobacter nosocomialis M2

55.932

100

0.559
  pilM Acinetobacter baumannii D1279779

55.932

100

0.559
  comM Acinetobacter baylyi ADP1

54.52

100

0.545
  pilM Legionella pneumophila strain ERS1305867

47.458

100

0.475

Multiple sequence alignment