Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GFB47_RS01500 Genome accession   NZ_CP045699
Coordinates   335828..337516 (-) Length   562 a.a.
NCBI ID   WP_153446007.1    Uniprot ID   A0A5Q0TG00
Organism   Vibrio algicola strain SM1977     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 330828..342516
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GFB47_RS01475 (GFB47_01480) yacG 331879..332082 (-) 204 WP_153445999.1 DNA gyrase inhibitor YacG -
  GFB47_RS01480 (GFB47_01485) zapD 332136..332867 (-) 732 WP_407701681.1 cell division protein ZapD -
  GFB47_RS01485 (GFB47_01490) coaE 332979..333590 (-) 612 WP_153446001.1 dephospho-CoA kinase -
  GFB47_RS01490 (GFB47_01495) pilD 333595..334506 (-) 912 WP_153446003.1 A24 family peptidase Machinery gene
  GFB47_RS01495 (GFB47_01500) pilC 334545..335777 (-) 1233 WP_153446005.1 type II secretion system F family protein Machinery gene
  GFB47_RS01500 (GFB47_01505) pilB 335828..337516 (-) 1689 WP_153446007.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GFB47_RS01505 (GFB47_01510) - 337530..337991 (-) 462 WP_153446009.1 pilin -
  GFB47_RS01510 (GFB47_01515) - 338740..339549 (-) 810 WP_153446011.1 DUF547 domain-containing protein -
  GFB47_RS01515 (GFB47_01520) pdhR 339964..340731 (+) 768 WP_153446013.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 61885.96 Da        Isoelectric Point: 5.5501

>NTDB_id=396067 GFB47_RS01500 WP_153446007.1 335828..337516(-) (pilB) [Vibrio algicola strain SM1977]
MNINLASILRQADFLTHEQEQVVVSSVLNDKKSVPVVCVDAGFIDSQALLQQLCQLFALPQAHLDHYSYAEVCEQLGLRD
LITSHQVLPLQIDSQSLLLAVSDPTNTGVEDDFRFATGKQVEFILADCKELEGAIRRLYGRSAGSDSGQGKDITQDELAN
MVEVGSDEIQETEDLSQDEAPVSRFINQILLDAVRKGASDIHFEPYEELYRVRLRCDGILVETNRPAQHLGRRLAARIKI
LSKLDIAERRLPQDGRIKIRLNADTAIDMRVSTLPTLWGEKIVLRLLDSSAANLNIDKLGYSAPQKKLYLEALQQPQGMI
LMTGPTGSGKTVSLYTGLRILNTPDKNISTAEDPVEINLTGVNQVQVKPKIGFTFAAALRAFLRQDPDVVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSSAETIVRLSNMGIEAFNLASSLTLIIAQRLARRLCSHCKKPDPFPDVLRQKLNIAPDV
VIYKAFSQGCPECTGGYSGRVGIYEVMPFSTEIATAVIKKASANDIEMIAVKQGMSTLRQSGIDNLKQGVTSFAELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=396067 GFB47_RS01500 WP_153446007.1 335828..337516(-) (pilB) [Vibrio algicola strain SM1977]
ATGAACATCAACCTCGCCTCCATTTTACGTCAAGCCGACTTCCTGACTCACGAGCAGGAACAGGTGGTGGTGAGCAGCGT
TCTTAACGATAAAAAAAGCGTCCCCGTGGTGTGCGTGGACGCTGGTTTTATAGATAGCCAAGCGTTATTGCAACAACTGT
GTCAATTATTTGCCCTACCCCAAGCTCACCTTGACCATTACAGCTACGCCGAAGTGTGTGAACAACTCGGATTACGCGAT
TTAATCACTAGCCACCAAGTGCTGCCGCTACAGATTGATTCACAAAGCTTATTACTGGCGGTATCCGACCCCACCAATAC
TGGGGTGGAAGACGATTTTCGCTTTGCTACCGGTAAACAAGTCGAATTTATTCTGGCCGATTGTAAAGAGCTGGAAGGCG
CGATCCGCCGCTTGTATGGCCGAAGCGCAGGCAGCGACAGTGGCCAAGGCAAAGACATCACCCAAGACGAACTGGCTAAC
ATGGTGGAAGTTGGCAGTGATGAAATTCAAGAAACTGAAGATCTCAGTCAAGATGAAGCGCCGGTCAGTCGTTTTATCAA
CCAAATTTTGCTCGATGCGGTGCGCAAAGGGGCGTCAGATATTCACTTTGAACCGTATGAAGAATTGTATCGGGTGCGCC
TGCGTTGTGATGGTATTTTGGTTGAAACCAACCGCCCTGCGCAGCATTTAGGACGCCGCCTTGCCGCGCGAATTAAAATA
TTATCTAAGCTCGATATTGCCGAACGCCGCTTGCCGCAAGATGGGCGGATCAAAATTCGCTTAAACGCCGATACCGCGAT
TGATATGCGAGTTTCAACCTTGCCGACCTTATGGGGCGAGAAAATCGTATTGCGTCTGCTCGACAGTAGCGCTGCCAATT
TGAATATCGACAAACTTGGCTACAGTGCCCCACAAAAAAAATTATATTTAGAGGCGCTACAGCAACCGCAAGGCATGATC
TTAATGACCGGCCCAACCGGTAGCGGTAAAACCGTTTCTTTGTACACCGGATTGCGGATTTTAAACACCCCAGATAAAAA
TATATCGACCGCCGAAGATCCGGTCGAGATCAACTTAACCGGAGTCAACCAAGTGCAAGTGAAACCGAAAATTGGTTTTA
CCTTTGCTGCTGCTCTGCGCGCTTTTTTACGTCAAGATCCCGACGTGGTAATGGTGGGCGAAATTCGCGATCTCGAAACC
GCTGAAATTGCAGTAAAAGCGGCGCAAACTGGTCACTTGGTATTATCAACTCTGCATACCAATTCATCCGCCGAAACTAT
TGTGCGTTTGTCGAATATGGGGATTGAGGCGTTTAACCTTGCTTCATCGTTAACTCTTATTATCGCTCAGCGCTTAGCCC
GTCGTTTATGCTCTCACTGTAAAAAGCCCGATCCATTCCCCGATGTATTGCGCCAAAAATTAAATATCGCGCCGGATGTG
GTGATCTACAAAGCCTTTAGCCAAGGCTGCCCAGAATGTACCGGTGGCTATTCTGGCCGAGTAGGGATTTATGAAGTAAT
GCCATTTAGCACCGAAATTGCCACAGCGGTGATCAAAAAAGCCTCTGCCAACGATATTGAAATGATCGCGGTAAAACAAG
GAATGTCGACCCTGCGTCAATCCGGCATTGATAATTTAAAACAAGGTGTGACCAGCTTCGCCGAACTGCAACGCGTGTTG
TATTTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-143)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5Q0TG00

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

69.217

100

0.692
  pilB Vibrio parahaemolyticus RIMD 2210633

68.505

100

0.685
  pilB Vibrio campbellii strain DS40M4

67.26

100

0.673
  pilB Acinetobacter baumannii D1279779

51.149

92.883

0.475
  pilB Legionella pneumophila strain ERS1305867

49.808

92.527

0.461
  pilB Acinetobacter baylyi ADP1

52.174

85.943

0.448
  pilF Neisseria gonorrhoeae MS11

44.504

100

0.447
  pilF Thermus thermophilus HB27

39.106

95.552

0.374

Multiple sequence alignment