Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   GFB47_RS01490 Genome accession   NZ_CP045699
Coordinates   333595..334506 (-) Length   303 a.a.
NCBI ID   WP_153446003.1    Uniprot ID   A0A5Q0TH49
Organism   Vibrio algicola strain SM1977     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 328595..339506
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GFB47_RS01465 (GFB47_01470) - 329901..330038 (-) 138 WP_153445995.1 hypothetical protein -
  GFB47_RS01470 (GFB47_01475) - 330426..331406 (+) 981 WP_153445997.1 MDR family oxidoreductase -
  GFB47_RS01475 (GFB47_01480) yacG 331879..332082 (-) 204 WP_153445999.1 DNA gyrase inhibitor YacG -
  GFB47_RS01480 (GFB47_01485) zapD 332136..332867 (-) 732 WP_407701681.1 cell division protein ZapD -
  GFB47_RS01485 (GFB47_01490) coaE 332979..333590 (-) 612 WP_153446001.1 dephospho-CoA kinase -
  GFB47_RS01490 (GFB47_01495) pilD 333595..334506 (-) 912 WP_153446003.1 A24 family peptidase Machinery gene
  GFB47_RS01495 (GFB47_01500) pilC 334545..335777 (-) 1233 WP_153446005.1 type II secretion system F family protein Machinery gene
  GFB47_RS01500 (GFB47_01505) pilB 335828..337516 (-) 1689 WP_153446007.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GFB47_RS01505 (GFB47_01510) - 337530..337991 (-) 462 WP_153446009.1 pilin -

Sequence

Protein


Download         Length: 303 a.a.        Molecular weight: 34129.66 Da        Isoelectric Point: 7.1057

>NTDB_id=396065 GFB47_RS01490 WP_153446003.1 333595..334506(-) (pilD) [Vibrio algicola strain SM1977]
MIIFDYYPFLFPILAGVFGLLIGSFLNVVIYRLPVIMEREWKQECFDYLSELKIGKKFKNDPEITAHLDELAQPFNLSIP
RSRCPKCGTQIRAVDNIPVISWLFLRGKCRQCANPISPRYPAIELLTALLAVVVTCYFPADWYGLALLFFTFTLIAATFI
DLDTMLLPDQLTLPLLWAGLALSAFGISPISLQDAVIGAMAGYLCLWSVFWAFKLLTGKEGMGYGDFKLLAALGAWLGWQ
QLPLLILLSSVVGLIFGIIQLRQQKKGIDMTFPFGPYLAIAGWFCLIWGHDIAHWYFTYMLGA

Nucleotide


Download         Length: 912 bp        

>NTDB_id=396065 GFB47_RS01490 WP_153446003.1 333595..334506(-) (pilD) [Vibrio algicola strain SM1977]
ATGATAATTTTTGACTATTACCCTTTTCTATTCCCTATTTTAGCAGGTGTGTTTGGCTTGCTGATTGGCAGTTTTTTAAA
TGTGGTGATCTATCGCCTACCCGTAATCATGGAACGCGAATGGAAACAAGAGTGCTTCGACTACCTTAGTGAACTCAAGA
TTGGTAAAAAGTTTAAAAATGATCCCGAAATAACCGCGCATTTAGATGAGTTAGCACAACCGTTTAATTTAAGCATTCCA
CGTTCACGCTGCCCTAAGTGTGGGACTCAAATTCGCGCAGTCGATAATATTCCCGTGATCAGCTGGTTATTTTTACGTGG
TAAATGCCGTCAATGTGCCAACCCAATTAGCCCTCGCTACCCTGCTATCGAATTATTAACTGCTCTGCTTGCGGTAGTGG
TTACCTGTTATTTCCCTGCCGATTGGTATGGCTTAGCGTTATTGTTTTTCACCTTTACCTTAATAGCGGCCACCTTTATC
GATCTCGATACCATGTTGTTGCCTGACCAATTAACCCTGCCGTTATTATGGGCGGGATTGGCATTATCGGCGTTTGGTAT
TAGCCCGATTTCATTACAAGATGCGGTGATTGGCGCAATGGCGGGTTACTTATGCTTATGGTCGGTATTTTGGGCATTCA
AATTACTCACTGGTAAAGAAGGCATGGGGTATGGCGATTTTAAATTGCTGGCCGCTCTTGGTGCATGGCTTGGATGGCAA
CAATTACCGTTATTAATATTACTCTCTTCGGTGGTGGGACTCATCTTTGGCATTATTCAATTACGCCAGCAGAAAAAAGG
CATCGATATGACCTTTCCATTTGGCCCCTACCTTGCCATCGCCGGGTGGTTTTGTTTGATTTGGGGGCACGATATCGCCC
ATTGGTACTTCACTTATATGCTAGGTGCGTAA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(17-137)

Type IV leader peptidase family (Peptidase_A24)

(149-258)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5Q0TH49

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio campbellii strain DS40M4

65.677

100

0.657
  pilD Vibrio cholerae strain A1552

65.894

99.67

0.657
  pilD Acinetobacter nosocomialis M2

50.526

94.059

0.475
  pilD Acinetobacter baumannii D1279779

50.526

94.059

0.475
  pilD Neisseria gonorrhoeae MS11

46.78

97.36

0.455

Multiple sequence alignment