Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   GAY96_RS11710 Genome accession   NZ_CP045550
Coordinates   2479432..2481555 (-) Length   707 a.a.
NCBI ID   WP_265573632.1    Uniprot ID   -
Organism   Venatoribacter cucullus strain ASxS5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2474432..2486555
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GAY96_RS11695 (GAY96_11630) - 2476289..2477779 (-) 1491 WP_265573630.1 AAA family ATPase -
  GAY96_RS11700 (GAY96_11635) aroB 2477807..2478907 (-) 1101 WP_265573631.1 3-dehydroquinate synthase -
  GAY96_RS11705 (GAY96_11640) aroK 2478913..2479428 (-) 516 WP_228345187.1 shikimate kinase AroK -
  GAY96_RS11710 (GAY96_11645) pilQ 2479432..2481555 (-) 2124 WP_265573632.1 type IV pilus secretin PilQ family protein Machinery gene
  GAY96_RS11715 (GAY96_11650) - 2481560..2482093 (-) 534 WP_228345189.1 pilus assembly protein PilP -
  GAY96_RS11720 (GAY96_11655) - 2482090..2482755 (-) 666 WP_228345190.1 type 4a pilus biogenesis protein PilO -
  GAY96_RS11725 (GAY96_11660) - 2482759..2483307 (-) 549 WP_228345191.1 PilN domain-containing protein -
  GAY96_RS11730 (GAY96_11665) pilM 2483294..2484367 (-) 1074 WP_265574835.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 707 a.a.        Molecular weight: 76346.87 Da        Isoelectric Point: 4.7731

>NTDB_id=394981 GAY96_RS11710 WP_265573632.1 2479432..2481555(-) (pilQ) [Venatoribacter cucullus strain ASxS5]
MNMVKRVRVAIKSVPVLLLTMLAANASALTLTDMTFSALPGDVTEVRLDFDGTPPAISGYTIEQPARIAIDLPATENGLK
TRRHEIGSGNARSAVVVATKDRSRIVFNLTRAVGYTTRVEGNAVLLRIGQTESDKVFAAQPAAPAGSAQAPVAAASSARD
MRVVNVDFRRGQQGEGQVQIMLSDDRAAANIRREGTKIIAELDNVRLPDNLSRRLDVTDFSTPVRFVDARAEGRGTRIVI
EPNGDEFEYLAYQTDRLLSINVSVLPPEQKEDRKKQMFPFTGEKLSLNFQNIEVRAVLQLIADFTGMNLVASDTVQGSIT
LRLQNVPWDQALDLVLKTKGLGKRQMGSVLLIAPAEEIAAREKIELEAVRQVDELAPLVTEYMQLKYAKASVLVNLLTSE
KGLLSERGSAVVDERTNTLLMKDTAKNLEKVREALMMLDVPVRQVLIEARIVVASTSVGEEMGVKWGGAGYKNNGSNWTT
IGGSQQTLTEGNQILFDRASGSAAGSSAIDIGAANMVDFGVTNASASTFAIGYQTADYLLDLELAAIETDGRAEIVSQPR
VITADGQTASIESGTEIPYQEASSSGATSVSFKSAVLKLEVTPQITPDDRIIMDLVINQDSVGELTAAGPSINTNSVQTQ
VLVDNGETVVLGGIFRSEEIVSISKTPFFGDLPLIGALFRYTNRADDKSELLVFITPRLVKDSLTNR

Nucleotide


Download         Length: 2124 bp        

>NTDB_id=394981 GAY96_RS11710 WP_265573632.1 2479432..2481555(-) (pilQ) [Venatoribacter cucullus strain ASxS5]
ATGAACATGGTTAAGCGCGTACGTGTGGCAATTAAGTCAGTGCCGGTCCTGCTGCTGACGATGCTGGCGGCTAATGCCTC
GGCTCTGACCCTGACGGATATGACCTTCTCGGCGTTGCCCGGTGATGTCACGGAGGTGCGACTCGATTTTGACGGCACTC
CGCCCGCCATCAGTGGCTACACCATTGAACAGCCGGCCCGGATCGCCATTGACTTACCTGCTACTGAAAATGGTCTGAAA
ACCCGTCGCCATGAAATCGGCTCCGGCAATGCCCGCAGCGCGGTGGTGGTGGCAACCAAAGATCGTTCCCGCATTGTGTT
TAACCTGACCCGGGCGGTGGGTTACACCACCCGCGTTGAAGGTAATGCCGTGCTGCTGCGTATTGGCCAGACCGAAAGCG
ATAAAGTGTTTGCCGCCCAACCTGCTGCCCCGGCTGGCAGCGCTCAGGCCCCGGTTGCTGCCGCATCTTCAGCCCGTGAT
ATGCGTGTCGTGAATGTCGATTTCCGCCGTGGACAGCAAGGTGAAGGCCAGGTGCAGATTATGCTCAGCGATGATCGTGC
CGCTGCCAATATCCGTCGTGAAGGCACCAAGATTATTGCCGAGCTGGATAACGTCCGCCTGCCGGATAACCTCAGCCGTC
GTCTGGATGTTACCGATTTTTCCACCCCGGTCCGCTTTGTCGATGCCCGCGCTGAAGGTCGTGGTACCCGCATTGTGATT
GAGCCGAATGGTGATGAATTTGAATATCTGGCCTACCAGACCGATCGTCTGCTGTCGATCAACGTCTCGGTGTTGCCGCC
GGAACAAAAAGAAGACCGCAAAAAGCAGATGTTCCCGTTCACCGGCGAAAAACTGTCGCTGAACTTCCAGAACATCGAAG
TGCGTGCGGTACTGCAGCTGATTGCTGATTTCACCGGCATGAACCTGGTGGCGTCCGATACCGTGCAGGGCAGCATTACC
TTGCGTCTGCAGAATGTACCCTGGGATCAGGCGCTGGATCTGGTACTGAAAACCAAAGGTCTGGGCAAACGCCAGATGGG
CTCGGTACTGCTGATTGCGCCGGCGGAAGAAATTGCCGCGCGGGAAAAAATCGAGCTGGAAGCTGTGCGTCAGGTCGACG
AACTGGCTCCGCTGGTCACTGAATACATGCAGCTGAAATACGCCAAAGCCAGTGTGCTGGTGAATCTGCTGACCTCAGAA
AAAGGCCTGTTGTCTGAGCGCGGCTCCGCGGTCGTTGATGAGCGTACCAACACGCTGCTGATGAAAGACACCGCCAAAAA
CCTGGAGAAAGTCCGCGAAGCACTGATGATGCTGGACGTACCGGTGCGCCAGGTACTGATCGAAGCCCGTATCGTGGTCG
CCAGCACCAGTGTGGGTGAAGAGATGGGCGTTAAATGGGGCGGTGCCGGTTATAAAAACAACGGCAGCAACTGGACCACT
ATCGGCGGCAGCCAGCAGACGCTGACCGAAGGCAATCAGATTCTGTTCGATCGTGCATCAGGAAGTGCTGCTGGTTCTTC
CGCCATTGATATTGGTGCGGCCAATATGGTTGATTTCGGCGTAACCAATGCCTCTGCGTCGACTTTCGCCATTGGTTATC
AGACCGCTGATTACCTGTTGGATCTGGAATTAGCCGCCATTGAAACCGACGGCCGTGCTGAAATTGTGTCGCAGCCGCGG
GTGATTACCGCCGATGGCCAGACCGCTTCGATTGAATCCGGTACGGAAATTCCGTATCAGGAAGCCAGTTCCAGCGGTGC
CACCAGCGTGTCGTTCAAATCGGCCGTGCTGAAGCTGGAAGTAACCCCGCAAATCACCCCGGATGACCGCATCATCATGG
ATCTGGTCATCAACCAGGATTCCGTCGGTGAGCTGACCGCTGCCGGCCCCAGCATCAATACCAACTCGGTACAGACGCAG
GTACTGGTCGATAATGGCGAAACCGTGGTGCTGGGTGGTATTTTCCGTTCGGAAGAAATTGTTTCCATCAGCAAGACGCC
GTTCTTCGGTGATCTGCCACTGATCGGTGCATTGTTCCGTTACACCAACCGTGCTGATGACAAGAGCGAACTGCTGGTGT
TTATCACTCCACGACTGGTGAAGGACTCGCTCACGAACCGCTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(44-123)

Bacterial type II/III secretion system short domain (Secretin_N)

(382-444)

Bacterial type II and III secretion system protein (Secretin)

(546-701)

Secretin and TonB N terminus short domain (STN)

(308-354)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

48.237

100

0.484
  pilQ Acinetobacter baumannii D1279779

41.064

100

0.426
  pilQ Acinetobacter baumannii strain A118

40.928

100

0.424
  comQ Acinetobacter baylyi ADP1

39.722

100

0.405
  pilQ Legionella pneumophila strain Lp02

38.953

97.313

0.379
  pilQ Legionella pneumophila strain ERS1305867

38.808

97.313

0.378

Multiple sequence alignment