Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   GAY96_RS11730 Genome accession   NZ_CP045550
Coordinates   2483294..2484367 (-) Length   357 a.a.
NCBI ID   WP_265574835.1    Uniprot ID   -
Organism   Venatoribacter cucullus strain ASxS5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2478294..2489367
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GAY96_RS11705 (GAY96_11640) aroK 2478913..2479428 (-) 516 WP_228345187.1 shikimate kinase AroK -
  GAY96_RS11710 (GAY96_11645) pilQ 2479432..2481555 (-) 2124 WP_265573632.1 type IV pilus secretin PilQ family protein Machinery gene
  GAY96_RS11715 (GAY96_11650) - 2481560..2482093 (-) 534 WP_228345189.1 pilus assembly protein PilP -
  GAY96_RS11720 (GAY96_11655) - 2482090..2482755 (-) 666 WP_228345190.1 type 4a pilus biogenesis protein PilO -
  GAY96_RS11725 (GAY96_11660) - 2482759..2483307 (-) 549 WP_228345191.1 PilN domain-containing protein -
  GAY96_RS11730 (GAY96_11665) pilM 2483294..2484367 (-) 1074 WP_265574835.1 pilus assembly protein PilM Machinery gene
  GAY96_RS11735 (GAY96_11670) - 2484533..2487013 (+) 2481 WP_265573633.1 penicillin-binding protein 1A -
  GAY96_RS11740 (GAY96_11680) - 2487289..2488707 (-) 1419 WP_265573634.1 FAD-binding oxidoreductase -

Sequence

Protein


Download         Length: 357 a.a.        Molecular weight: 38933.88 Da        Isoelectric Point: 4.3653

>NTDB_id=394982 GAY96_RS11730 WP_265574835.1 2483294..2484367(-) (pilM) [Venatoribacter cucullus strain ASxS5]
MANLFKKKSKALLGVDISSTSVKILELSHNNGRYQVEAYACESLPPNAIVEQSINNDEAVGEAIKRALTRSRAGARRAAI
AVAGSAVITKTVQMNGKLSDDEMDFQIRAEADQYIPYPLEEVALDWEVQGPSAGGNDMVDVLLAACRSETVERRKDAVEY
ANLEAGVVDVEAFCTERAFSLLQGQIDGDDIDTVAIIDVGATMTTLSILHEGKSIYTREQLFGGRQLTEDIMRRYGLSEE
EASRAKLEGGLPDDYETEVLEPFRRAVVQQVSRSLQFFYSSSQFNDVDYIILAGGTASIPHLADQVQDAMGIATIVANPF
VNMTLSQKVNPNLLNSDAPSLMIACGLAMRSFDNGKY

Nucleotide


Download         Length: 1074 bp        

>NTDB_id=394982 GAY96_RS11730 WP_265574835.1 2483294..2484367(-) (pilM) [Venatoribacter cucullus strain ASxS5]
CTGGCCAACCTGTTTAAGAAGAAGAGCAAGGCCTTGCTGGGGGTCGATATCAGTTCGACATCGGTTAAGATTCTTGAACT
GAGTCACAATAATGGAAGATATCAGGTTGAAGCCTATGCGTGCGAATCTCTGCCGCCTAATGCCATTGTTGAACAAAGCA
TTAATAATGACGAAGCCGTTGGTGAAGCGATCAAGCGTGCCTTGACCCGTTCCCGTGCCGGTGCCCGTCGTGCTGCCATT
GCGGTTGCCGGCTCTGCCGTTATTACCAAAACGGTGCAGATGAATGGCAAGCTCAGCGATGACGAGATGGATTTCCAGAT
CCGCGCTGAAGCGGATCAGTACATTCCTTATCCGCTGGAAGAAGTGGCGCTGGACTGGGAAGTACAGGGGCCGTCTGCCG
GTGGTAACGATATGGTAGATGTGCTGCTGGCGGCCTGCCGTTCTGAGACGGTTGAGCGGCGTAAAGATGCGGTCGAATAC
GCCAATCTTGAAGCCGGTGTTGTTGATGTCGAAGCGTTCTGTACGGAACGCGCCTTTTCTTTGCTGCAAGGCCAGATAGA
TGGCGACGATATCGATACCGTCGCCATTATTGATGTCGGCGCGACCATGACCACTCTCAGCATTCTGCATGAAGGCAAAT
CTATTTATACCCGCGAGCAGTTATTCGGCGGTCGTCAGCTGACCGAAGATATTATGCGTCGCTATGGTCTCAGCGAAGAG
GAGGCCAGCCGCGCCAAGCTCGAAGGGGGCTTGCCGGACGATTATGAAACGGAAGTGCTGGAGCCTTTCCGTCGTGCGGT
GGTGCAGCAGGTCAGTCGCTCGCTGCAGTTCTTTTATTCCTCCAGTCAGTTCAATGATGTGGATTACATCATTCTGGCGG
GCGGTACCGCTTCCATTCCCCATCTGGCCGATCAGGTTCAGGATGCCATGGGTATTGCTACCATTGTGGCCAATCCTTTC
GTCAATATGACGTTATCGCAGAAGGTAAACCCGAATCTGCTCAACAGTGACGCACCATCACTGATGATTGCCTGTGGTCT
GGCTATGAGGAGTTTTGATAATGGCAAATATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-352)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

54.108

98.88

0.535
  comM Acinetobacter nosocomialis M2

54.108

98.88

0.535
  comM Acinetobacter baylyi ADP1

49.008

98.88

0.485
  pilM Legionella pneumophila strain ERS1305867

44.633

99.16

0.443

Multiple sequence alignment