Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CWC22_RS17220 Genome accession   NZ_CP045429
Coordinates   4011457..4013133 (+) Length   558 a.a.
NCBI ID   WP_125559025.1    Uniprot ID   A0A5S3URP4
Organism   Pseudoalteromonas rubra strain S4059     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4006457..4018133
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CWC22_RS17195 (CWC22_017120) nadC 4006848..4007687 (+) 840 WP_138539314.1 carboxylating nicotinate-nucleotide diphosphorylase -
  CWC22_RS24600 (CWC22_017125) - 4008085..4008552 (+) 468 WP_138539313.1 pilin -
  CWC22_RS17205 (CWC22_017130) pilC 4008602..4009834 (+) 1233 WP_125559031.1 type II secretion system F family protein Machinery gene
  CWC22_RS17210 (CWC22_017135) pilD 4009857..4010732 (+) 876 WP_125559029.1 prepilin peptidase Machinery gene
  CWC22_RS17215 (CWC22_017140) coaE 4010737..4011348 (+) 612 WP_138539312.1 dephospho-CoA kinase -
  CWC22_RS17220 (CWC22_017145) pilB 4011457..4013133 (+) 1677 WP_125559025.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CWC22_RS17225 (CWC22_017150) yacG 4013205..4013447 (+) 243 WP_010383557.1 DNA gyrase inhibitor YacG -
  CWC22_RS17230 (CWC22_017155) - 4013494..4014003 (-) 510 WP_138539311.1 Spy/CpxP family protein refolding chaperone -
  CWC22_RS17235 (CWC22_017160) - 4014212..4014898 (+) 687 WP_138539334.1 response regulator transcription factor -
  CWC22_RS17240 (CWC22_017165) - 4014900..4016279 (+) 1380 WP_138539310.1 ATP-binding protein -
  CWC22_RS17245 (CWC22_017170) - 4016269..4017249 (+) 981 WP_138539309.1 alpha/beta fold hydrolase -
  CWC22_RS17250 (CWC22_017175) - 4017242..4018066 (-) 825 WP_040644593.1 cation diffusion facilitator family transporter -

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 62265.33 Da        Isoelectric Point: 5.6781

>NTDB_id=394069 CWC22_RS17220 WP_125559025.1 4011457..4013133(+) (pilB) [Pseudoalteromonas rubra strain S4059]
MEHHSPLLRKFITLGKLTAEQVKAKQADAHSTAELICLCSGMSSQEMAEQCLDLFRVPYFDLNGFDITQVPKELVKEKLI
RQHHLLPLVKKDRKLFIATSDPTDYGAFENFEFSTGLQCEVVVVDYKLLDQKIDQLFDDNSSLSLSEDEFKEFASLDVED
EPKQQNTDEEKDDAPIIVYINKILMDAIKKGASDLHFEPYEKKYRVRFRIDGLLHEMASPPHALSSRLSARIKVMSHLDI
AEKRKPQDGRIKLKISERKSIDFRVSTLPTMWGEKIVMRILDSSAAKLGIEALGYEADQKSMYLNALDQPQGMILVTGPT
GSGKTVSLYTGLNILNKPERNISTAEDPVEINLEGINQVQINTKADMTFANALRAFLRQDPDVVMVGEIRDLETAEIAIK
AAQTGHLVMSTLHTNSAAETLTRLLNMGVPAYNVASSVSLIIAQRLARRLCPKCKEPEQLPTEELQRQGFSADQIENMTL
YAPKGCDHCTDGYKGRVGIYEVMKITPEISHAIMEGGNSLEIAELATSKGFANLRQSGLKKAADGITSLAEVNRVTNY

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=394069 CWC22_RS17220 WP_125559025.1 4011457..4013133(+) (pilB) [Pseudoalteromonas rubra strain S4059]
ATGGAACATCACTCGCCGTTACTGAGAAAGTTTATTACCCTTGGCAAACTGACTGCCGAGCAGGTCAAAGCCAAACAAGC
AGACGCCCATTCAACGGCTGAGCTCATCTGCCTGTGTAGTGGCATGAGCAGCCAGGAGATGGCCGAGCAATGTCTGGACT
TATTCAGAGTACCTTATTTCGATCTGAACGGCTTTGACATTACCCAGGTTCCCAAGGAGCTGGTCAAAGAAAAGTTGATC
CGTCAGCACCACTTGCTGCCTCTGGTCAAAAAAGATCGTAAGCTCTTTATTGCGACTTCAGATCCCACAGATTACGGCGC
ATTCGAGAACTTTGAGTTCAGCACCGGCCTGCAGTGTGAAGTGGTGGTTGTAGACTATAAGCTACTCGATCAGAAGATTG
ACCAACTTTTTGACGATAATAGCTCTCTCAGCCTCAGTGAAGACGAGTTTAAAGAATTTGCCAGCCTGGATGTCGAAGAC
GAGCCCAAACAACAGAACACCGACGAAGAAAAAGACGATGCGCCTATCATCGTGTACATCAACAAGATCCTCATGGATGC
AATTAAAAAAGGCGCCTCGGATCTCCATTTCGAACCCTATGAAAAGAAATATCGGGTGCGCTTTCGGATCGACGGCTTAT
TACATGAAATGGCGAGCCCACCGCATGCACTTTCCAGCCGTTTGTCGGCGCGCATCAAAGTCATGTCTCATCTGGATATC
GCCGAAAAGCGAAAACCCCAAGATGGCCGCATCAAACTGAAAATATCAGAGCGCAAAAGCATCGATTTCCGTGTTAGTAC
TCTGCCCACCATGTGGGGCGAAAAAATCGTAATGCGGATCCTCGATTCATCAGCCGCCAAACTGGGCATTGAAGCACTGG
GTTATGAAGCAGATCAGAAAAGTATGTACCTAAATGCACTCGATCAGCCTCAGGGGATGATCCTGGTCACCGGACCGACT
GGCTCAGGTAAAACAGTCTCGCTTTACACCGGCCTGAATATACTCAATAAACCAGAGCGCAACATCAGCACTGCGGAAGA
TCCAGTCGAGATTAACCTGGAAGGTATCAACCAGGTTCAAATCAATACCAAAGCGGACATGACCTTTGCCAATGCACTGC
GGGCATTCCTGCGCCAGGATCCCGATGTGGTCATGGTTGGTGAGATCCGGGATCTGGAAACTGCAGAGATTGCCATCAAG
GCAGCACAAACCGGTCACCTGGTTATGAGTACCTTGCACACCAATTCAGCCGCAGAAACCCTGACCCGCTTACTTAACAT
GGGCGTACCGGCCTATAATGTGGCCAGCTCTGTCAGCCTGATCATTGCCCAGCGCCTTGCTCGTCGATTGTGCCCCAAGT
GCAAAGAGCCAGAACAATTGCCCACAGAGGAATTACAACGTCAGGGCTTTAGCGCAGATCAAATTGAGAATATGACCCTC
TATGCGCCCAAAGGATGTGATCACTGTACCGATGGCTATAAAGGCCGGGTAGGTATTTACGAGGTAATGAAGATCACTCC
GGAAATCTCGCATGCCATCATGGAAGGCGGTAACTCCCTGGAGATTGCTGAACTTGCTACAAGTAAAGGCTTTGCAAATC
TGCGTCAATCCGGATTGAAAAAAGCCGCGGACGGGATCACCTCACTGGCAGAGGTCAACCGGGTAACGAACTACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(182-449)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5S3URP4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.074

96.774

0.572
  pilB Acinetobacter baylyi ADP1

55.319

100

0.559
  pilB Vibrio cholerae strain A1552

53.986

98.925

0.534
  pilB Vibrio parahaemolyticus RIMD 2210633

54.512

97.312

0.53
  pilB Legionella pneumophila strain ERS1305867

56.046

93.369

0.523
  pilB Vibrio campbellii strain DS40M4

53.407

97.312

0.52
  pilF Neisseria gonorrhoeae MS11

47.133

100

0.471
  pilB/pilB1 Synechocystis sp. PCC 6803

39.458

92.652

0.366

Multiple sequence alignment