Detailed information NTG0394068

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: CWC22_RS17210
• Genome accession: NZ_CP045429
• Coordinates: 4009857..4010732 (+)
• Length: 291 a.a.
• NCBI ID: WP_125559029.1
• Uniprot ID: A0A5S3US46
• Organism: Pseudoalteromonas rubra strain S4059
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4004857..4015732

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CWC22_RS17185 (CWC22_017110)	ampE	4005226..4006110 (-)	885	WP_138539316.1	beta-lactamase regulator AmpE	-
CWC22_RS17190 (CWC22_017115)	ampD	4006122..4006658 (-)	537	WP_138539315.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
CWC22_RS17195 (CWC22_017120)	nadC	4006848..4007687 (+)	840	WP_138539314.1	carboxylating nicotinate-nucleotide diphosphorylase	-
CWC22_RS24600 (CWC22_017125)	-	4008085..4008552 (+)	468	WP_138539313.1	pilin	-
CWC22_RS17205 (CWC22_017130)	pilC	4008602..4009834 (+)	1233	WP_125559031.1	type II secretion system F family protein	Machinery gene
CWC22_RS17210 (CWC22_017135)	pilD	4009857..4010732 (+)	876	WP_125559029.1	prepilin peptidase	Machinery gene
CWC22_RS17215 (CWC22_017140)	coaE	4010737..4011348 (+)	612	WP_138539312.1	dephospho-CoA kinase	-
CWC22_RS17220 (CWC22_017145)	pilB	4011457..4013133 (+)	1677	WP_125559025.1	type IV-A pilus assembly ATPase PilB	Machinery gene
CWC22_RS17225 (CWC22_017150)	yacG	4013205..4013447 (+)	243	WP_010383557.1	DNA gyrase inhibitor YacG	-
CWC22_RS17230 (CWC22_017155)	-	4013494..4014003 (-)	510	WP_138539311.1	Spy/CpxP family protein refolding chaperone	-
CWC22_RS17235 (CWC22_017160)	-	4014212..4014898 (+)	687	WP_138539334.1	response regulator transcription factor	-

Sequence

Protein

• Length: 291 a.a.
• Molecular weight: 32301.54 Da
• Isoelectric Point: 7.2752

>NTDB_id=394068 CWC22_RS17210 WP_125559029.1 4009857..4010732(+) (pilD) [Pseudoalteromonas rubra strain S4059]
MQEVWHLMEQQTWFWLLTVALTSVCIGSFLNVVIYRLPIMMQRNWQSECRIILEQAPQQAEPATFNLMAPGSTCPKCNTK
IKLHHNIPLLSWLLLGGKCAYCDTPIPKRYPLVELFTGVASLWLAWHFSATPQALVYILVTWVLIALIFIDIDHMLLPDQ
LTLPLLWFALLAAVAGITIPPSEAIIGAAVGYLSLWSIYWGFKLLTGKEGMGFGDFKLLAVFGALMGWQSLLLIVLLSSV
VGAVIGATQLAVQGKDKASPIPFGPYLAIAGWLTLLYGEQLSRWYLSLLGV

Nucleotide

• Length: 876 bp

>NTDB_id=394068 CWC22_RS17210 WP_125559029.1 4009857..4010732(+) (pilD) [Pseudoalteromonas rubra strain S4059]
ATGCAAGAAGTTTGGCACCTTATGGAGCAGCAAACCTGGTTTTGGTTGCTGACTGTCGCACTGACCAGTGTGTGTATCGG
CAGCTTTTTAAACGTTGTCATTTATCGCTTACCCATTATGATGCAACGCAACTGGCAGAGCGAATGCCGAATAATTCTGG
AGCAGGCGCCACAGCAAGCTGAACCTGCCACTTTTAATTTGATGGCACCCGGGTCTACCTGCCCTAAGTGCAATACTAAG
ATCAAACTACATCATAATATTCCCTTGCTCAGTTGGTTACTGCTCGGTGGCAAATGCGCATACTGTGATACGCCAATTCC
CAAGCGCTATCCGTTGGTCGAGTTATTTACAGGCGTGGCTTCACTATGGTTGGCATGGCATTTCTCAGCCACGCCCCAAG
CGCTGGTATACATACTGGTAACCTGGGTACTAATTGCACTGATCTTCATTGATATTGATCATATGCTGCTGCCCGACCAA
TTAACCCTCCCCTTACTTTGGTTTGCTCTGTTGGCGGCCGTGGCAGGCATCACCATTCCGCCATCTGAAGCCATTATTGG
TGCGGCCGTTGGTTATCTGAGTTTATGGAGCATTTACTGGGGATTCAAGCTATTGACCGGCAAAGAAGGCATGGGATTTG
GTGATTTTAAACTATTAGCCGTATTTGGTGCGCTGATGGGCTGGCAGTCACTGCTACTCATCGTGCTGCTCTCAAGTGTT
GTTGGGGCAGTGATCGGCGCAACACAACTCGCTGTGCAAGGCAAAGATAAAGCCAGCCCTATCCCCTTTGGTCCTTACCT
CGCGATTGCCGGCTGGCTAACGCTATTGTACGGTGAGCAACTCAGCCGCTGGTATCTGTCGTTATTAGGAGTATAG

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (139-246)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (22-128)

3D structure

• AlphaFold DB: A0A5S3US46

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	52.899	94.845	0.502
pilD	Vibrio campbellii strain DS40M4	50	98.969	0.495
pilD	Acinetobacter nosocomialis M2	48.084	98.625	0.474
pilD	Acinetobacter baumannii D1279779	47.735	98.625	0.471
pilD	Neisseria gonorrhoeae MS11	43.173	93.127	0.402