Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   FIU96_RS13050 Genome accession   NZ_CP045367
Coordinates   2817246..2817695 (-) Length   149 a.a.
NCBI ID   WP_323847456.1    Uniprot ID   -
Organism   Marinobacter sp. THAF39     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 2807198..2817115 2817246..2817695 flank 131


Gene organization within MGE regions


Location: 2807198..2817695
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FIU96_RS13015 (FIU96_13125) - 2807198..2808310 (+) 1113 WP_152438641.1 glycosyltransferase -
  FIU96_RS13020 (FIU96_13130) - 2808234..2809490 (-) 1257 WP_152438642.1 lipopolysaccharide biosynthesis protein -
  FIU96_RS13025 (FIU96_13135) asnB 2809755..2811620 (-) 1866 WP_152438643.1 asparagine synthase (glutamine-hydrolyzing) -
  FIU96_RS13030 (FIU96_13145) - 2811927..2813036 (-) 1110 WP_152438644.1 glycosyltransferase -
  FIU96_RS13035 (FIU96_13150) - 2813093..2814268 (-) 1176 WP_152438645.1 glycosyltransferase family 4 protein -
  FIU96_RS13040 (FIU96_13155) - 2814314..2815168 (-) 855 WP_216646150.1 glycosyltransferase family 2 protein -
  FIU96_RS13045 (FIU96_13160) - 2815238..2817142 (-) 1905 WP_152438647.1 tetratricopeptide repeat protein -
  FIU96_RS13050 (FIU96_13165) pilA2 2817246..2817695 (-) 450 WP_323847456.1 pilin Machinery gene

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 15604.66 Da        Isoelectric Point: 4.9812

>NTDB_id=393684 FIU96_RS13050 WP_323847456.1 2817246..2817695(-) (pilA2) [Marinobacter sp. THAF39]
MQKIQMKKGQQGFTLIELMIVVAIIGILAAVAIPAYQDYTIRAKVSEVLTVASAARTSVAEYYISTGSMPATTTAAGINT
DTGQSDYITNIAFATSSTGTQLTYTLGNLGGTADASTFEYLVTGNNNGVQVDCTGGSLEAKYRPANCRP

Nucleotide


Download         Length: 450 bp        

>NTDB_id=393684 FIU96_RS13050 WP_323847456.1 2817246..2817695(-) (pilA2) [Marinobacter sp. THAF39]
ATGCAGAAGATTCAAATGAAAAAAGGTCAGCAGGGTTTCACTCTGATCGAACTGATGATCGTTGTTGCAATCATTGGTAT
TTTGGCGGCTGTTGCTATTCCTGCGTATCAGGACTACACAATCCGTGCCAAGGTTAGTGAGGTTTTGACGGTTGCTAGTG
CTGCCCGCACAAGTGTCGCCGAATACTACATCAGCACTGGTAGCATGCCCGCGACCACAACCGCTGCTGGCATTAACACA
GATACCGGCCAGAGCGATTACATCACTAATATTGCTTTTGCTACTTCTTCTACTGGTACTCAGTTGACCTACACATTGGG
CAATTTGGGTGGTACTGCAGACGCCAGCACTTTTGAATACCTCGTAACTGGTAATAACAATGGCGTTCAAGTTGATTGCA
CAGGCGGTTCCCTCGAGGCTAAATATCGTCCTGCAAACTGTCGCCCGTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(42-148)

Prokaryotic N-terminal methylation motif (N_methyl)

(7-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila strain ERS1305867

51.064

94.631

0.483
  pilA2 Legionella pneumophila str. Paris

51.064

94.631

0.483
  pilA Ralstonia pseudosolanacearum GMI1000

39.759

100

0.443
  comP Acinetobacter baylyi ADP1

42.857

98.658

0.423
  pilE Neisseria gonorrhoeae MS11

37.821

100

0.396
  pilE Neisseria gonorrhoeae strain FA1090

37.419

100

0.389
  pilA/pilAI Pseudomonas stutzeri DSM 10701

38.356

97.987

0.376

Multiple sequence alignment