Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HP434_RS17725 Genome accession   NZ_CP053720
Coordinates   3697822..3699207 (+) Length   461 a.a.
NCBI ID   WP_096176639.1    Uniprot ID   -
Organism   Escherichia coli strain CP131_Sichuan     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3692822..3704207
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HP434_RS17700 (HP434_17710) aroP 3693370..3694743 (+) 1374 WP_000969912.1 aromatic amino acid transporter AroP -
  HP434_RS17705 (HP434_17715) ampE 3694786..3695640 (-) 855 WP_000172014.1 beta-lactamase regulator AmpE -
  HP434_RS17710 (HP434_17720) ampD 3695637..3696188 (-) 552 WP_000923722.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HP434_RS17715 (HP434_17725) nadC 3696276..3697169 (+) 894 WP_001135176.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HP434_RS17720 (HP434_17730) pilA 3697372..3697812 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  HP434_RS17725 (HP434_17735) pilB 3697822..3699207 (+) 1386 WP_096176639.1 type II secretion system protein GspE Machinery gene
  HP434_RS17730 (HP434_17740) hofC 3699197..3700399 (+) 1203 WP_000157266.1 protein transport protein HofC -
  HP434_RS17735 (HP434_17745) guaC 3700434..3701477 (-) 1044 WP_001217338.1 GMP reductase -
  HP434_RS23935 - 3701633..3701677 (-) 45 WP_120795372.1 protein YacM -
  HP434_RS17740 (HP434_17750) coaE 3701702..3702322 (+) 621 WP_001269520.1 dephospho-CoA kinase -
  HP434_RS17745 (HP434_17755) zapD 3702322..3703065 (+) 744 WP_001194722.1 cell division protein ZapD -
  HP434_RS17750 (HP434_17760) yacG 3703075..3703272 (+) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  HP434_RS17755 (HP434_17765) mutT 3703488..3703877 (-) 390 WP_000736009.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50573.25 Da        Isoelectric Point: 6.6612

>NTDB_id=393675 HP434_RS17725 WP_096176639.1 3697822..3699207(+) (pilB) [Escherichia coli strain CP131_Sichuan]
MNIPQLTALCLRYHGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLPCRGGEKVVLRLLQQVGQALDVNTLGMQPLQLADFAHALQQPQGLVLVTGPTGSGKTVTLYSALQKLN
TADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TCETLVRLQQMGGARWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGFYGRTALFE
VLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=393675 HP434_RS17725 WP_096176639.1 3697822..3699207(+) (pilB) [Escherichia coli strain CP131_Sichuan]
ATGAATATTCCACAGCTCACTGCCCTGTGTCTGCGTTATCATGGAGTCTTGCTGGATGCCAGCGAAGAGGTGGTTCATGT
TGCGGTAGTCGATGCACCTTCGCATGAGCTACTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTGTTCAGGAGAAGCATCAGCCC
AAAGCAGAGTTGCTAACTCGAACGTTACAATCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGATGTTTCACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGAAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTACCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGGGTCAGGCACTGGATGTCAACACGCTTGGAATGCAGCCGTTACAACTGGCGGACTTTGCTCATGCCTTGCAACAAC
CACAGGGACTGGTGCTGGTAACTGGCCCTACCGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAAAGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCAGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATCC
GCGTGCCGGACTCACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGATCCTGACGTCATCATGATCGGAGAGATCC
GCGATGGCGAAACAGCAGAGATCGCTATTAAAGCGGCGCAAACTGGTCACCTGGTGTTGTCTACCCTACACACTAATTCC
ACCTGCGAAACGCTGGTACGTTTACAGCAAATGGGGGGCGCCCGCTGGATGCTATCATCGGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGCAAACTTTGCCCACATTGTCGCCGGCAGCAAGGGGAGCCCATCCACATTCCAGACAATGTATGGC
CATCGCCGCTGCCCCACTGGCAGGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGTACGGCCTTATTTGAA
GTTCTGCCCATAACGCCGGTCATTCGTCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACGCACGCACGACA
GGCGGGTATGCGTACGCTTTTTGAAAACGGCTGCCTGGCCGTAGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TACTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(88-353)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

42.236

100

0.443
  pilB Legionella pneumophila strain ERS1305867

50

83.297

0.416
  pilB Glaesserella parasuis strain SC1401

41.253

100

0.414
  pilB Acinetobacter baylyi ADP1

39.744

100

0.403
  pilB Vibrio cholerae strain A1552

46.41

84.599

0.393
  pilB Vibrio parahaemolyticus RIMD 2210633

45.897

84.599

0.388
  pilB Haemophilus influenzae 86-028NP

45.22

83.948

0.38
  pilB Haemophilus influenzae Rd KW20

44.444

83.948

0.373
  pilB Acinetobacter baumannii D1279779

43.401

85.466

0.371
  pilF Neisseria gonorrhoeae MS11

44.156

83.514

0.369