Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HP434_RS17720 Genome accession   NZ_CP053720
Coordinates   3697372..3697812 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain CP131_Sichuan     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3692372..3702812
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HP434_RS17700 (HP434_17710) aroP 3693370..3694743 (+) 1374 WP_000969912.1 aromatic amino acid transporter AroP -
  HP434_RS17705 (HP434_17715) ampE 3694786..3695640 (-) 855 WP_000172014.1 beta-lactamase regulator AmpE -
  HP434_RS17710 (HP434_17720) ampD 3695637..3696188 (-) 552 WP_000923722.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HP434_RS17715 (HP434_17725) nadC 3696276..3697169 (+) 894 WP_001135176.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HP434_RS17720 (HP434_17730) pilA 3697372..3697812 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  HP434_RS17725 (HP434_17735) pilB 3697822..3699207 (+) 1386 WP_096176639.1 type II secretion system protein GspE Machinery gene
  HP434_RS17730 (HP434_17740) hofC 3699197..3700399 (+) 1203 WP_000157266.1 protein transport protein HofC -
  HP434_RS17735 (HP434_17745) guaC 3700434..3701477 (-) 1044 WP_001217338.1 GMP reductase -
  HP434_RS23935 - 3701633..3701677 (-) 45 WP_120795372.1 protein YacM -
  HP434_RS17740 (HP434_17750) coaE 3701702..3702322 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=393674 HP434_RS17720 WP_000360904.1 3697372..3697812(+) (pilA) [Escherichia coli strain CP131_Sichuan]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=393674 HP434_RS17720 WP_000360904.1 3697372..3697812(+) (pilA) [Escherichia coli strain CP131_Sichuan]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363