Detailed information NTG0393528

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: FIV02_RS04025
• Genome accession: NZ_CP045349
• Coordinates: 878451..880154 (-)
• Length: 567 a.a.
• NCBI ID: WP_152487866.1
• Uniprot ID: -
• Organism: Pseudomonas sp. THAF187a
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	880893..893755	878451..880154	flank	739

Gene organization within MGE regions

Location: 878451..893755

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FIV02_RS04025 (FIV02_03985)	pilB	878451..880154 (-)	1704	WP_152487866.1	type IV-A pilus assembly ATPase PilB	Machinery gene
FIV02_RS25085 (FIV02_03990)	pilA	880376..880789 (+)	414	WP_152487867.1	pilin	Machinery gene
FIV02_RS04035 (FIV02_03995)	pilA	880893..881309 (+)	417	WP_152487868.1	pilin	Machinery gene
FIV02_RS04040 (FIV02_04000)	-	881326..883236 (+)	1911	WP_152487869.1	hypothetical protein	-
FIV02_RS04045 (FIV02_04005)	-	883236..884090 (+)	855	WP_152487870.1	glycosyltransferase family 2 protein	-
FIV02_RS04050 (FIV02_04010)	-	884212..885114 (+)	903	WP_152487871.1	glycosyltransferase	-
FIV02_RS04055 (FIV02_04015)	-	885171..886388 (+)	1218	WP_152487872.1	lipopolysaccharide biosynthesis protein	-
FIV02_RS25165	-	886768..887424 (+)	657	WP_371923266.1	class I SAM-dependent methyltransferase	-
FIV02_RS04065 (FIV02_04025)	-	887684..888421 (+)	738	WP_172979125.1	bifunctional 2-polyprenyl-6-hydroxyphenol methylase/3-demethylubiquinol 3-O-methyltransferase UbiG	-
FIV02_RS04070 (FIV02_04030)	-	888418..889281 (+)	864	WP_152487875.1	glycosyltransferase family 2 protein	-
FIV02_RS04075 (FIV02_04035)	-	889278..890393 (-)	1116	WP_152487876.1	glycosyltransferase family 4 protein	-
FIV02_RS04080 (FIV02_04040)	-	890444..892372 (-)	1929	WP_152487877.1	acyltransferase family protein	-
FIV02_RS04085 (FIV02_04045)	-	892445..893755 (-)	1311	WP_152487878.1	nucleotide sugar dehydrogenase	-

Sequence

Protein

• Length: 567 a.a.
• Molecular weight: 62316.28 Da
• Isoelectric Point: 5.3390

>NTDB_id=393528 FIV02_RS04025 WP_152487866.1 878451..880154(-) (pilB) [Pseudomonas sp. THAF187a]
MNESISLSGLARQLAMAGLMDEKAAQQAQQQAQRNKLPLVTYLVQNKLVKSRALAELAAEQFGVAFFDLNSIDKEGQPRE
LVSEKLVRQHRVLPLWRRGNKLFIGISDPTSHQAVTDVQFSTGLTTEAILVEDDKLGDAIDKFFDSANSGMDDLADVDLD
GLDVEAVDDDKPVGDGGADADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKAYRVRFRTDGMLHEVARPPIQLAPRISAR
LKVMAALDISERRKPQDGRIKMKISKTKAIDFRVNTLPTLWGEKIVMRILDPTSAQMGIDALGYEEEQKELYLSALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTPDVNISTAEDPVEINLEGINQVNVNPRQGMDFTQALRAFLRQDPDIIMVGEIRD
LDTASIAVKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPSFNIATSVNLIIAQRLARKLCSSCKKEVDVPRETLLAEGFP
EDKIGSFKIYGPVGCDNCKGGYKGRVGIYEVVKNTANLQRIIMEEGNSIDIATQMRKDGFNDLRTSALLKAMQGVTSLEE
VNRVTKD

Nucleotide

• Length: 1704 bp

>NTDB_id=393528 FIV02_RS04025 WP_152487866.1 878451..880154(-) (pilB) [Pseudomonas sp. THAF187a]
ATGAACGAGAGCATTTCCCTTTCCGGCCTGGCAAGACAACTGGCGATGGCCGGTCTCATGGACGAGAAGGCCGCGCAGCA
GGCCCAGCAGCAGGCGCAGCGCAACAAGTTGCCGCTGGTGACTTACCTCGTGCAGAACAAACTGGTGAAAAGTCGCGCCC
TTGCAGAGCTGGCCGCCGAACAGTTTGGCGTGGCCTTCTTCGACCTCAATTCCATCGACAAGGAAGGGCAACCGCGTGAA
CTCGTCAGCGAGAAGCTGGTACGCCAGCATCGTGTGCTGCCACTGTGGCGACGCGGCAACAAGCTGTTCATCGGCATTTC
TGACCCAACCAGCCACCAGGCTGTTACCGATGTACAGTTCAGCACAGGCCTGACCACCGAAGCGATCCTGGTCGAGGACG
ACAAACTTGGTGACGCTATCGACAAGTTCTTCGACTCAGCCAATAGCGGGATGGACGACCTCGCCGATGTCGATCTTGAT
GGCCTGGACGTCGAAGCAGTAGACGACGACAAACCGGTAGGAGACGGTGGCGCAGACGCCGATGACGCCCCCGTGGTGCG
CTTCGTCAACAAGATGCTGCTGGACGCGATCAAAGGTGGCTCCTCGGACCTGCACTTCGAGCCTTACGAAAAAGCCTATC
GAGTGCGCTTTCGTACCGACGGGATGCTGCATGAAGTCGCGCGCCCTCCCATTCAGCTCGCCCCACGCATCTCCGCCCGC
CTGAAGGTAATGGCTGCACTGGATATTTCCGAGCGTCGCAAGCCTCAGGATGGCCGGATCAAGATGAAGATCTCCAAGAC
CAAGGCCATCGACTTCCGCGTCAATACCTTGCCAACCCTGTGGGGCGAGAAGATCGTGATGCGAATTCTCGACCCAACCA
GTGCGCAGATGGGCATCGATGCACTGGGCTATGAAGAAGAGCAAAAAGAACTTTACCTCAGTGCGCTCAAACAACCCCAG
GGCATGATTCTGGTTACAGGCCCGACCGGCTCCGGCAAGACGGTGTCGCTCTATACCGGCCTGAACATACTCAACACGCC
TGACGTGAACATCTCCACCGCCGAAGACCCCGTGGAGATCAACCTGGAGGGCATCAATCAGGTCAACGTCAATCCACGAC
AGGGCATGGATTTTACCCAGGCCCTGCGCGCCTTCCTGCGTCAGGACCCGGACATCATCATGGTGGGTGAGATTCGCGAC
TTGGACACAGCGTCCATCGCGGTCAAAGCCGCCCAGACCGGACACATGGTGATGTCTACCCTGCACACCAACAGCGCGGC
GGAAACCCTGACCCGCCTGCGTAACATGGGCGTGCCGTCGTTCAACATCGCCACCTCGGTCAACCTGATCATCGCGCAGC
GCCTGGCACGAAAACTGTGCAGCAGTTGCAAGAAAGAGGTGGACGTGCCTCGCGAGACGCTTCTGGCAGAGGGTTTCCCC
GAGGACAAGATCGGCAGCTTCAAGATCTATGGCCCGGTTGGCTGCGACAACTGCAAGGGCGGCTACAAGGGTCGTGTCGG
TATTTATGAAGTGGTTAAAAACACGGCCAACCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATCGATATCGCCACAC
AGATGCGTAAAGACGGCTTTAATGATCTACGCACCTCCGCTTTGCTCAAGGCCATGCAAGGGGTTACCAGCCTTGAGGAA
GTCAACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (191-458)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (43-147)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baumannii D1279779	57.391	100	0.582
pilB	Acinetobacter baylyi ADP1	57.194	99.295	0.568
pilB	Legionella pneumophila strain ERS1305867	54.13	100	0.543
pilF	Neisseria gonorrhoeae MS11	49.027	99.647	0.489
pilB	Vibrio parahaemolyticus RIMD 2210633	47.865	99.118	0.474
pilB	Vibrio campbellii strain DS40M4	47.163	99.471	0.469
pilB	Vibrio cholerae strain A1552	50.191	92.416	0.464
pilB/pilB1	Synechocystis sp. PCC 6803	34.74	100	0.377
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.13	93.298	0.365
pilF	Thermus thermophilus HB27	36.918	98.413	0.363