Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   GCU53_RS09335 Genome accession   NZ_CP045302
Coordinates   1993225..1995339 (-) Length   704 a.a.
NCBI ID   WP_167520048.1    Uniprot ID   -
Organism   Azotobacter salinestris strain KACC 13899     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1988225..2000339
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GCU53_RS09320 - 1989899..1991539 (-) 1641 WP_152387366.1 AAA family ATPase -
  GCU53_RS09325 aroB 1991547..1992650 (-) 1104 WP_152387367.1 3-dehydroquinate synthase -
  GCU53_RS26010 aroK 1992702..1993220 (-) 519 WP_152387368.1 shikimate kinase AroK -
  GCU53_RS09335 pilQ 1993225..1995339 (-) 2115 WP_167520048.1 type IV pilus secretin PilQ Machinery gene
  GCU53_RS09340 pilP 1995393..1995917 (-) 525 WP_152387370.1 type 4a pilus biogenesis lipoprotein PilP -
  GCU53_RS09345 pilO 1995914..1996537 (-) 624 WP_152387371.1 type 4a pilus biogenesis protein PilO -
  GCU53_RS09350 - 1996534..1997190 (-) 657 WP_152387372.1 PilN domain-containing protein -
  GCU53_RS09355 comM 1997191..1998255 (-) 1065 WP_152387373.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 704 a.a.        Molecular weight: 75920.22 Da        Isoelectric Point: 4.6630

>NTDB_id=393273 GCU53_RS09335 WP_167520048.1 1993225..1995339(-) (pilQ) [Azotobacter salinestris strain KACC 13899]
MNSTLSRLGISILAALLSPELLAADLKALDVAALPGDRVELKLSFDEPVPAPRGYTIEQPARIALDLPGVANKLGTKYRE
LGVGNARSVTLVEANERTRMIVNLAALSPYNTRAEGNNLYVVIGESATAPQQARTAPPPVAAASAPLAKPYVPAGKAVQG
IDFQRGEQGEGNVIIDLSSPGMNPDIQEQGGKIRLDFSGAQLPESLRARLDVKDFATPVQFVSASSQGGKASILIEPTGM
YDYLVYQADNRLTVSVKAKAQESNVRPGADRFAYTGEKLSLNFQDIDIRSVLQLIADFTNLNLVASDTVQGNITLRLQNV
PWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELEAQQQIAELAPLRRELVQVNYAKAADIAKLFQSVTSGEEV
GEQRGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSFVNDQWSLYGKDGA
QSIDDGQVFLPGTDKVGNFTLEDGVAPVPFVDMGVANSTSGLGIGFVSDHLILDLQLSAMEKTGNGEVISQPKVLTSDKE
TAKILTGEEIPYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIMEVKVTKDQADFSKALNGVPPINKNEVNAKVLIGD
GETIVIGGVFSNTQAKSVDKVPFLGDLPFIGRLFRRDVVADSKSELLVFLTPRILNNQAIAVSR

Nucleotide


Download         Length: 2115 bp        

>NTDB_id=393273 GCU53_RS09335 WP_167520048.1 1993225..1995339(-) (pilQ) [Azotobacter salinestris strain KACC 13899]
ATGAACAGCACCCTTTCGCGTCTCGGTATTTCGATCCTGGCTGCCCTGCTGTCGCCGGAATTGTTGGCGGCCGATCTCAA
GGCCCTGGACGTGGCCGCCCTGCCTGGCGATCGGGTCGAACTCAAGTTGTCATTCGACGAGCCCGTGCCGGCTCCGCGCG
GCTACACCATCGAGCAGCCGGCCCGTATTGCCCTGGATCTGCCCGGTGTGGCCAACAAACTGGGGACCAAGTACCGTGAG
CTGGGTGTCGGCAACGCCCGCAGTGTCACCTTGGTCGAGGCGAACGAACGCACCCGGATGATCGTCAACCTGGCTGCACT
GTCGCCCTACAACACGCGGGCCGAGGGCAACAACCTCTATGTGGTGATCGGCGAATCGGCCACCGCGCCCCAGCAGGCCC
GCACTGCGCCGCCACCGGTCGCGGCGGCTTCGGCTCCGCTGGCCAAGCCCTACGTGCCGGCAGGCAAGGCGGTTCAGGGC
ATCGACTTCCAGCGGGGCGAGCAGGGCGAGGGCAACGTCATCATCGATCTGTCGAGCCCAGGCATGAATCCCGATATCCA
GGAGCAGGGCGGCAAGATCCGCCTGGATTTTTCGGGGGCGCAGCTGCCGGAGTCCCTGCGGGCACGCCTGGACGTCAAGG
ATTTCGCCACACCCGTGCAGTTCGTGAGTGCTTCGAGCCAGGGTGGCAAGGCCAGTATCCTCATCGAGCCGACCGGCATG
TACGACTACCTGGTCTACCAGGCCGACAATCGACTGACCGTCAGCGTCAAGGCGAAGGCCCAGGAGAGTAATGTCAGGCC
CGGTGCCGATCGCTTCGCGTATACCGGTGAAAAGCTGTCGCTGAATTTCCAGGATATCGATATCCGTTCGGTTCTGCAGT
TGATCGCCGACTTCACCAATCTCAACCTGGTGGCCAGCGACACCGTGCAGGGCAATATCACCCTTCGCCTGCAGAATGTG
CCTTGGGATCAGGCGCTCGACCTGGTGCTCAAGACCAAGGGGCTGGACAAGCGCAAGATCGGCAACGTGCTTCTGGTGGC
GCCGGCCGACGAAATCGCGGCACGCGAGCGTCAAGAGTTGGAGGCGCAACAGCAGATCGCCGAGCTGGCTCCCCTCCGTC
GCGAGCTGGTTCAGGTGAACTATGCCAAAGCGGCGGACATCGCCAAGCTCTTCCAGTCGGTCACCAGCGGCGAAGAGGTT
GGAGAGCAGCGTGGCTCCATCACGGTCGACGAGCGGACCAACAACATCATTGCCTACCAGACCCAGGATCGCCTGGACGA
GTTGCGGCGGATCGTCGCCCAACTTGATATCCCGGTGCGCCAGGTGATGATCGAGGCGCGCATCGTCGAGGCCAATGTCG
ATTACGACAAGAGCTTGGGGGTGCGCTGGGGCGGAAGTTTCGTGAACGACCAGTGGAGCCTGTATGGCAAGGATGGCGCA
CAATCGATCGATGACGGGCAGGTGTTCCTGCCGGGGACCGACAAAGTCGGCAACTTCACGTTGGAGGATGGCGTGGCTCC
CGTCCCCTTCGTCGACATGGGGGTTGCGAACAGCACCTCCGGTCTTGGCATTGGCTTCGTCTCCGATCATCTGATCCTCG
ATCTGCAGCTGTCGGCCATGGAGAAGACCGGCAACGGCGAGGTCATCTCCCAGCCCAAGGTGCTCACCTCGGATAAGGAG
ACGGCAAAGATTCTGACCGGCGAGGAGATCCCCTATCAGGAGGCCAGCTCCAGCGGTGCGACCAGCACCTCCTTCAAGGA
GGCGGCGCTGTCGCTGGAGGTGACGCCGCAGATCACTCCCGACAACCGCATCATCATGGAAGTGAAGGTGACCAAGGATC
AGGCGGACTTCTCCAAGGCCCTGAACGGGGTGCCGCCGATCAACAAGAACGAGGTCAATGCCAAGGTGCTGATCGGCGAC
GGCGAAACCATCGTGATCGGCGGGGTCTTCTCCAATACCCAGGCCAAATCCGTGGACAAGGTGCCTTTCCTCGGCGATTT
GCCGTTCATCGGGCGTCTGTTCCGCCGCGACGTGGTTGCCGACAGCAAATCCGAGCTGCTTGTTTTCCTGACTCCGCGTA
TTCTTAATAACCAGGCTATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(38-121)

Bacterial type II and III secretion system protein (Secretin)

(539-695)

Bacterial type II/III secretion system short domain (Secretin_N)

(376-441)

Secretin and TonB N terminus short domain (STN)

(302-348)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

75.35

100

0.764
  comQ Acinetobacter baylyi ADP1

42.693

99.148

0.423
  pilQ Acinetobacter baumannii strain A118

41.418

100

0.415
  pilQ Acinetobacter baumannii D1279779

41.418

100

0.415
  pilQ Legionella pneumophila strain ERS1305867

37.666

96.165

0.362

Multiple sequence alignment