Detailed information NTG0393274

Bioinformatically predicted

Overview

• Name: comM
• Type: Machinery gene
• Locus tag: GCU53_RS09355
• Genome accession: NZ_CP045302
• Coordinates: 1997191..1998255 (-)
• Length: 354 a.a.
• NCBI ID: WP_152387373.1
• Uniprot ID: -
• Organism: Azotobacter salinestris strain KACC 13899
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1992191..2003255

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GCU53_RS26010	aroK	1992702..1993220 (-)	519	WP_152387368.1	shikimate kinase AroK	-
GCU53_RS09335	pilQ	1993225..1995339 (-)	2115	WP_167520048.1	type IV pilus secretin PilQ	Machinery gene
GCU53_RS09340	pilP	1995393..1995917 (-)	525	WP_152387370.1	type 4a pilus biogenesis lipoprotein PilP	-
GCU53_RS09345	pilO	1995914..1996537 (-)	624	WP_152387371.1	type 4a pilus biogenesis protein PilO	-
GCU53_RS09350	-	1996534..1997190 (-)	657	WP_152387372.1	PilN domain-containing protein	-
GCU53_RS09355	comM	1997191..1998255 (-)	1065	WP_152387373.1	pilus assembly protein PilM	Machinery gene
GCU53_RS09360	-	1998436..2000901 (+)	2466	WP_208845464.1	penicillin-binding protein 1A	-
GCU53_RS09365	-	2001024..2001374 (-)	351	WP_152387375.1	transposase domain-containing protein	-
GCU53_RS09370	-	2001471..2002739 (-)	1269	WP_152387376.1	malic enzyme-like NAD(P)-binding protein	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37631.32 Da
• Isoelectric Point: 4.7417

>NTDB_id=393274 GCU53_RS09355 WP_152387373.1 1997191..1998255(-) (comM) [Azotobacter salinestris strain KACC 13899]
MLGLFNKKAGSLLGIDISSTTVKLIELSRSGNRYRVEAYAVEPLPANAVVEKNIADLDGVGQTLSKVLAKAKTGTKNAAV
AVAGSVVITKTVEMDAGLSEEEMETQLKLEADQYIPYPLEEVAIDFEVQGVLPRSPGRVEVLLAACRKENVEAREAVLAL
AGLTAKVVDVEAYAMERACGLLVNQLGGDPAGLIVAVVDIGATMMTLSVLHDGRTLYTREQLFGGRQLTDEIQRRYGLSV
AEAGLAKKKGGLPEDYDSEVLQPFKEAVVQQVSRSLQFFFAAGQHNQVDYILLAGGTSSLPGLDQLIQQKTGTKTLVANP
FAEMTLSPKVSAAALASDAPAMMVACGLAMRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=393274 GCU53_RS09355 WP_152387373.1 1997191..1998255(-) (comM) [Azotobacter salinestris strain KACC 13899]
GTGCTAGGGCTCTTCAACAAGAAAGCGGGTTCATTACTGGGGATCGATATTAGTTCGACCACGGTCAAGCTTATCGAATT
GAGTCGTTCGGGTAATCGTTACCGGGTGGAGGCCTATGCCGTCGAGCCGCTTCCTGCCAATGCCGTCGTCGAGAAGAATA
TCGCCGATCTCGATGGTGTCGGCCAGACCTTGAGCAAGGTACTGGCCAAGGCCAAGACCGGAACCAAGAATGCAGCGGTG
GCCGTGGCCGGCTCCGTAGTGATCACCAAGACTGTCGAGATGGATGCCGGGCTTTCCGAAGAGGAAATGGAAACCCAGCT
GAAGCTTGAGGCCGACCAATACATTCCCTATCCCCTCGAGGAAGTGGCGATCGACTTCGAAGTCCAGGGGGTGCTGCCGC
GCAGCCCCGGCCGGGTCGAGGTGCTGCTCGCCGCCTGTCGCAAGGAAAACGTCGAAGCCCGCGAGGCAGTGCTGGCACTG
GCCGGCCTGACGGCCAAGGTCGTTGATGTCGAGGCTTATGCCATGGAGCGTGCGTGCGGGCTGCTGGTCAATCAACTGGG
CGGCGATCCGGCGGGGCTGATCGTGGCCGTCGTGGATATCGGCGCGACCATGATGACGCTCAGCGTATTGCATGACGGTC
GCACCCTCTATACCCGTGAGCAGTTGTTTGGCGGCAGGCAGCTCACCGACGAAATCCAGCGCCGTTATGGGCTGTCCGTG
GCCGAAGCCGGTCTGGCCAAGAAAAAAGGCGGACTTCCGGAAGACTACGACAGTGAGGTTCTGCAGCCGTTCAAGGAAGC
CGTCGTACAGCAGGTGTCCCGTTCGCTGCAGTTCTTTTTTGCGGCGGGCCAGCACAACCAGGTCGACTACATCCTGCTGG
CGGGTGGGACCTCTTCCCTGCCGGGGCTTGATCAGTTGATCCAGCAGAAGACCGGTACCAAGACCCTGGTGGCCAATCCG
TTTGCCGAGATGACGCTGAGCCCCAAGGTGAGTGCAGCGGCTCTGGCCAGCGACGCTCCGGCCATGATGGTTGCCTGCGG
TCTGGCGATGAGGAGTTTCGATTAG

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comM	Acinetobacter nosocomialis M2	53.521	100	0.537
pilM	Acinetobacter baumannii D1279779	53.239	100	0.534
comM	Acinetobacter baylyi ADP1	51.831	100	0.52
pilM	Legionella pneumophila strain ERS1305867	45.763	100	0.458