Detailed information NTG0393267

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: GCU53_RS07595
• Genome accession: NZ_CP045302
• Coordinates: 1611165..1612865 (+)
• Length: 566 a.a.
• NCBI ID: WP_152387085.1
• Uniprot ID: -
• Organism: Azotobacter salinestris strain KACC 13899
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1606165..1617865

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GCU53_RS07570	ampE	1606701..1607540 (-)	840	WP_152387080.1	regulatory signaling modulator protein AmpE	-
GCU53_RS07575	ampD	1607537..1608100 (-)	564	WP_152387081.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
GCU53_RS07580	nadC	1608254..1609102 (+)	849	WP_152387082.1	carboxylating nicotinate-nucleotide diphosphorylase	-
GCU53_RS07585	-	1609230..1609931 (-)	702	WP_152387083.1	hypothetical protein	-
GCU53_RS07590	-	1610076..1610615 (-)	540	WP_152387084.1	pilin	-
GCU53_RS07595	pilB	1611165..1612865 (+)	1701	WP_152387085.1	type IV-A pilus assembly ATPase PilB	Machinery gene
GCU53_RS07600	pilC	1612868..1614085 (+)	1218	WP_152387086.1	type II secretion system F family protein	Machinery gene
GCU53_RS07605	pilD	1614088..1614957 (+)	870	WP_152387087.1	A24 family peptidase	Machinery gene
GCU53_RS07610	coaE	1615105..1615713 (+)	609	WP_152387088.1	dephospho-CoA kinase	-
GCU53_RS07615	yacG	1615710..1615910 (+)	201	WP_152387089.1	DNA gyrase inhibitor YacG	-
GCU53_RS07620	-	1616005..1616691 (-)	687	WP_152387090.1	energy-coupling factor ABC transporter permease	-
GCU53_RS07625	-	1616688..1617155 (-)	468	WP_152387091.1	FAD/FMN-containing dehydrogenase	-
GCU53_RS07630	-	1617152..1617577 (-)	426	WP_152387092.1	GNAT family N-acetyltransferase	-

Sequence

Protein

• Length: 566 a.a.
• Molecular weight: 62589.67 Da
• Isoelectric Point: 5.6466

>NTDB_id=393267 GCU53_RS07595 WP_152387085.1 1611165..1612865(+) (pilB) [Azotobacter salinestris strain KACC 13899]
MTNDISLPGLARQMVLAGLIDEKTAQQAQQQAQRNQTSLITWLVQNKLAKSRSLAELAAEQFGIALFDLKTLDRESQPRE
LVSEKLIRQHRVLPLWRRGNRLFVAISDPTNHEAIREIRFGTGLNTEAILVEDDRLGEAIEKYFEGAGTGLDNLADADLD
GLDVEAGDQQDDEINPAGDSEDAPVVRFINKMLLDAIRRGSSDLHFEPYEKSYRVRFRTDGILHEVARPPVQLAPKIAAR
LKVMAGLDISERRKPQDGRIKMKLSKTKAIDFRVNTLPTLWGEKTVMRILDPSSAQMGIDALGYEEVQKELYLAALNQPQ
GMILVTGPTGSGKTVSLYTGLNILNTPEVNISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDIIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPSFNIATSVNLIIAQRLARKLCACKKAVDIPRETLLAEGFPE
ARIGAFKLYAPVGCENCNGGYKGRVGIYEVVKITPALQRIIMEDGNSIEIARQMRADGFNDLRTSALLKAMQGVTSLEEV
NRVTKD

Nucleotide

• Length: 1701 bp

>NTDB_id=393267 GCU53_RS07595 WP_152387085.1 1611165..1612865(+) (pilB) [Azotobacter salinestris strain KACC 13899]
ATGACCAACGATATTTCCCTCCCCGGCCTGGCACGGCAGATGGTGCTGGCCGGGCTGATCGACGAGAAGACCGCCCAGCA
GGCCCAGCAGCAGGCGCAGCGCAACCAGACCTCCCTGATCACCTGGCTGGTGCAGAACAAGCTGGCCAAGAGCCGATCGC
TGGCAGAGCTGGCCGCGGAACAGTTCGGCATCGCCCTGTTCGACCTCAAAACGCTGGACAGGGAGAGCCAGCCCCGGGAA
CTGGTCAGCGAGAAGCTGATTCGCCAGCATCGCGTCCTGCCGCTGTGGCGGCGCGGCAATCGGCTGTTCGTGGCGATCTC
CGACCCGACCAACCACGAGGCGATACGCGAGATCCGCTTCGGCACCGGACTGAACACCGAGGCCATCCTGGTCGAGGACG
ACCGGCTGGGCGAGGCCATCGAGAAATACTTCGAGGGCGCCGGTACCGGCCTGGATAATCTCGCCGATGCCGATCTGGAT
GGGCTCGATGTCGAGGCGGGCGATCAGCAGGACGATGAAATCAATCCGGCCGGCGACTCCGAGGATGCGCCGGTGGTGCG
CTTCATCAACAAGATGCTGCTGGATGCTATTCGTCGCGGCTCCTCGGACCTGCATTTCGAGCCCTACGAAAAAAGCTACC
GGGTGCGCTTCCGTACCGACGGCATCCTCCACGAGGTGGCCCGACCGCCGGTCCAGCTGGCCCCGAAGATCGCCGCGCGG
CTGAAGGTGATGGCCGGGCTGGACATCTCCGAGCGGCGCAAGCCGCAGGATGGCCGGATCAAGATGAAGCTGTCGAAGAC
CAAGGCCATCGACTTTCGGGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGACCGTGATGCGGATTCTCGACCCCTCCA
GCGCGCAGATGGGCATCGATGCCCTCGGCTACGAAGAGGTGCAGAAGGAGCTCTACCTGGCGGCGCTGAACCAGCCGCAG
GGCATGATTCTGGTGACCGGTCCGACCGGCTCGGGCAAGACGGTGTCCCTGTACACCGGCCTGAACATTCTCAACACCCC
GGAGGTGAATATCTCCACCGCGGAAGACCCGGTGGAGATCAACCTGGAGGGCATCAACCAGGTCAACGTCAATCCGCGCC
AGGGCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGACATCATAATGGTCGGCGAGATCCGCGAC
CTGGAAACCGCCGAGATCGCCATCAAGGCCGCGCAGACCGGGCACATGGTGATGTCCACCCTGCACACCAACAGTGCTGC
GGAAACCCTGACTCGCCTGCGCAACATGGGGGTACCCTCCTTCAACATCGCCACCTCGGTGAACCTGATCATCGCCCAGC
GCCTGGCACGCAAGCTGTGCGCCTGCAAGAAGGCAGTGGACATCCCCCGCGAAACCCTGCTCGCCGAAGGCTTCCCGGAA
GCGCGCATCGGCGCCTTCAAGCTGTACGCCCCGGTCGGCTGCGAGAACTGCAACGGCGGCTACAAGGGCCGGGTCGGCAT
TTATGAAGTGGTTAAAATCACCCCTGCCCTGCAGCGCATTATCATGGAGGACGGCAACTCCATCGAAATCGCCCGGCAGA
TGCGCGCCGATGGCTTCAACGACCTGCGCACATCGGCCCTGCTGAAAGCCATGCAGGGCGTCACCAGCCTGGAAGAAGTC
AACCGCGTCACCAAGGATTAA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (43-146)

• Type II/IV secretion system protein (T2SSE) (191-458)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baumannii D1279779	61.21	99.293	0.608
pilB	Acinetobacter baylyi ADP1	58.97	99.47	0.587
pilB	Legionella pneumophila strain ERS1305867	55.088	100	0.555
pilB	Vibrio cholerae strain A1552	51.25	98.94	0.507
pilB	Vibrio parahaemolyticus RIMD 2210633	50.362	97.527	0.491
pilB	Vibrio campbellii strain DS40M4	49.91	97.703	0.488
pilF	Neisseria gonorrhoeae MS11	48.845	99.47	0.486
pilB/pilB1	Synechocystis sp. PCC 6803	38.111	100	0.413
pilF	Thermus thermophilus HB27	37.746	98.763	0.373
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.13	93.463	0.366