Detailed information NTG0393269

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: GCU53_RS07605
• Genome accession: NZ_CP045302
• Coordinates: 1614088..1614957 (+)
• Length: 289 a.a.
• NCBI ID: WP_152387087.1
• Uniprot ID: -
• Organism: Azotobacter salinestris strain KACC 13899
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1609088..1619957

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GCU53_RS07585	-	1609230..1609931 (-)	702	WP_152387083.1	hypothetical protein	-
GCU53_RS07590	-	1610076..1610615 (-)	540	WP_152387084.1	pilin	-
GCU53_RS07595	pilB	1611165..1612865 (+)	1701	WP_152387085.1	type IV-A pilus assembly ATPase PilB	Machinery gene
GCU53_RS07600	pilC	1612868..1614085 (+)	1218	WP_152387086.1	type II secretion system F family protein	Machinery gene
GCU53_RS07605	pilD	1614088..1614957 (+)	870	WP_152387087.1	A24 family peptidase	Machinery gene
GCU53_RS07610	coaE	1615105..1615713 (+)	609	WP_152387088.1	dephospho-CoA kinase	-
GCU53_RS07615	yacG	1615710..1615910 (+)	201	WP_152387089.1	DNA gyrase inhibitor YacG	-
GCU53_RS07620	-	1616005..1616691 (-)	687	WP_152387090.1	energy-coupling factor ABC transporter permease	-
GCU53_RS07625	-	1616688..1617155 (-)	468	WP_152387091.1	FAD/FMN-containing dehydrogenase	-
GCU53_RS07630	-	1617152..1617577 (-)	426	WP_152387092.1	GNAT family N-acetyltransferase	-
GCU53_RS07635	-	1617892..1618065 (+)	174	WP_152387093.1	DUF3094 family protein	-
GCU53_RS07640	-	1618141..1619439 (+)	1299	WP_152387094.1	NAD(P)/FAD-dependent oxidoreductase	-

Sequence

Protein

• Length: 289 a.a.
• Molecular weight: 31617.57 Da
• Isoelectric Point: 6.5724

>NTDB_id=393269 GCU53_RS07605 WP_152387087.1 1614088..1614957(+) (pilD) [Azotobacter salinestris strain KACC 13899]
MSIHDLLAGPGPAFVFCAGLLGLVVGSFLNVVIHRLPKMMLHDWQAQAREVLELPGEEPGQPFNLLLPHSHCPGCGHEIR
PWENIPLLGWLFLGGRCSACKTPISVRYPLVELACGLLSAHVAWHYGFGWQAGAMLLLGWGLLAMSLIDYDHQLLPDSLV
LPLLWLGLILNSFGLFVFLEDALWGAVAGYLSLWSVYWLFKLLTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AVLGILILRLRRAESGTPIPFGPFLALAGWIALLWGERITGSYLQFAGL

Nucleotide

• Length: 870 bp

>NTDB_id=393269 GCU53_RS07605 WP_152387087.1 1614088..1614957(+) (pilD) [Azotobacter salinestris strain KACC 13899]
ATGTCCATTCACGATCTTCTGGCCGGTCCCGGACCGGCCTTCGTTTTCTGTGCGGGGCTGCTGGGCCTGGTGGTCGGCAG
CTTTCTCAACGTGGTCATCCATCGCCTGCCCAAGATGATGCTGCACGATTGGCAGGCGCAGGCGCGGGAAGTGCTGGAGC
TGCCCGGCGAGGAGCCGGGCCAGCCCTTCAACCTGCTGCTGCCCCACTCCCACTGCCCAGGCTGCGGGCACGAAATCAGG
CCCTGGGAGAATATCCCGCTGCTCGGCTGGCTATTCCTGGGAGGCCGCTGTTCGGCCTGCAAGACGCCGATCAGCGTGCG
CTATCCCCTAGTGGAGCTGGCCTGCGGACTGCTTTCGGCCCACGTTGCCTGGCATTACGGCTTCGGCTGGCAGGCCGGTG
CCATGCTGCTGCTCGGCTGGGGCCTGCTGGCCATGAGCCTGATCGACTACGACCATCAACTGCTACCGGACTCGCTGGTG
CTGCCACTGCTATGGCTGGGTCTGATCCTCAACAGTTTCGGATTGTTCGTCTTCCTCGAGGATGCCCTCTGGGGCGCGGT
GGCCGGCTACCTCAGCCTGTGGTCGGTGTACTGGCTGTTCAAGCTGCTCACCGGCAAGGAGGGCATGGGCTACGGCGACT
TCAAGCTGCTGGCGATGCTCGGCGCCTGGGGCGGCTGGCAGATCCTGCCGCTCACCATCCTGCTCTCCTCGCTGGTCGGC
GCCGTGCTGGGGATCCTCATCCTGCGTCTGCGCAGGGCCGAAAGCGGCACGCCGATCCCCTTCGGCCCCTTCCTCGCCCT
CGCCGGCTGGATCGCCCTGCTCTGGGGCGAGCGCATCACCGGCTCCTACCTGCAGTTCGCCGGCTTGTAG

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

• Type IV leader peptidase family (Peptidase_A24) (137-245)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	54.745	94.81	0.519
pilD	Vibrio campbellii strain DS40M4	51.648	94.464	0.488
pilD	Acinetobacter nosocomialis M2	49.091	95.156	0.467
pilD	Acinetobacter baumannii D1279779	49.091	95.156	0.467
pilD	Neisseria gonorrhoeae MS11	49.259	93.426	0.46