Detailed information NTG0393264

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: GCU53_RS02480
• Genome accession: NZ_CP045302
• Coordinates: 523187..524344 (-)
• Length: 385 a.a.
• NCBI ID: WP_208845433.1
• Uniprot ID: -
• Organism: Azotobacter salinestris strain KACC 13899
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 518187..529344

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GCU53_RS02460	-	518234..519934 (-)	1701	WP_244307130.1	autotransporter assembly complex family protein	-
GCU53_RS02465	-	520034..520672 (-)	639	WP_152386213.1	GNAT family N-acetyltransferase	-
GCU53_RS02470	xthA	520809..521621 (+)	813	WP_152386214.1	exodeoxyribonuclease III	-
GCU53_RS02475	-	521755..523134 (+)	1380	WP_152386215.1	phosphate ABC transporter substrate-binding/OmpA family protein	-
GCU53_RS02480	pilU	523187..524344 (-)	1158	WP_208845433.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
GCU53_RS02485	-	524467..524745 (+)	279	WP_152386216.1	peptidylprolyl isomerase	-
GCU53_RS02490	-	524953..526182 (+)	1230	WP_152386217.1	MFS transporter	-
GCU53_RS02495	-	526204..526431 (-)	228	WP_152386218.1	hypothetical protein	-
GCU53_RS02500	-	526522..527619 (+)	1098	WP_152386219.1	IS630 family transposase	-
GCU53_RS02505	-	527781..528761 (-)	981	WP_152385958.1	IS5 family transposase	-

Sequence

Protein

• Length: 385 a.a.
• Molecular weight: 42618.79 Da
• Isoelectric Point: 6.3261

>NTDB_id=393264 GCU53_RS02480 WP_208845433.1 523187..524344(-) (pilU) [Azotobacter salinestris strain KACC 13899]
MDIQSMLRVLATQDGSDLYLSTGAPPCAKFNGVLKALSQEVLQPGAVAKIAEGLMDAEQRIEFERDLEMNLAISLPNIGR
FRINIFKQRNEVSIVARNIKLDIPRFEDLKLPKILLDVVMEKRGLVLFVGGTGSGKSTSLAALIDYRNRNSGGHIITIED
PVEYVHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTVDGKRRAAVEVLLGTPTVRDLIKRSELSEIKEIMDKSRNLGMQTFDQAL
IELVNEGAISEEEALKNADSANNVRLKLKLHRDGGAGAADKPDSESAWPGLSLEMLEEPEEAGKA

Nucleotide

• Length: 1158 bp

>NTDB_id=393264 GCU53_RS02480 WP_208845433.1 523187..524344(-) (pilU) [Azotobacter salinestris strain KACC 13899]
ATGGATATTCAATCGATGCTGAGAGTCCTGGCTACCCAGGACGGTTCGGACCTTTACCTGTCCACCGGCGCGCCGCCCTG
CGCGAAATTCAACGGCGTGCTCAAGGCGCTCTCCCAGGAGGTGCTGCAGCCGGGCGCCGTGGCGAAGATCGCCGAGGGAC
TGATGGATGCCGAACAGCGCATCGAATTCGAGCGCGACCTGGAGATGAACCTGGCGATCTCCCTGCCGAACATCGGCCGC
TTCCGCATCAACATCTTCAAGCAGCGCAACGAGGTGTCCATCGTCGCGCGCAACATCAAGCTGGACATCCCGCGCTTCGA
GGACCTCAAGCTGCCGAAGATCCTCCTCGACGTGGTGATGGAGAAGCGTGGCCTGGTGCTGTTCGTCGGCGGCACCGGCT
CGGGCAAGTCGACCTCCCTGGCGGCGCTGATCGACTACCGCAACCGCAACAGCGGCGGGCACATCATCACCATCGAGGAC
CCGGTCGAATACGTGCACCGGCACAAGAAGTCGATCATCAACCAGCGCGAGGTCGGGGTGGACACCAGAAGCTTCCACGC
CGCGCTGAAGAACACCCTGCGCCAGGCGCCGGATGTGATCCTGATCGGCGAGATCCGCGACCGCGAGACCATGGAGCATG
CTCTGGCCTTCGCCGACACCGGCCACCTGGCGATCTCCACGCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTTCCCGAGGAGCGCCGTCCGCAGCTGCTCAACGACCTGGGCAACAACCTCAAGGCCTTCGTCTCCCAGCG
CCTGGTCAAGACGGTCGACGGCAAGCGGCGGGCCGCGGTGGAGGTGCTGCTCGGCACGCCGACGGTGCGCGATCTGATCA
AGCGCAGCGAGCTGTCCGAGATCAAGGAGATCATGGACAAGTCGCGCAACCTCGGCATGCAGACCTTCGACCAGGCACTG
ATCGAGCTGGTCAACGAGGGGGCGATCAGCGAGGAGGAGGCGCTGAAGAACGCCGACTCGGCGAACAACGTGCGCCTCAA
GCTGAAACTGCACCGTGACGGCGGGGCGGGCGCTGCCGACAAGCCGGATAGCGAGAGTGCCTGGCCGGGGCTGAGCCTGG
AGATGCTGGAAGAGCCGGAGGAGGCCGGCAAGGCCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (112-248)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	54.521	97.662	0.532
pilU	Acinetobacter baylyi ADP1	51.648	94.545	0.488
pilU	Vibrio cholerae strain A1552	51.247	93.766	0.481
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.478	88.052	0.374
pilT	Legionella pneumophila strain Lp02	42.515	86.753	0.369
pilT	Legionella pneumophila strain ERS1305867	42.515	86.753	0.369
pilT	Pseudomonas aeruginosa PAK	40.525	89.091	0.361