Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GA660_RS12830 Genome accession   NZ_CP045129
Coordinates   2694069..2695775 (+) Length   568 a.a.
NCBI ID   WP_075167609.1    Uniprot ID   A0AAW8Z0C9
Organism   Acinetobacter indicus strain TQ04     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2689069..2700775
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GA660_RS12790 nusA 2689736..2691220 (-) 1485 WP_005181786.1 transcription termination factor NusA -
  GA660_RS12795 rimP 2691253..2691777 (-) 525 WP_005181784.1 ribosome maturation factor RimP -
  GA660_RS12820 secG 2692646..2692975 (-) 330 WP_005181783.1 preprotein translocase subunit SecG -
  GA660_RS12825 tpiA 2692989..2693780 (-) 792 WP_005181780.1 triose-phosphate isomerase -
  GA660_RS12830 pilB 2694069..2695775 (+) 1707 WP_075167609.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GA660_RS12835 pilC 2695806..2697032 (+) 1227 WP_160241680.1 type II secretion system F family protein Machinery gene
  GA660_RS12840 pilD 2697032..2697892 (+) 861 WP_005181774.1 prepilin peptidase Machinery gene
  GA660_RS12845 coaE 2697903..2698511 (+) 609 WP_075175011.1 dephospho-CoA kinase -
  GA660_RS12850 - 2698508..2699428 (-) 921 WP_016659657.1 DMT family transporter -
  GA660_RS12855 rlmB 2699446..2700198 (-) 753 WP_005181769.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  GA660_RS12860 - 2700304..2700627 (-) 324 WP_075167606.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 63787.16 Da        Isoelectric Point: 4.8301

>NTDB_id=392144 GA660_RS12830 WP_075167609.1 2694069..2695775(+) (pilB) [Acinetobacter indicus strain TQ04]
MSVQQAMPRFTGFIRRLVEEGLVTAENMQQAIVAARKAETDIVPYLIQQLQISPLTIAEKISYEFGEPIFDLAVYDSRLI
VREGLDEKLFTKYRILPIFRRHNQLYIATSNPTNIEAIDAIRFNSKLNIEVVIVEHDKLERLIEQNFTEDSTFDFDEDFD
LEVGDNAPETQEDDDEPQGDEAPIVKYINKLLVDAIRMGASDLHFEPYEKSYRVRYRIDGVLRQIANPPLQLATRLASRL
KVMSQMDISEKRIPQDGRIKLKLSKNKAIDFRVNSLPTLFGEKIVLRILDPSSAMLGIDALGYEPEQKELFMEALDKPQG
MLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLEGINQVNVNPKVGLTFAAALKSFLRQDPDIVMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCQKCKAPVEIPRQSLLELGFTE
EDLNQPEFQLYQPVGCPECREGYKGRVGVYEVMKVTPEISRIIMEDGNALQIAAASAKSGFNNLRRSGLIKVMQGVTSLQ
EVNRVTSE

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=392144 GA660_RS12830 WP_075167609.1 2694069..2695775(+) (pilB) [Acinetobacter indicus strain TQ04]
ATGTCAGTACAACAAGCAATGCCTAGATTTACAGGTTTTATTCGGCGACTGGTCGAAGAAGGGCTTGTGACGGCTGAAAA
TATGCAGCAAGCCATTGTCGCGGCAAGAAAAGCTGAGACCGATATTGTGCCGTATTTAATTCAGCAGTTGCAGATTTCAC
CGCTGACTATTGCTGAAAAAATTTCCTATGAATTTGGTGAGCCGATTTTTGACTTAGCCGTGTACGATTCGCGTCTGATT
GTACGTGAAGGTCTGGATGAAAAACTGTTCACCAAATACCGAATTCTGCCCATTTTCCGACGTCACAATCAGCTTTATAT
TGCTACCAGTAACCCAACCAATATCGAAGCCATTGATGCGATCCGCTTTAACAGCAAGCTGAATATTGAAGTGGTGATTG
TTGAGCATGACAAGCTGGAACGGCTGATTGAACAGAACTTTACCGAAGACAGCACCTTTGACTTTGATGAAGACTTTGAT
CTGGAAGTTGGCGACAACGCTCCCGAAACACAAGAAGATGACGATGAGCCACAAGGCGATGAAGCCCCAATCGTCAAATA
TATTAATAAGTTACTGGTCGATGCAATCCGTATGGGCGCCTCGGATTTGCACTTCGAGCCTTATGAAAAAAGCTACCGGG
TCCGTTACCGGATTGACGGCGTTTTGCGCCAGATTGCCAATCCTCCGTTACAGCTGGCCACACGCCTCGCCTCACGTTTA
AAAGTTATGTCACAAATGGACATCTCAGAAAAACGTATTCCGCAGGATGGCCGGATCAAGCTCAAGCTGTCAAAAAACAA
AGCCATCGACTTCCGTGTCAACTCATTGCCGACCCTGTTTGGAGAAAAAATTGTACTACGTATTCTCGATCCATCTAGCG
CCATGCTGGGGATTGATGCACTTGGTTATGAACCGGAGCAAAAAGAGCTGTTTATGGAGGCGCTGGACAAACCACAAGGC
ATGTTACTGATTACCGGTCCGACCGGTTCCGGTAAAACCGTGTCTTTATATACCGGCTTAAATATCCTCAACCGCGAAGA
CACCAATATTTCTACCGCCGAAGATCCGGTGGAAATTAACCTGGAAGGGATTAATCAGGTCAACGTCAATCCGAAAGTGG
GTCTGACCTTTGCCGCAGCACTAAAATCATTCCTGCGCCAGGATCCGGATATTGTTATGGTCGGTGAGATCCGTGATCTG
GAAACTGCAGAAATTGCTATTAAAGCTGCACAAACCGGTCACATGGTCATGTCGACGCTGCACACCAACAGTGCACCCGA
AACCCTGACCCGTTTACGCAACATGGGCGTCCCTTCATTTAACATCGCCACCTCGGTGAACCTGGTGATTGCCCAGCGTC
TGGCACGTCGTCTGTGTCAGAAATGCAAGGCCCCGGTGGAGATTCCACGCCAGAGCCTCTTGGAGCTCGGCTTTACCGAA
GAAGATTTAAACCAGCCAGAGTTCCAGCTGTATCAGCCGGTTGGCTGCCCGGAATGTCGTGAAGGTTATAAAGGCCGTGT
CGGGGTCTATGAAGTGATGAAAGTCACCCCGGAAATCTCACGCATTATTATGGAAGATGGCAATGCCTTGCAGATTGCAG
CAGCCTCGGCAAAATCCGGGTTTAACAATTTACGCCGTTCAGGATTAATCAAGGTCATGCAGGGGGTCACTTCTTTACAG
GAAGTGAATCGTGTGACCAGCGAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-147)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

80.351

100

0.806
  pilB Acinetobacter baylyi ADP1

77.895

100

0.782
  pilB Legionella pneumophila strain ERS1305867

50.97

99.824

0.509
  pilF Neisseria gonorrhoeae MS11

51.429

98.592

0.507
  pilB Vibrio cholerae strain A1552

49.647

99.648

0.495
  pilB Vibrio parahaemolyticus RIMD 2210633

49.372

98.063

0.484
  pilB Vibrio campbellii strain DS40M4

50.864

91.725

0.467
  pilF Thermus thermophilus HB27

37.434

100

0.375
  pilB/pilB1 Synechocystis sp. PCC 6803

33.659

100

0.364

Multiple sequence alignment