Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   GA645_RS25855 Genome accession   NZ_CP045118
Coordinates   5662005..5664143 (-) Length   712 a.a.
NCBI ID   WP_152226778.1    Uniprot ID   -
Organism   Pseudomonas sp. SCB32     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5657005..5669143
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GA645_RS25840 - 5658614..5660314 (-) 1701 WP_152226774.1 AAA family ATPase -
  GA645_RS25845 aroB 5660321..5661427 (-) 1107 WP_152226776.1 3-dehydroquinate synthase -
  GA645_RS25850 aroK 5661478..5661969 (-) 492 WP_372239817.1 shikimate kinase AroK -
  GA645_RS25855 pilQ 5662005..5664143 (-) 2139 WP_152226778.1 type IV pilus secretin PilQ Machinery gene
  GA645_RS25860 pilP 5664197..5664721 (-) 525 WP_152226780.1 type 4a pilus biogenesis lipoprotein PilP -
  GA645_RS25865 pilO 5664718..5665368 (-) 651 WP_152226782.1 type 4a pilus biogenesis protein PilO -
  GA645_RS25870 pilN 5665365..5665958 (-) 594 WP_152226784.1 type 4a pilus biogenesis protein PilN -
  GA645_RS25875 pilM 5665958..5667022 (-) 1065 WP_152226786.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 712 a.a.        Molecular weight: 76544.98 Da        Isoelectric Point: 5.2915

>NTDB_id=392044 GA645_RS25855 WP_152226778.1 5662005..5664143(-) (pilQ) [Pseudomonas sp. SCB32]
MNKMLSRLSISLLAAVFAPALLAANLQSVDVSALPGDRVELKLGFDGPVAAPRGYTIEQPARIALDLPGVQNKLGSKNRE
LGVGNARSLTVVEAKDRTRLIVNLATLVPYTTRSEGSNLYVVVGGAGASSTVASAAPVSAPIAKPAQSKSYIAAGKAIRN
IDFQRGDQGEGNVVIDLSDPTVSPDIQEQGGKIRLEFPRTQLPDNLRVRLDVKDFATPVQFVNASAAGDKASISIEPTGL
YDYLVYQTDNRMTVSIKPLTQDDAEKRKKDTFAYTGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITLRLQNV
PWDQALDLVLKTKGLDKRKVGNVLLVAPAEEIAARERQELEAQKQISELAPLRRELIQVNYAKAADIAKLFQSVTGVGAP
GAPQTPGADNRGSITVDDRTNAIIAYQTQERLDELRRIVAQLDIPVRQVMIEARIVEAAVDYSKSLGVRWGGAFSSGNWD
FYGKDGARTVDSNGKLFLPGTDTIGNFTAKDGVAPVPFVDMGAVNSTSGIGIGFLSDHAMLDLQLSAMEKSGNGEVVSQP
KVVTSDKETAKILKGSEIPYQEASSSGATSVSFKEAALALEVTPQITPDNRIIMEVKVNKDEPDYANALNGVPPIKKNEV
NAKVLVNDGETIVIGGVFSNTQTKQTDKVPFLGDVPFIGRLFRRDSVTDSKSELLVFLTPRIMNNQAFAAGR

Nucleotide


Download         Length: 2139 bp        

>NTDB_id=392044 GA645_RS25855 WP_152226778.1 5662005..5664143(-) (pilQ) [Pseudomonas sp. SCB32]
ATGAACAAAATGCTTTCCCGCCTGAGCATCTCCTTGCTCGCGGCAGTCTTTGCGCCAGCATTGCTGGCCGCCAATCTGCA
GAGCGTCGATGTTTCAGCCTTGCCGGGTGATCGGGTCGAGCTGAAGCTGGGCTTCGACGGTCCGGTGGCGGCGCCGCGTG
GCTACACCATCGAGCAGCCGGCGCGTATCGCGCTGGATCTGCCGGGTGTGCAGAACAAGCTGGGCAGCAAGAATCGCGAG
CTGGGCGTGGGTAATGCGCGCAGCCTGACGGTGGTTGAAGCAAAAGATCGCACCCGCCTGATCGTCAACCTTGCCACCCT
TGTGCCATACACCACTCGGTCGGAAGGTTCGAACCTGTACGTCGTGGTGGGTGGCGCCGGTGCCTCCAGCACGGTTGCAT
CGGCGGCGCCGGTATCCGCGCCAATCGCCAAGCCGGCACAGTCCAAGTCCTACATTGCGGCGGGCAAGGCAATTCGCAAC
ATCGACTTCCAGCGTGGCGATCAAGGGGAAGGCAATGTCGTGATCGACCTGTCCGATCCGACCGTCAGTCCGGACATTCA
GGAGCAGGGCGGCAAGATCCGCCTGGAATTCCCGCGGACCCAATTGCCTGACAACCTGCGCGTACGCCTGGACGTGAAGG
ACTTCGCCACGCCGGTACAGTTCGTCAACGCTTCCGCGGCGGGCGACAAGGCGAGCATCTCCATCGAGCCGACCGGCCTG
TACGACTATCTCGTTTATCAGACTGATAACCGGATGACCGTCAGTATCAAGCCGCTGACTCAGGATGATGCGGAGAAGCG
CAAGAAGGATACCTTCGCCTACACCGGCGAGAAGTTGTCGCTGAACTTCCAGGACATCGATGTGCGCTCGGTCCTGCAGT
TGATTGCCGACTTCACCGACCTGAACCTGGTGGCTTCAGATACCGTCCAGGGCAATATCACCCTGCGTCTGCAGAACGTG
CCGTGGGACCAGGCGCTGGACCTTGTGCTGAAGACCAAGGGTCTGGACAAGCGCAAGGTGGGCAACGTTCTGCTGGTCGC
GCCGGCTGAAGAGATCGCAGCGCGTGAGCGCCAGGAGCTCGAGGCCCAGAAGCAGATTTCCGAACTGGCTCCGCTTCGTC
GCGAGCTGATTCAGGTGAACTACGCGAAGGCAGCTGACATCGCCAAGCTGTTCCAGTCGGTAACCGGCGTCGGTGCTCCG
GGTGCGCCGCAGACTCCGGGCGCGGACAACCGTGGCTCGATCACTGTCGATGATCGCACCAACGCGATCATCGCCTACCA
GACCCAGGAACGCCTGGATGAGCTTCGTCGTATCGTCGCGCAATTGGACATCCCGGTTCGCCAGGTGATGATCGAGGCGC
GCATCGTCGAAGCCGCCGTCGACTACAGCAAGAGCCTTGGTGTTCGCTGGGGCGGCGCATTCAGCTCCGGCAACTGGGAT
TTCTATGGTAAGGACGGGGCCCGTACTGTCGATAGCAACGGCAAGTTGTTCCTGCCCGGTACCGATACCATTGGTAACTT
TACCGCGAAGGATGGTGTTGCGCCGGTCCCGTTCGTTGACATGGGTGCGGTAAACAGCACCTCGGGTATTGGCATTGGCT
TCCTGAGCGATCACGCCATGCTCGACCTGCAGCTTTCCGCCATGGAAAAGAGCGGCAACGGTGAGGTGGTCTCCCAGCCG
AAGGTCGTTACCTCCGATAAGGAAACCGCGAAGATCCTGAAGGGTTCGGAGATTCCTTACCAGGAAGCCAGCTCCAGCGG
TGCTACCTCGGTGTCCTTCAAGGAGGCCGCCCTGGCCCTGGAAGTGACCCCGCAGATCACCCCGGACAACCGCATCATCA
TGGAAGTGAAGGTCAATAAGGACGAGCCGGACTACGCCAACGCACTCAATGGCGTACCGCCGATCAAGAAGAACGAGGTG
AACGCCAAGGTGCTGGTCAACGATGGCGAGACCATCGTGATCGGCGGTGTGTTCTCCAATACCCAGACCAAGCAGACCGA
CAAGGTTCCGTTCCTGGGTGACGTTCCGTTCATTGGACGGCTGTTCCGTCGTGATTCGGTAACCGACAGCAAGAGCGAGC
TGCTGGTTTTCCTTACCCCGCGGATCATGAATAATCAGGCCTTTGCAGCCGGCCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(302-348)

Bacterial type II and III secretion system protein (Secretin)

(547-703)

Bacterial type II/III secretion system short domain (Secretin_N)

(376-448)

AMIN domain (AMIN)

(39-121)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

78.691

100

0.794
  comQ Acinetobacter baylyi ADP1

41.337

100

0.426
  pilQ Acinetobacter baumannii strain A118

40.217

100

0.416
  pilQ Acinetobacter baumannii D1279779

40.217

100

0.416
  pilQ Legionella pneumophila strain ERS1305867

38.873

97.191

0.378
  pilQ Legionella pneumophila strain Lp02

38.728

97.191

0.376

Multiple sequence alignment