Detailed information NTG0392045

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: GA645_RS25875
• Genome accession: NZ_CP045118
• Coordinates: 5665958..5667022 (-)
• Length: 354 a.a.
• NCBI ID: WP_152226786.1
• Uniprot ID: -
• Organism: Pseudomonas sp. SCB32
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5660958..5672022

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GA645_RS25850	aroK	5661478..5661969 (-)	492	WP_372239817.1	shikimate kinase AroK	-
GA645_RS25855	pilQ	5662005..5664143 (-)	2139	WP_152226778.1	type IV pilus secretin PilQ	Machinery gene
GA645_RS25860	pilP	5664197..5664721 (-)	525	WP_152226780.1	type 4a pilus biogenesis lipoprotein PilP	-
GA645_RS25865	pilO	5664718..5665368 (-)	651	WP_152226782.1	type 4a pilus biogenesis protein PilO	-
GA645_RS25870	pilN	5665365..5665958 (-)	594	WP_152226784.1	type 4a pilus biogenesis protein PilN	-
GA645_RS25875	pilM	5665958..5667022 (-)	1065	WP_152226786.1	pilus assembly protein PilM	Machinery gene
GA645_RS25880	-	5667208..5669646 (+)	2439	WP_152226788.1	penicillin-binding protein 1A	-
GA645_RS25885	-	5669883..5670377 (-)	495	WP_152226790.1	YgjP-like metallopeptidase domain-containing protein	-
GA645_RS25890	-	5670428..5671198 (-)	771	WP_152226792.1	class I SAM-dependent methyltransferase	-
GA645_RS25895	-	5671236..5671655 (-)	420	WP_152226794.1	hypothetical protein	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37775.07 Da
• Isoelectric Point: 4.4486

>NTDB_id=392045 GA645_RS25875 WP_152226786.1 5665958..5667022(-) (pilM) [Pseudomonas sp. SCB32]
MLGLFNKKANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIAELEGVGQALSRVVAKARTGVKTAAV
AVAGSAVITKTIEMDGGLSDDELENQLKIEADQYIPYPLEEVAIDFEVQGVSARNAERVDVLLAACRKENVEVREAALAL
SGLTAKVVDVEAYALERAFSLLGDQLGGNADELTVAVVDIGATMTTLSVLHHGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLQPFKDAVVQQVSRSLQFFFAAGQFNDVDYILLAGGTASIPDLDRLIQQKIGTPTLVANP
FTDMVLSGKVNAGALASDAPALMIACGLAMRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=392045 GA645_RS25875 WP_152226786.1 5665958..5667022(-) (pilM) [Pseudomonas sp. SCB32]
GTGCTAGGGCTATTCAATAAGAAAGCGAACACGCTGCTGGGGATAGACATCAGTTCGACTTCGGTCAAACTCCTCGAACT
GAGCCGCTCGGGAGGCCGCTACAAGGTAGAGGCCTATGCTGTAGAGCCGCTCCCTCCCAATGCAGTGGTAGAAAAGAACA
TCGCTGAGCTGGAGGGCGTCGGCCAGGCGTTGTCACGTGTGGTCGCCAAAGCGCGCACCGGCGTGAAGACGGCTGCGGTC
GCGGTTGCCGGCTCTGCGGTAATCACCAAGACCATCGAGATGGACGGTGGCCTCTCGGACGACGAGCTGGAAAATCAGCT
CAAGATCGAGGCCGACCAGTACATCCCCTATCCCCTCGAAGAAGTCGCGATCGACTTCGAGGTCCAGGGCGTATCCGCGC
GGAATGCCGAGCGCGTTGACGTACTCCTCGCCGCTTGTCGGAAGGAAAACGTTGAAGTGCGCGAGGCGGCCCTGGCGCTG
TCGGGGCTGACGGCCAAGGTTGTCGACGTCGAGGCGTACGCACTGGAGCGAGCATTCAGTCTGCTCGGCGATCAACTGGG
CGGCAATGCCGACGAGCTCACAGTTGCGGTGGTCGACATCGGCGCCACCATGACCACGCTGAGCGTGCTGCACCATGGCC
GCACCATCTATACACGCGAGCAGCTGTTCGGCGGGCGTCAGCTCACCGAGGAGATTCAGCGTCGCTACGGTCTGTCCGTG
GAAGAAGCCGGTCTTGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAAGTTCTGCAACCGTTCAAAGATGC
TGTCGTTCAGCAGGTGTCCCGTTCGCTGCAGTTCTTCTTCGCGGCAGGCCAGTTCAACGACGTGGACTACATCCTGCTGG
CGGGCGGAACGGCCTCCATTCCCGATCTGGACAGGTTGATCCAGCAGAAGATCGGTACTCCGACACTCGTAGCCAACCCC
TTCACCGACATGGTGCTCAGTGGAAAGGTCAATGCGGGTGCGCTGGCCAGTGATGCGCCGGCGCTGATGATTGCCTGCGG
TCTGGCGATGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	57.345	100	0.573
comM	Acinetobacter nosocomialis M2	57.345	100	0.573
comM	Acinetobacter baylyi ADP1	53.39	100	0.534
pilM	Legionella pneumophila strain ERS1305867	46.328	100	0.463