Detailed information NTG0391847

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: F9230_RS01285
• Genome accession: NZ_CP045099
• Coordinates: 290285..291343 (+)
• Length: 352 a.a.
• NCBI ID: WP_058870420.1
• Uniprot ID: A0A345B4L2
• Organism: Acinetobacter johnsonii strain AJ14
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 285285..296343

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
F9230_RS01270 (F9230_01275)	mraY	285565..286683 (+)	1119	WP_058951625.1	phospho-N-acetylmuramoyl-pentapeptide- transferase	-
F9230_RS01275 (F9230_01280)	-	286753..287559 (+)	807	WP_234951046.1	putative RNA methyltransferase	-
F9230_RS01280 (F9230_01285)	ponA	287591..290119 (-)	2529	WP_234951047.1	penicillin-binding protein PBP1a	-
F9230_RS01285 (F9230_01290)	pilM	290285..291343 (+)	1059	WP_058870420.1	pilus assembly protein PilM	Machinery gene
F9230_RS01290 (F9230_01295)	pilN	291343..291999 (+)	657	WP_234951048.1	PilN domain-containing protein	Machinery gene
F9230_RS01295 (F9230_01300)	pilO	291996..292733 (+)	738	WP_234951049.1	type 4a pilus biogenesis protein PilO	Machinery gene
F9230_RS01300 (F9230_01305)	pilP	292733..293260 (+)	528	WP_094148569.1	pilus assembly protein PilP	Machinery gene
F9230_RS01305 (F9230_01310)	pilQ	293280..295358 (+)	2079	WP_184117977.1	type IV pilus secretin PilQ family protein	Machinery gene
F9230_RS01310 (F9230_01315)	aroK	295396..295929 (+)	534	WP_062032260.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 38953.50 Da
• Isoelectric Point: 4.4181

>NTDB_id=391847 F9230_RS01285 WP_058870420.1 290285..291343(+) (pilM) [Acinetobacter johnsonii strain AJ14]
MLRLYRKPSKGLIGVDISSTSVKVLELSVKNNRYWVESYALVPLPEGSVVEKNILNPEAVGDALERAVNLANVQSTDVAL
AVPTSMVITKIIEMDADMNDDEREIQIRDDAEQYIPFPLDEASLDFEVLPDRLANPNRVNVLLVATRIENVEARSEALEL
GGLTPKIADVESFAIENAFKVFSDTLPMGVNTVGILDIGHTMTTLSVMQNNKVIYTREQVFGGKQLTQEIQNRYGLSFEE
AGRAKKSRTLPDDYDIEVLEPFLEAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQIDIKKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=391847 F9230_RS01285 WP_058870420.1 290285..291343(+) (pilM) [Acinetobacter johnsonii strain AJ14]
GTGCTCAGGTTATATCGTAAGCCAAGTAAGGGGTTAATAGGAGTAGATATTAGTTCGACTTCTGTTAAGGTATTGGAACT
TTCTGTTAAAAACAATCGGTATTGGGTGGAGAGCTATGCACTAGTCCCATTACCAGAAGGAAGTGTGGTCGAAAAAAATA
TATTGAATCCAGAAGCGGTTGGTGATGCCCTTGAGCGTGCGGTAAATTTGGCCAATGTTCAATCGACAGACGTAGCACTG
GCAGTTCCGACGTCCATGGTGATTACTAAAATCATTGAAATGGATGCCGATATGAACGATGACGAACGTGAAATTCAGAT
TCGTGATGATGCTGAGCAATATATTCCATTCCCATTAGATGAAGCGAGTTTGGACTTTGAAGTCCTTCCAGACCGTTTAG
CCAATCCCAATCGTGTCAATGTACTCCTCGTTGCAACCCGCATTGAAAATGTAGAGGCGCGTTCCGAAGCATTGGAATTA
GGTGGTTTAACGCCTAAGATTGCCGATGTTGAAAGTTTCGCCATTGAAAATGCATTCAAAGTCTTTTCAGATACGCTTCC
AATGGGTGTAAACACCGTTGGGATTCTGGACATCGGTCATACCATGACGACCTTATCTGTCATGCAGAATAATAAAGTCA
TTTATACGCGTGAACAGGTGTTCGGTGGTAAACAACTGACTCAAGAAATTCAAAACCGTTATGGTTTGTCCTTTGAGGAA
GCTGGACGTGCTAAAAAAAGCCGTACTTTGCCTGATGACTATGATATTGAAGTACTTGAACCTTTTCTTGAAGCTGTGGT
GCAGCAAGCAGCACGTTCTTTGCAGTTCTTTTTTTCGTCCTCTCAATTTAATGAGATCGACCATATTTTATTGGCAGGGG
GGAATGCCAATATTCCAGGACTGGCGAAGTTATTACAACAAAAATTAGGTTATCGGGTCACTATTGCCAACCCATTTTTA
CAGATGGGCTTTTCTCCACAAATCGACATTAAAAAAATTGAAAATGATGCTTCATCACTCATGGTGGCATGTGGATTGGC
ATTGAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: A0A345B4L2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	85.227	100	0.852
comM	Acinetobacter nosocomialis M2	85.227	100	0.852
comM	Acinetobacter baylyi ADP1	80.398	100	0.804
pilM	Legionella pneumophila strain ERS1305867	40.96	100	0.412