Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   F9230_RS01295 Genome accession   NZ_CP045099
Coordinates   291996..292733 (+) Length   245 a.a.
NCBI ID   WP_234951049.1    Uniprot ID   -
Organism   Acinetobacter johnsonii strain AJ14     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 286996..297733
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F9230_RS01280 (F9230_01285) ponA 287591..290119 (-) 2529 WP_234951047.1 penicillin-binding protein PBP1a -
  F9230_RS01285 (F9230_01290) pilM 290285..291343 (+) 1059 WP_058870420.1 pilus assembly protein PilM Machinery gene
  F9230_RS01290 (F9230_01295) pilN 291343..291999 (+) 657 WP_234951048.1 PilN domain-containing protein Machinery gene
  F9230_RS01295 (F9230_01300) pilO 291996..292733 (+) 738 WP_234951049.1 type 4a pilus biogenesis protein PilO Machinery gene
  F9230_RS01300 (F9230_01305) pilP 292733..293260 (+) 528 WP_094148569.1 pilus assembly protein PilP Machinery gene
  F9230_RS01305 (F9230_01310) pilQ 293280..295358 (+) 2079 WP_184117977.1 type IV pilus secretin PilQ family protein Machinery gene
  F9230_RS01310 (F9230_01315) aroK 295396..295929 (+) 534 WP_062032260.1 shikimate kinase AroK -
  F9230_RS01315 (F9230_01320) aroB 295960..297045 (+) 1086 WP_234951050.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 245 a.a.        Molecular weight: 27751.28 Da        Isoelectric Point: 5.1834

>NTDB_id=391849 F9230_RS01295 WP_234951049.1 291996..292733(+) (pilO) [Acinetobacter johnsonii strain AJ14]
MSRDEFEELTSENHAVTKKKMTVDKFFQQFNTLDPNNYGSWPFSVKLTCWIFIILVIGALGYFVVIKPKLDAISNAHAQE
QNLLNEFREKDSKLRNLQQYQAQLQEMEANFNQQLEQLPKETEIPGLVEDINVSGVNSGLKFKNIRLEPEIRQEFFIEQP
ISIEATGDYHSFGSFVSGIAALPRIVTLHDFDISASSNNDKKTDIPQISYSVKAKTYRYVGATATTDVDVKDTKNDKKAD
VGGSK

Nucleotide


Download         Length: 738 bp        

>NTDB_id=391849 F9230_RS01295 WP_234951049.1 291996..292733(+) (pilO) [Acinetobacter johnsonii strain AJ14]
ATGAGTCGCGATGAATTTGAAGAATTAACATCAGAAAACCATGCTGTAACCAAGAAAAAAATGACGGTTGATAAGTTTTT
TCAACAGTTTAATACCTTAGATCCAAACAATTACGGTAGTTGGCCCTTTTCAGTCAAATTAACTTGTTGGATCTTTATCA
TTTTAGTAATTGGGGCATTGGGTTATTTTGTCGTGATTAAACCGAAGCTTGATGCGATTTCAAATGCACATGCACAAGAA
CAAAATTTACTGAATGAGTTTCGTGAAAAAGATTCAAAATTACGTAACTTGCAGCAATATCAAGCGCAATTGCAAGAAAT
GGAAGCTAATTTCAATCAGCAGTTAGAACAGTTGCCGAAAGAAACTGAAATTCCTGGTTTGGTTGAAGATATCAACGTGA
GCGGCGTAAACTCTGGATTAAAGTTTAAAAATATTCGTTTAGAACCAGAAATTCGCCAAGAGTTCTTTATCGAACAGCCG
ATTTCCATTGAAGCGACGGGTGATTATCATTCTTTTGGTTCATTTGTCAGTGGAATTGCAGCTTTACCGCGTATTGTGAC
CTTGCATGACTTCGATATCTCCGCATCAAGTAACAATGATAAAAAGACGGATATTCCGCAAATTTCATACAGTGTAAAAG
CGAAAACCTACCGTTATGTCGGGGCGACGGCTACAACGGATGTTGATGTAAAAGATACAAAAAATGATAAGAAAGCCGAT
GTGGGAGGTAGCAAGTAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(74-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

74.449

92.653

0.69
  comO Acinetobacter baylyi ADP1

72.398

90.204

0.653

Multiple sequence alignment