Detailed information NTG0390122

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: FSC09_RS07030
• Genome accession: NZ_CP044455
• Coordinates: 1455343..1456536 (+)
• Length: 397 a.a.
• NCBI ID: WP_045794791.1
• Uniprot ID: A0A6C0Y670
• Organism: Acinetobacter indicus strain B18
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1450343..1461536

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FSC09_RS07015 (FSC09_07020)	-	1450567..1451949 (-)	1383	WP_005178192.1	amino acid permease	-
FSC09_RS07020 (FSC09_07025)	hppD	1452101..1453159 (-)	1059	WP_016658083.1	4-hydroxyphenylpyruvate dioxygenase	-
FSC09_RS07025 (FSC09_07030)	-	1454034..1455089 (+)	1056	WP_127800221.1	3-deoxy-7-phosphoheptulonate synthase	-
FSC09_RS07030 (FSC09_07035)	pilU	1455343..1456536 (+)	1194	WP_045794791.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
FSC09_RS07035 (FSC09_07040)	-	1456595..1457776 (-)	1182	WP_127799913.1	MFS transporter	-
FSC09_RS07040 (FSC09_07045)	gcvH	1458032..1458406 (-)	375	WP_005178176.1	glycine cleavage system protein GcvH	-
FSC09_RS07045 (FSC09_07050)	-	1458475..1459503 (-)	1029	WP_005178175.1	helix-turn-helix transcriptional regulator	-
FSC09_RS07050 (FSC09_07055)	-	1459607..1460056 (-)	450	WP_016658087.1	MaoC family dehydratase	-

Sequence

Protein

• Length: 397 a.a.
• Molecular weight: 44672.05 Da
• Isoelectric Point: 6.2356

>NTDB_id=390122 FSC09_RS07030 WP_045794791.1 1455343..1456536(+) (pilU) [Acinetobacter indicus strain B18]
MYSAELLEDARKFMFHMLSKVVEFGGSDLFITADFPPSIKHQGMMKALGQQALTAEKTKLFAYSLMNEKQRSEFETDLEC
NFAISVPEVSRFRVNVFQQQLHVGMVIRTISAEIPNFSQLKLPESLKHIIMEKRGLVLVVGGTGSGKSTSLAAMIDHRNE
NSAGHIITVEDPVEYVHKHKKSMITHREVGVDSHSWHNALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
ANNANQTLDRIINFFPEERRNQLLMDLSSNMKAIISQRLVRTEDGKGRRAAVEIMLNTPLISELILKGQFHELKSIMGKS
RELGMQTFDQALFDLYNEGAISYEEAIRNADSMNELRLQIKLKSNRPSEASAAMSFSMLEEPKPEDEDTESAPQQSA

Nucleotide

• Length: 1194 bp

>NTDB_id=390122 FSC09_RS07030 WP_045794791.1 1455343..1456536(+) (pilU) [Acinetobacter indicus strain B18]
ATGTACAGTGCAGAATTACTCGAAGATGCGCGTAAATTTATGTTTCATATGCTCAGCAAAGTGGTTGAGTTTGGGGGATC
AGATTTATTTATTACCGCGGATTTTCCACCGAGCATTAAACATCAGGGCATGATGAAAGCGCTGGGACAACAGGCGCTGA
CTGCAGAAAAAACCAAGCTGTTTGCCTACAGCCTCATGAATGAAAAACAGCGTAGCGAGTTTGAAACTGATCTGGAATGC
AACTTTGCCATTAGTGTGCCGGAGGTATCGCGCTTTCGGGTCAATGTGTTTCAACAGCAGCTGCATGTCGGGATGGTAAT
TCGAACCATTAGCGCGGAAATTCCGAACTTTAGCCAGCTGAAACTGCCGGAATCGCTGAAGCATATTATTATGGAAAAGC
GCGGTCTGGTGCTGGTGGTGGGGGGAACCGGTTCAGGTAAATCGACCTCTTTGGCGGCGATGATTGATCATCGTAATGAA
AATTCAGCCGGGCATATTATTACCGTGGAAGACCCGGTGGAATATGTGCACAAGCATAAAAAGTCGATGATTACCCATCG
TGAAGTCGGGGTCGACAGCCATTCCTGGCACAATGCGCTGAAAAATACCTTACGTCAGGCACCGGATGTAATTTTAATTG
GCGAGATCCGCGACACCGAAACCATGGAACACGCAATTGCCTTTGCCGAAACCGGGCATTTATGTTTGGGCACTTTACAT
GCCAATAACGCCAACCAGACGCTGGACCGGATTATCAACTTTTTCCCGGAAGAACGCCGTAACCAGCTGCTGATGGATCT
GTCGTCCAATATGAAGGCCATTATTTCCCAGCGTCTGGTGCGTACCGAAGATGGCAAAGGGCGCCGTGCGGCAGTCGAGA
TTATGCTGAATACGCCGCTGATTTCTGAACTGATTTTAAAAGGCCAGTTCCATGAGTTAAAGTCGATTATGGGCAAATCG
CGTGAACTGGGCATGCAGACGTTTGATCAGGCTCTATTTGATTTGTATAACGAAGGCGCGATTTCCTATGAAGAAGCAAT
TCGTAATGCCGATTCGATGAATGAATTGCGCTTACAGATTAAATTGAAAAGTAACCGGCCAAGCGAAGCTTCGGCAGCTA
TGAGTTTTAGCATGCTGGAAGAGCCAAAACCGGAAGATGAAGATACGGAATCTGCTCCTCAACAGAGTGCTTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (117-282)

3D structure

• AlphaFold DB: A0A6C0Y670

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	57.568	93.199	0.537
pilU	Acinetobacter baylyi ADP1	54.42	91.184	0.496
pilU	Vibrio cholerae strain A1552	52.663	85.139	0.448
pilT	Acinetobacter baumannii strain A118	43.939	83.123	0.365
pilT	Acinetobacter nosocomialis M2	43.939	83.123	0.365
pilT	Acinetobacter baumannii D1279779	43.939	83.123	0.365
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.814	84.131	0.36