Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   F6Y24_RS18330 Genome accession   NZ_CP044334
Coordinates   4130713..4132446 (+) Length   577 a.a.
NCBI ID   WP_039512338.1    Uniprot ID   A0AAQ1AK58
Organism   Xanthomonas arboricola pv. pruni strain 15-088     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4125713..4137446
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F6Y24_RS18305 (F6Y24_18305) - 4126725..4126880 (-) 156 Protein_3475 SymE family type I addiction module toxin -
  F6Y24_RS18310 (F6Y24_18310) coaE 4126980..4127603 (-) 624 WP_043088081.1 dephospho-CoA kinase -
  F6Y24_RS18315 (F6Y24_18315) - 4127617..4128480 (-) 864 WP_150861917.1 A24 family peptidase -
  F6Y24_RS18320 (F6Y24_18320) pilC 4128487..4129746 (-) 1260 WP_043088082.1 type II secretion system F family protein Machinery gene
  F6Y24_RS18325 (F6Y24_18325) - 4130124..4130528 (+) 405 WP_043088084.1 pilin -
  F6Y24_RS18330 (F6Y24_18330) pilB 4130713..4132446 (+) 1734 WP_039512338.1 type IV-A pilus assembly ATPase PilB Machinery gene
  F6Y24_RS18335 (F6Y24_18335) pilR 4132682..4134076 (-) 1395 WP_051634076.1 sigma-54 dependent transcriptional regulator Regulator
  F6Y24_RS18340 (F6Y24_18340) - 4134269..4135882 (-) 1614 WP_039512337.1 HAMP domain-containing sensor histidine kinase -
  F6Y24_RS18345 (F6Y24_18345) sucC 4136114..4137283 (+) 1170 WP_016901264.1 ADP-forming succinate--CoA ligase subunit beta -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62578.76 Da        Isoelectric Point: 5.6006

>NTDB_id=389473 F6Y24_RS18330 WP_039512338.1 4130713..4132446(+) (pilB) [Xanthomonas arboricola pv. pruni strain 15-088]
MNVVIANLVGITGIARRLVQDGALDESAARLAMDQASAAKVPLPQWFAEKKLVTASQLAAANAVEFGMPLMDVSVFDANQ
NAVKLVSEELLQKYQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNASLGSSLGD
DDEGMGDLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAPV
KLSQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIEKLGYEADQQKL
FLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPD
IIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLPE
HALLAEGFTPAQIAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQSIGIRDLRQSALVK
AAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=389473 F6Y24_RS18330 WP_039512338.1 4130713..4132446(+) (pilB) [Xanthomonas arboricola pv. pruni strain 15-088]
ATGAACGTAGTCATTGCCAATCTGGTCGGCATTACCGGCATTGCTCGTCGGCTTGTCCAAGACGGGGCTTTAGATGAGTC
AGCGGCAAGGCTGGCGATGGATCAGGCTAGCGCTGCCAAGGTGCCTCTGCCCCAATGGTTTGCTGAGAAAAAATTGGTAA
CCGCGTCTCAACTTGCCGCCGCTAACGCGGTCGAATTCGGTATGCCACTGATGGACGTGTCGGTCTTCGACGCCAATCAG
AACGCGGTCAAACTGGTCAGTGAGGAGTTGCTCCAGAAGTATCAGGTACTGCCGCTGTTCAAGCGCGGCAACCGGTTGTT
CGTGGGGGTGAGCAACCCGACCCAGACCAGGGCGCTGGACGACATCAAGTTTCATACCAATTTGGTGGTCGAGCCGATCC
TGGTTGACGAGGACCAGATCCGTCGTACCTTGGAGCAGTGGCAGGCCAGCAACGCATCGTTGGGCTCGTCGCTCGGGGAC
GATGATGAGGGGATGGGCGACCTGGACGTGTCGGCCGGGGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGATGC
CAAGGGCGACGACACGCCGGTAGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCCGACATCC
ATTTCGAGCCCTATGAAGACGACTACCGGGTGCGGCTGCGGATCGATGGGCTGCTGAAGAACGTGGCCAAGGCGCCGGTG
AAGTTGAGCCAGCGTATCGCGGCACGCCTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGACGG
GCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTGAGTACCTTGCCGACATTGTTCGGCGAAAAGG
TGGTGTTGCGTATCCTGGACGGCAGCGCGGCCAAGCTGGGCATCGAGAAGCTGGGCTACGAGGCGGACCAGCAGAAGCTG
TTCCTGGAGGCGATCCACAAACCCTACGGCATGGTGCTGGTGACCGGGCCGACTGGTTCGGGCAAGACGGTGTCGCTGTA
TACGGCACTGGGTATCCTCAACGACGAGACGCGCAACATCTCCACCGCCGAGGACCCGGTAGAAATCCGCCTGCCTGGCG
TCAATCAGGTGCAGCAGAACAACAAGCGCGGCATGACTTTCGCCGCGGCGCTGCGCTCGTTCCTGCGCCAGGACCCGGAC
ATCATCATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATTGCGATCAAGGCCGCGCAGACCGGTCACATGGTGCT
GTCCACGCTGCATACCAACGATGCGCCGCAGACCATCGCGCGTCTGATGAACATGGGCATCGCGCCGTACAACATCACCT
CGTCGGTGACCCTGGTGATCGCGCAGCGTCTGGCGCGGCGCCTGTGCAACAACTGCAAGCGCAAATCGACCTTGCCCGAG
CATGCGTTGCTGGCCGAAGGCTTCACGCCTGCGCAGATCGCTGCCGGGATCGAGCTGTATGAGGCGGTCGGTTGCGACGA
GTGCACCGAAGGCTACAAGGGCCGTACCGGTATCTACCAGGTGATGCCGATGACCGACGAGATCGGCGCGATCGTGCTGG
AAGGCGGTAACGCGATGCAGATCGCCGAGGCGGCGCAGAGTATCGGCATCCGCGACCTGCGCCAGTCAGCGTTGGTCAAG
GCCGCGCACGGGGTGACCAGCCTGGCCGAGATCAATCGGGTGACAAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(200-467)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(48-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.458

98.44

0.546
  pilB Acinetobacter baylyi ADP1

55.282

98.44

0.544
  pilB Legionella pneumophila strain ERS1305867

52.548

98.614

0.518
  pilB Vibrio cholerae strain A1552

48.966

100

0.492
  pilF Neisseria gonorrhoeae MS11

48.768

98.44

0.48
  pilB Vibrio parahaemolyticus RIMD 2210633

46.964

97.054

0.456
  pilB Vibrio campbellii strain DS40M4

46.046

98.614

0.454
  pilB/pilB1 Synechocystis sp. PCC 6803

36.125

100

0.381
  pilF Thermus thermophilus HB27

40.192

90.121

0.362

Multiple sequence alignment