Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF/cglF   Type   Machinery gene
Locus tag   LPB220_RS01240 Genome accession   NZ_CP044230
Coordinates   212658..213110 (+) Length   150 a.a.
NCBI ID   WP_031572613.1    Uniprot ID   A0A5J6LK50
Organism   Streptococcus sp. LPB0220     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 207658..218110
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LPB220_RS01210 (LPB220_01210) - 209325..209714 (+) 390 WP_042759849.1 DUF1033 family protein -
  LPB220_RS01215 (LPB220_01215) comYA 209802..210743 (+) 942 WP_021154281.1 competence type IV pilus ATPase ComGA Machinery gene
  LPB220_RS01220 (LPB220_01220) comYB 210676..211707 (+) 1032 WP_176745544.1 competence type IV pilus assembly protein ComGB Machinery gene
  LPB220_RS01225 (LPB220_01225) comYC 211704..212021 (+) 318 WP_150905144.1 competence type IV pilus major pilin ComGC Machinery gene
  LPB220_RS01230 (LPB220_01230) comYD 212011..212415 (+) 405 WP_003004513.1 competence type IV pilus minor pilin ComGD Machinery gene
  LPB220_RS01235 (LPB220_01235) comGE 212381..212668 (+) 288 WP_003004353.1 competence type IV pilus minor pilin ComGE -
  LPB220_RS01240 (LPB220_01240) comGF/cglF 212658..213110 (+) 453 WP_031572613.1 competence type IV pilus minor pilin ComGF Machinery gene
  LPB220_RS01245 (LPB220_01245) comGG 213067..213534 (+) 468 WP_225305918.1 competence type IV pilus minor pilin ComGG -
  LPB220_RS01250 (LPB220_01250) comYH 213565..214518 (+) 954 WP_021154287.1 class I SAM-dependent methyltransferase Machinery gene
  LPB220_RS01255 (LPB220_01255) - 214570..215763 (+) 1194 WP_150905146.1 acetate kinase -
  LPB220_RS01260 (LPB220_01260) - 215836..216567 (+) 732 WP_150905148.1 CPBP family intramembrane glutamic endopeptidase -
  LPB220_RS01265 (LPB220_01265) folP 216730..217680 (+) 951 WP_070586317.1 dihydropteroate synthase -

Sequence

Protein


Download         Length: 150 a.a.        Molecular weight: 17268.65 Da        Isoelectric Point: 5.9195

>NTDB_id=388962 LPB220_RS01240 WP_031572613.1 212658..213110(+) (comGF/cglF) [Streptococcus sp. LPB0220]
MWNNGKVKAFTLLEALVALLVLSGGVLVFQGLTKLLHAELDYQAHQKQEEWVLFQQQLQVELDRSHFEKVADNHIYLVQD
QKPIAIGQSKGDDIRKTDDKGRGYQPMISGVRSSQIWQEGELLHLRLNFEEGLEREYVYHVVPAIQNEKS

Nucleotide


Download         Length: 453 bp        

>NTDB_id=388962 LPB220_RS01240 WP_031572613.1 212658..213110(+) (comGF/cglF) [Streptococcus sp. LPB0220]
ATGTGGAATAACGGAAAGGTCAAGGCCTTTACCCTGCTCGAAGCCTTGGTCGCTCTCTTGGTGCTCAGTGGGGGTGTGTT
GGTCTTTCAAGGCTTGACCAAGCTCCTGCATGCTGAATTGGACTACCAGGCGCATCAAAAGCAGGAAGAATGGGTACTTT
TTCAGCAACAATTGCAGGTGGAATTGGACCGGAGTCATTTTGAAAAAGTAGCGGACAATCATATCTACTTGGTTCAGGAT
CAAAAACCAATTGCTATTGGCCAATCTAAAGGAGATGATATCCGAAAAACAGATGATAAGGGCAGAGGCTATCAGCCTAT
GATTTCAGGGGTTCGCTCTAGCCAAATTTGGCAGGAGGGAGAGCTCCTTCATCTGCGGTTGAATTTTGAAGAAGGTCTAG
AAAGGGAGTATGTCTACCATGTGGTACCAGCGATACAAAATGAAAAAAGTTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(43-134)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5J6LK50

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF/cglF Streptococcus mitis NCTC 12261

55.396

92.667

0.513
  comGF/cglF Streptococcus mitis SK321

54.676

92.667

0.507
  comGF/cglF Streptococcus pneumoniae Rx1

55.882

90.667

0.507
  comGF/cglF Streptococcus pneumoniae D39

55.882

90.667

0.507
  comGF/cglF Streptococcus pneumoniae R6

55.882

90.667

0.507
  comGF/cglF Streptococcus pneumoniae TIGR4

55.882

90.667

0.507
  comYF Streptococcus mutans UA159

42.222

90

0.38
  comYF Streptococcus mutans UA140

42.222

90

0.38

Multiple sequence alignment