Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   F5I99_RS10005 Genome accession   NZ_CP044222
Coordinates   2172906..2174624 (+) Length   572 a.a.
NCBI ID   WP_225307369.1    Uniprot ID   -
Organism   Nitrincola iocasae strain KXZD1103     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2167906..2179624
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F5I99_RS09990 (F5I99_09990) - 2168910..2169728 (+) 819 WP_151059086.1 pyruvate, water dikinase regulatory protein -
  F5I99_RS09995 (F5I99_09995) pilR 2169738..2171141 (-) 1404 WP_151055606.1 sigma-54 dependent transcriptional regulator Regulator
  F5I99_RS10000 (F5I99_10000) - 2171141..2172742 (-) 1602 WP_151055608.1 nitrogen regulation protein NR(II) -
  F5I99_RS10005 (F5I99_10005) pilB 2172906..2174624 (+) 1719 WP_225307369.1 type IV-A pilus assembly ATPase PilB Machinery gene
  F5I99_RS10010 (F5I99_10010) pilC 2174642..2175874 (+) 1233 WP_151055610.1 type II secretion system F family protein Machinery gene
  F5I99_RS10015 (F5I99_10015) pilD 2175890..2176744 (+) 855 WP_151055611.1 A24 family peptidase Machinery gene
  F5I99_RS10020 (F5I99_10020) - 2176776..2177858 (-) 1083 WP_151055613.1 ABC transporter ATP-binding protein -
  F5I99_RS10025 (F5I99_10025) - 2177855..2179471 (-) 1617 WP_225307370.1 iron ABC transporter permease -

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 63091.38 Da        Isoelectric Point: 5.2409

>NTDB_id=388891 F5I99_RS10005 WP_225307369.1 2172906..2174624(+) (pilB) [Nitrincola iocasae strain KXZD1103]
MAQGAQPLSGLARRLVMDGVFSSEVATDAINKSRDAKQPFVSYIIKQRLVSAHRAASAAAEEFGLPLLDLDAIPGDSLPK
EIVSDALITKHHILPLMKRDNRLFVAVADPTNMQGIDEIKFQTGIITEVVIVEEDKLSAALDVYLDQQQSSALGDLDDAD
LDNLDIDDSSQKKDEEQSAEDAASEAPIVRFINKVLMDAIKTGASDIHFEPYEKSYRVRCRIDGILQEVHRPPMNLATRL
AARLKVMSQMDISEKRVPQDGRIKMKISKTRAIDFRVNSLPTLWGEKIVLRILDPTSAQIGVEALGFEPDQKKIYLTTLE
QPQGMILVTGPTGSGKTVTLYTGLNILNTAERNISTAEDPVEINMDGINQVHVNIKVGLTFAEALRSFLRQDPDVVMVGE
IRDLETAEIAIKAAQTGHMVLSTLHTNSAPETLTRLRNMGVPAFNVATSVSLIIAQRLGRRLCESCKRPTDLPEATLLEE
GFKPDQIPDLHLYEANHEGCSKCNRGYKGRVGIYEMLAMTQEVAECIMENGNSLDILRVARKQGFKTLRESGLLKVANGL
TSLEEMNRVIKT

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=388891 F5I99_RS10005 WP_225307369.1 2172906..2174624(+) (pilB) [Nitrincola iocasae strain KXZD1103]
GTGGCACAAGGGGCTCAACCACTTAGCGGACTTGCCAGACGCCTTGTCATGGATGGTGTTTTTTCATCCGAAGTTGCAAC
AGATGCAATAAACAAGTCACGCGATGCCAAGCAACCATTTGTCAGCTATATCATTAAACAACGCCTGGTCAGTGCACACC
GCGCCGCTTCAGCCGCTGCTGAAGAGTTTGGTCTGCCGTTGCTGGATCTGGATGCCATCCCCGGAGATTCACTCCCCAAA
GAGATTGTTTCTGACGCTCTGATCACCAAGCACCACATTTTGCCCTTAATGAAACGTGACAATCGTTTATTTGTTGCGGT
GGCCGACCCAACCAATATGCAGGGCATTGATGAAATCAAATTCCAGACCGGCATCATTACTGAAGTTGTCATTGTAGAAG
AAGATAAACTGTCAGCCGCACTGGATGTCTACCTCGATCAGCAACAAAGTTCTGCACTGGGTGACCTTGATGACGCTGAT
CTGGACAACCTTGATATTGACGATAGCTCTCAGAAAAAAGACGAAGAGCAGTCGGCTGAAGATGCAGCCAGTGAAGCGCC
AATCGTGCGTTTTATCAATAAAGTGCTTATGGATGCGATCAAGACCGGCGCATCAGATATTCACTTTGAACCTTACGAAA
AAAGCTACCGGGTACGCTGTCGTATCGATGGTATATTGCAAGAGGTGCATCGCCCACCGATGAATCTGGCTACTCGTCTG
GCAGCCCGACTCAAAGTCATGTCACAAATGGATATTTCCGAAAAGCGCGTGCCACAAGACGGGCGCATCAAGATGAAAAT
ATCCAAGACTCGCGCCATTGATTTTCGTGTTAACTCGTTACCGACTCTCTGGGGCGAGAAAATTGTATTACGTATACTCG
ATCCGACCAGTGCCCAAATAGGTGTTGAAGCACTGGGCTTTGAGCCCGATCAGAAAAAAATCTACCTGACTACCCTGGAA
CAACCACAGGGCATGATTCTGGTTACAGGACCAACTGGTTCTGGTAAAACCGTTACCCTCTATACCGGCTTGAACATTCT
CAATACCGCTGAACGCAATATCTCAACTGCTGAAGACCCGGTCGAGATCAACATGGACGGCATCAACCAGGTACACGTGA
ATATCAAAGTGGGCCTGACCTTTGCTGAAGCCTTGCGCTCTTTCCTGCGACAGGACCCGGATGTCGTCATGGTTGGTGAG
ATACGCGATCTGGAAACAGCTGAAATTGCCATTAAAGCAGCACAAACTGGCCACATGGTTCTATCAACGCTACACACCAA
CAGCGCACCCGAGACACTGACGCGTCTGCGCAACATGGGTGTCCCGGCCTTTAACGTAGCCACCTCAGTCAGCCTGATAA
TAGCCCAGCGATTAGGACGGCGTTTATGTGAATCCTGTAAACGCCCGACTGACCTGCCGGAGGCCACACTGCTTGAGGAA
GGGTTTAAACCCGATCAGATCCCTGACCTGCACCTTTACGAAGCAAATCATGAGGGTTGCTCAAAATGTAACCGTGGTTA
CAAGGGACGTGTCGGTATTTATGAGATGCTTGCCATGACACAAGAGGTTGCCGAATGTATTATGGAAAACGGCAATTCAC
TGGATATATTACGCGTTGCACGCAAACAGGGATTCAAAACTCTCCGTGAATCCGGACTACTTAAAGTCGCCAATGGCCTG
ACTAGTCTTGAAGAAATGAATCGCGTTATTAAAACCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-149)

Type II/IV secretion system protein (T2SSE)

(194-461)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.511

98.601

0.577
  pilB Acinetobacter baylyi ADP1

57.447

98.601

0.566
  pilB Legionella pneumophila strain ERS1305867

54.497

99.126

0.54
  pilB Vibrio cholerae strain A1552

55.039

90.21

0.497
  pilF Neisseria gonorrhoeae MS11

49.558

98.776

0.49
  pilB Vibrio parahaemolyticus RIMD 2210633

52.148

89.51

0.467
  pilB Vibrio campbellii strain DS40M4

49.812

93.007

0.463

Multiple sequence alignment