Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   F5I99_RS10015 Genome accession   NZ_CP044222
Coordinates   2175890..2176744 (+) Length   284 a.a.
NCBI ID   WP_151055611.1    Uniprot ID   -
Organism   Nitrincola iocasae strain KXZD1103     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2170890..2181744
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F5I99_RS10000 (F5I99_10000) - 2171141..2172742 (-) 1602 WP_151055608.1 nitrogen regulation protein NR(II) -
  F5I99_RS10005 (F5I99_10005) pilB 2172906..2174624 (+) 1719 WP_225307369.1 type IV-A pilus assembly ATPase PilB Machinery gene
  F5I99_RS10010 (F5I99_10010) pilC 2174642..2175874 (+) 1233 WP_151055610.1 type II secretion system F family protein Machinery gene
  F5I99_RS10015 (F5I99_10015) pilD 2175890..2176744 (+) 855 WP_151055611.1 A24 family peptidase Machinery gene
  F5I99_RS10020 (F5I99_10020) - 2176776..2177858 (-) 1083 WP_151055613.1 ABC transporter ATP-binding protein -
  F5I99_RS10025 (F5I99_10025) - 2177855..2179471 (-) 1617 WP_225307370.1 iron ABC transporter permease -
  F5I99_RS10030 (F5I99_10030) - 2179578..2180576 (-) 999 WP_151055615.1 iron ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 284 a.a.        Molecular weight: 31424.48 Da        Isoelectric Point: 7.6405

>NTDB_id=388893 F5I99_RS10015 WP_151055611.1 2175890..2176744(+) (pilD) [Nitrincola iocasae strain KXZD1103]
MLMFELFAAHTWWAVFFALLLALLVGSFLNVVILRLPVMMEREWQAAIAADSTTTKPQDNFNLAVPRSRCNHCGHLIRWY
ENIPVISWLALRGKCSSCSSVISPRYPLIELLSGLLCAFIAWQYGITLTGLALMLLTWALIALTFIDIDHQLLPDSITLP
LLWLGLLLNSFEVFTTLGNAVWGAALGYLALWCVYWAFKLLTGKEGMGYGDFKLLAALGAWGGAISLPMIILFSSVAGVL
LAGGMIALRKHQAANPLPFGPYLAIAGWFAILWGEPIMRWYLSS

Nucleotide


Download         Length: 855 bp        

>NTDB_id=388893 F5I99_RS10015 WP_151055611.1 2175890..2176744(+) (pilD) [Nitrincola iocasae strain KXZD1103]
ATGTTAATGTTTGAACTCTTTGCGGCCCACACCTGGTGGGCCGTTTTCTTCGCTCTGCTGCTGGCTTTGTTGGTAGGCAG
TTTTCTGAATGTAGTTATTTTGCGTTTACCGGTAATGATGGAGCGTGAATGGCAAGCGGCCATTGCCGCAGACTCAACTA
CGACGAAACCGCAAGACAACTTCAACCTGGCAGTTCCACGCTCGCGTTGCAACCACTGTGGGCATTTGATTCGCTGGTAT
GAAAATATTCCTGTGATCAGTTGGCTGGCCTTACGGGGCAAGTGCAGCTCATGCAGCAGCGTGATCAGCCCTCGCTATCC
GTTGATTGAGCTGCTCAGTGGTCTTCTATGTGCATTCATTGCCTGGCAGTACGGTATAACGCTGACAGGACTGGCGCTGA
TGTTGCTGACCTGGGCATTGATTGCCTTGACGTTTATCGATATAGACCACCAACTCCTGCCCGACAGTATTACCCTGCCG
CTGCTCTGGCTGGGATTACTACTTAACAGCTTTGAAGTATTTACTACACTTGGCAACGCCGTCTGGGGTGCTGCATTAGG
CTACCTGGCGCTCTGGTGTGTCTATTGGGCGTTTAAACTGTTGACCGGCAAGGAAGGCATGGGTTACGGCGACTTTAAGC
TCCTGGCCGCACTGGGCGCCTGGGGTGGGGCCATCAGCTTACCCATGATTATTTTGTTCTCATCGGTAGCTGGCGTTCTA
CTGGCGGGTGGCATGATCGCACTGCGTAAGCATCAGGCCGCAAACCCTTTGCCTTTTGGCCCTTATCTGGCCATTGCCGG
CTGGTTTGCAATACTCTGGGGCGAGCCAATCATGCGCTGGTACCTAAGTAGTTAA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(134-242)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(20-124)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

53.846

100

0.542
  pilD Vibrio campbellii strain DS40M4

53.521

100

0.535
  pilD Acinetobacter baumannii D1279779

53.008

93.662

0.496
  pilD Acinetobacter nosocomialis M2

52.632

93.662

0.493
  pilD Neisseria gonorrhoeae MS11

50.185

95.423

0.479

Multiple sequence alignment