Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   F6X00_RS06745 Genome accession   NZ_CP044206
Coordinates   1422964..1424652 (-) Length   562 a.a.
NCBI ID   WP_182290674.1    Uniprot ID   -
Organism   Vibrio vulnificus strain Vv180806     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1417964..1429652
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F6X00_RS06715 (F6X00_06840) rplS 1418516..1418869 (+) 354 WP_011079544.1 50S ribosomal protein L19 -
  F6X00_RS06720 (F6X00_06845) yacG 1419086..1419280 (-) 195 WP_026060801.1 DNA gyrase inhibitor YacG -
  F6X00_RS06725 (F6X00_06850) zapD 1419343..1420083 (-) 741 WP_011079546.1 cell division protein ZapD -
  F6X00_RS06730 (F6X00_06855) coaE 1420116..1420724 (-) 609 WP_039554617.1 dephospho-CoA kinase -
  F6X00_RS06735 (F6X00_06860) pilD 1420726..1421595 (-) 870 WP_043877313.1 A24 family peptidase Machinery gene
  F6X00_RS06740 (F6X00_06865) pilC 1421689..1422915 (-) 1227 WP_182290672.1 type II secretion system F family protein Machinery gene
  F6X00_RS06745 (F6X00_06870) pilB 1422964..1424652 (-) 1689 WP_182290674.1 type IV-A pilus assembly ATPase PilB Machinery gene
  F6X00_RS06750 (F6X00_06875) - 1424662..1425093 (-) 432 WP_130341685.1 pilin -
  F6X00_RS06755 (F6X00_06880) nadC 1425313..1426200 (-) 888 WP_182290675.1 carboxylating nicotinate-nucleotide diphosphorylase -
  F6X00_RS06760 (F6X00_06885) ampD 1426293..1426865 (+) 573 WP_108695085.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  F6X00_RS06765 (F6X00_06890) pdhR 1427254..1428021 (+) 768 WP_011079554.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62386.38 Da        Isoelectric Point: 5.5312

>NTDB_id=388758 F6X00_RS06745 WP_182290674.1 1422964..1424652(-) (pilB) [Vibrio vulnificus strain Vv180806]
MISNLATILRQANQLSLTQEQACRETIQASGVTAPEALLQLGFFQPDELTEQLSAIFGLPRTDLNQYDYQDLCNQLGLRE
LITRHQALPLQKSNAGLILAVADPTNIQAEDDFRFATGVQVEIVLADCQELQAAIRRLYGKSIKQSQGSGLKEIHQDELA
NLVEVSDDEVESIEDLSQDESPVSRFINQILLDAVRKGASDIHFEPYENQYRVRLRCDGILVEVQQPPSHLSRRLSARIK
ILSKLDIAERRLPQDGRIKLRLNQDTAIDMRVSTLPTLFGEKIVLRLLDSSSSSLDIDKLGYNPQQKQLYLDALRKPQGM
ILMTGPTGSGKTVSLYTGLSLLNRPEVNISTAEDPVEINLPGINQVQVKPKIGFGFAEALRSFLRQDPDIVMVGEIRDLE
TAEIAVKAAQTGHLVLSTLHTNSAAETIVRLSNMGIASFNLASSLSLIIAQRLARRLCSHCKQPHPYNATFELLGLKADD
LIYQADRQGCHHCTHGYSGRVGIYEVMPFDEQLAEAIVKGASVQSLEMLARQKGMMTLKDSGLEKLKQGITSLEELQRVL
YL

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=388758 F6X00_RS06745 WP_182290674.1 1422964..1424652(-) (pilB) [Vibrio vulnificus strain Vv180806]
ATGATATCCAATCTTGCCACTATTCTTCGTCAGGCCAACCAACTTAGCCTAACGCAAGAACAAGCGTGTCGAGAGACAAT
ACAAGCTTCCGGTGTCACAGCACCGGAAGCTTTGTTGCAACTGGGATTTTTCCAGCCGGATGAATTAACGGAGCAGCTCA
GCGCGATTTTTGGCTTACCACGCACTGACCTCAACCAATATGACTACCAAGATCTGTGTAATCAACTTGGGTTACGTGAG
CTGATCACTCGTCACCAAGCACTGCCACTGCAAAAAAGCAACGCTGGTTTAATACTCGCGGTTGCTGACCCTACCAATAT
TCAAGCTGAAGATGATTTCCGTTTTGCCACAGGTGTGCAGGTGGAGATTGTTTTGGCCGATTGCCAAGAGCTGCAAGCGG
CGATTCGTAGACTCTATGGAAAGAGCATCAAACAGAGCCAAGGCTCTGGTTTAAAAGAGATTCACCAAGATGAGCTGGCG
AATTTAGTCGAGGTTTCAGATGACGAAGTCGAGTCCATTGAAGACTTAAGCCAAGATGAATCTCCCGTTAGCCGCTTTAT
CAACCAAATTCTGCTGGATGCGGTACGCAAAGGCGCATCTGATATTCATTTTGAACCGTACGAAAACCAATACCGCGTGC
GATTGCGTTGCGATGGTATTTTGGTGGAAGTGCAGCAGCCTCCAAGCCATTTAAGCCGCCGTTTATCCGCGAGGATCAAG
ATCCTCTCCAAGCTGGATATTGCGGAAAGGCGTCTGCCACAAGATGGACGGATTAAGCTGCGCCTCAACCAAGACACCGC
GATTGATATGCGTGTTTCCACCTTACCCACACTGTTTGGTGAAAAAATTGTGTTGCGACTTCTCGACAGCTCGAGCAGTA
GCTTGGATATTGATAAGTTAGGCTACAACCCGCAACAAAAGCAGCTCTACCTCGATGCCCTACGCAAACCGCAAGGGATG
ATTTTGATGACAGGCCCAACAGGAAGCGGTAAAACCGTTTCGCTGTACACAGGCTTGAGTTTACTTAACCGGCCAGAAGT
GAATATCTCCACCGCGGAAGACCCAGTCGAGATTAACCTCCCGGGAATCAACCAAGTGCAAGTGAAACCCAAAATTGGTT
TTGGTTTTGCAGAAGCATTGCGTTCTTTTCTCCGTCAAGACCCAGATATCGTCATGGTGGGGGAAATTCGAGATTTGGAA
ACCGCCGAGATCGCCGTCAAAGCAGCCCAAACCGGCCACCTTGTACTATCCACCTTACATACCAACTCAGCTGCTGAAAC
CATTGTTCGTCTATCTAACATGGGGATCGCCAGCTTTAACTTGGCTTCATCCTTGAGTTTGATCATTGCTCAGCGTCTGG
CTCGTCGGTTATGCAGCCATTGCAAGCAACCTCATCCATACAACGCAACATTTGAGTTACTCGGCCTAAAAGCTGACGAC
TTGATTTATCAAGCTGATAGACAGGGTTGCCATCATTGTACTCATGGTTATTCCGGTCGTGTCGGTATATACGAAGTCAT
GCCTTTTGATGAACAGCTTGCAGAGGCGATCGTTAAAGGCGCCTCGGTACAAAGCTTAGAGATGCTTGCTCGACAAAAAG
GCATGATGACACTCAAAGATTCCGGCCTTGAAAAACTCAAACAGGGCATCACCAGCTTAGAAGAGCTGCAACGCGTCCTC
TATTTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(189-456)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

76.69

100

0.767
  pilB Vibrio parahaemolyticus RIMD 2210633

76.157

100

0.762
  pilB Vibrio cholerae strain A1552

74.6

100

0.747
  pilB Legionella pneumophila strain ERS1305867

53.216

91.281

0.486
  pilB Acinetobacter baumannii D1279779

51.538

92.527

0.477
  pilB Acinetobacter baylyi ADP1

50.478

93.06

0.47
  pilF Neisseria gonorrhoeae MS11

45.31

100

0.456
  pilF Thermus thermophilus HB27

41.039

99.288

0.407
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.491

94.306

0.363

Multiple sequence alignment