Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   F6R98_RS18425 Genome accession   NZ_CP044205
Coordinates   4040843..4042528 (+) Length   561 a.a.
NCBI ID   WP_153250317.1    Uniprot ID   -
Organism   Candidatus Methylospira mobilis strain Shm1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4035843..4047528
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F6R98_RS18400 (F6R98_18325) - 4037076..4038872 (+) 1797 WP_153250313.1 NAD(P)H-dependent oxidoreductase subunit E -
  F6R98_RS18405 (F6R98_18330) - 4038884..4039606 (+) 723 WP_153251138.1 2Fe-2S iron-sulfur cluster-binding protein -
  F6R98_RS18410 (F6R98_18335) - 4039591..4040133 (+) 543 WP_153250314.1 NADP oxidoreductase -
  F6R98_RS18415 (F6R98_18340) moaD 4040144..4040377 (+) 234 WP_153250315.1 molybdopterin converting factor subunit 1 -
  F6R98_RS18420 (F6R98_18345) - 4040379..4040819 (+) 441 WP_153250316.1 molybdenum cofactor biosynthesis protein MoaE -
  F6R98_RS18425 (F6R98_18350) pilB 4040843..4042528 (+) 1686 WP_153250317.1 type IV-A pilus assembly ATPase PilB Machinery gene
  F6R98_RS18430 (F6R98_18355) pilC 4043071..4044282 (+) 1212 WP_153250318.1 type II secretion system F family protein Machinery gene
  F6R98_RS18435 (F6R98_18360) coaE 4044286..4044888 (+) 603 WP_153250319.1 dephospho-CoA kinase -
  F6R98_RS18440 (F6R98_18365) rlmM 4045015..4046112 (-) 1098 WP_153250320.1 23S rRNA (cytidine(2498)-2'-O)-methyltransferase RlmM -
  F6R98_RS18445 (F6R98_18370) rplS 4046474..4046824 (-) 351 WP_153250321.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 61107.40 Da        Isoelectric Point: 6.6854

>NTDB_id=388726 F6R98_RS18425 WP_153250317.1 4040843..4042528(+) (pilB) [Candidatus Methylospira mobilis strain Shm1]
MDASPPASGLINRLLQEGLLDADKLHDCLQSARKQALPPVTVLVQQQVISGAVLAHFVAQEFGLPLFDLNALNHSALPQH
AIKPELIRSQQLLPLFRRGNRLDIATADPSNLQGLNDIKFQTGLSLNCIVVEAHKLAKLIETLYGNAARDLQELIDGSHG
DTSAATQDAGLGRIDEEPIVRFVNGLLSDAVRKEASDIHIEPYEHSFRIRFRHDGILHEVAAPPATLAARIVSRIKVMAH
LNIAEHRLPQDGRIKTVSPEGITINFRVNTCPTLYGEKIVLRLLDSDHARLDIDQLGFEPEQRQAFLDALKKPYGMILVT
GPTGSGKTVTLYSGLGKLNTADVNISTAEDPVEIVLPGVNQVNVTPKTGLTFAEAMRAFLRQDPDILMVGEIRDLETAEI
AVKAAQTGHLLLSTLHTNDAPQTLTRLLQMGIPPFNIASSVLLIIAQRLARRLCPHCKRAEKLPLASLLEAGFSEQEIAG
LTVYAPTGCEHCVKGYRGRVGIYQVMPISDALNRIILEGGNVIALNKQAQHEGIVSLREAGLKKVADGITSLAEIDRITR
D

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=388726 F6R98_RS18425 WP_153250317.1 4040843..4042528(+) (pilB) [Candidatus Methylospira mobilis strain Shm1]
TTGGATGCCTCACCTCCTGCAAGCGGACTGATCAACCGGCTACTGCAGGAAGGCCTGCTCGACGCCGACAAACTGCACGA
CTGCCTGCAATCGGCCCGGAAGCAAGCATTGCCCCCGGTCACCGTTCTGGTACAGCAACAGGTAATCTCCGGGGCCGTTC
TGGCGCATTTTGTCGCCCAAGAGTTCGGGCTGCCGTTATTCGATCTGAACGCGCTCAATCATAGCGCGCTGCCGCAGCAC
GCCATCAAACCCGAGCTGATACGCAGCCAGCAACTGTTGCCGCTGTTCCGGCGCGGAAACCGCCTCGACATCGCCACGGC
GGACCCGAGCAATTTACAAGGCCTGAACGACATAAAATTTCAGACCGGTCTGAGTTTAAACTGCATCGTCGTCGAAGCCC
ACAAGCTGGCGAAACTGATAGAAACGCTGTACGGCAATGCCGCTCGCGACCTGCAGGAGCTGATCGACGGCAGCCACGGC
GATACTTCAGCCGCCACGCAGGACGCGGGCCTCGGTCGGATCGATGAAGAACCCATCGTACGCTTCGTCAACGGCCTGCT
CTCCGATGCGGTCAGGAAGGAAGCTTCCGACATTCATATCGAACCCTACGAACACAGCTTCCGCATCCGTTTCCGCCACG
ACGGCATCCTGCACGAAGTCGCCGCGCCGCCGGCAACGCTAGCAGCACGTATCGTGTCGCGCATCAAGGTCATGGCGCAT
CTGAACATCGCCGAGCATCGCCTCCCTCAGGACGGGCGCATAAAAACCGTGTCGCCGGAAGGCATAACCATCAATTTCCG
CGTCAACACCTGCCCGACGCTGTATGGCGAAAAGATCGTGCTCAGGCTGCTGGACTCGGATCACGCCCGCCTCGACATCG
ATCAGCTCGGCTTCGAGCCCGAGCAAAGACAGGCTTTTCTGGATGCGCTTAAAAAACCCTACGGCATGATACTGGTCACC
GGTCCGACCGGCAGCGGAAAAACCGTCACCCTGTATTCAGGTTTAGGCAAGCTGAATACCGCCGATGTGAATATTTCAAC
GGCGGAAGATCCGGTTGAAATCGTACTGCCTGGAGTCAATCAGGTTAACGTCACCCCGAAAACAGGGCTCACCTTCGCCG
AAGCCATGCGCGCCTTTCTGCGCCAGGACCCCGATATTTTGATGGTAGGCGAAATTCGCGACCTCGAAACCGCTGAAATT
GCGGTGAAAGCTGCACAAACCGGCCATTTGCTGCTTTCCACGCTGCACACCAACGATGCGCCGCAAACTTTGACGCGCTT
GTTGCAGATGGGCATACCGCCGTTCAACATTGCTTCGTCGGTATTGCTGATCATCGCGCAACGGCTGGCGCGCCGCTTGT
GTCCGCACTGCAAAAGGGCCGAAAAGCTGCCCCTGGCCTCGCTGCTGGAAGCCGGTTTCAGTGAACAGGAAATAGCCGGA
CTGACCGTCTATGCGCCTACCGGATGCGAGCATTGTGTCAAGGGTTACCGTGGCCGCGTGGGTATTTATCAGGTCATGCC
GATTTCGGACGCGCTCAACCGCATCATACTGGAAGGCGGCAATGTCATCGCGCTGAACAAACAGGCCCAGCATGAAGGCA
TAGTCAGCCTGCGAGAAGCGGGTCTGAAAAAAGTGGCGGACGGTATTACCAGTCTGGCGGAAATAGATCGGATAACGAGG
GATTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-148)

Type II/IV secretion system protein (T2SSE)

(186-452)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

50.977

100

0.512
  pilB Acinetobacter baylyi ADP1

50.977

100

0.512
  pilB Acinetobacter baumannii D1279779

49.215

100

0.503
  pilB Vibrio cholerae strain A1552

50.758

94.118

0.478
  pilF Neisseria gonorrhoeae MS11

46.631

100

0.469
  pilB Vibrio parahaemolyticus RIMD 2210633

46.333

99.643

0.462
  pilB Vibrio campbellii strain DS40M4

46.835

98.574

0.462
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.176

100

0.403
  pilF Thermus thermophilus HB27

40.429

99.643

0.403
  pilB/pilB1 Synechocystis sp. PCC 6803

35.772

100

0.392

Multiple sequence alignment