Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   FOB52_RS10470 Genome accession   NZ_CP044077
Coordinates   2232756..2233190 (-) Length   144 a.a.
NCBI ID   WP_150332210.1    Uniprot ID   -
Organism   Pasteurella multocida strain FDAARGOS_644     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2227756..2238190
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FOB52_RS10440 (FOB52_10440) - 2228338..2228586 (-) 249 WP_115299813.1 GNAT family N-acetyltransferase -
  FOB52_RS10445 (FOB52_10445) yacG 2228586..2228789 (-) 204 WP_005719879.1 DNA gyrase inhibitor YacG -
  FOB52_RS10450 (FOB52_10450) coaE 2228800..2229420 (-) 621 WP_115299814.1 dephospho-CoA kinase -
  FOB52_RS10455 (FOB52_10455) - 2229432..2230157 (-) 726 WP_115299815.1 A24 family peptidase -
  FOB52_RS10460 (FOB52_10460) pilC 2230157..2231368 (-) 1212 WP_115299816.1 type II secretion system F family protein Machinery gene
  FOB52_RS10465 (FOB52_10465) pilB 2231368..2232756 (-) 1389 WP_150332209.1 GspE/PulE family protein Machinery gene
  FOB52_RS10470 (FOB52_10470) pilA 2232756..2233190 (-) 435 WP_150332210.1 prepilin peptidase-dependent pilin Machinery gene
  FOB52_RS10475 (FOB52_10475) ampD 2233350..2233916 (+) 567 WP_005719130.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FOB52_RS10480 (FOB52_10480) rppH 2234502..2235098 (+) 597 WP_083004799.1 RNA pyrophosphohydrolase -
  FOB52_RS10485 (FOB52_10485) - 2235101..2235895 (+) 795 WP_083004796.1 sulfite exporter TauE/SafE family protein -
  FOB52_RS10490 (FOB52_10490) lgt 2235905..2236708 (+) 804 WP_005751073.1 prolipoprotein diacylglyceryl transferase -
  FOB52_RS10495 (FOB52_10495) - 2236714..2237565 (+) 852 WP_115097855.1 thymidylate synthase -
  FOB52_RS10500 (FOB52_10500) tadA 2237592..2238080 (+) 489 WP_083004788.1 tRNA adenosine(34) deaminase TadA -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15028.45 Da        Isoelectric Point: 9.1281

>NTDB_id=387522 FOB52_RS10470 WP_150332210.1 2232756..2233190(-) (pilA) [Pasteurella multocida strain FDAARGOS_644]
MKKAIFFSIKKGFTLIELMIVIAIIAILATIAVPSYQNYTKKAAISELLQAAAPYRAEVELCIYNTNSTTNCNAGSHGIR
ADAAASKKYLKAITVKAGVITVTGQGGLEGISYTLTASGNAAQGVSWTVNCGAQSDIFPAGFCA

Nucleotide


Download         Length: 435 bp        

>NTDB_id=387522 FOB52_RS10470 WP_150332210.1 2232756..2233190(-) (pilA) [Pasteurella multocida strain FDAARGOS_644]
ATGAAAAAAGCCATTTTCTTTTCGATTAAAAAAGGGTTTACGCTAATTGAATTAATGATTGTCATTGCCATTATTGCGAT
TTTAGCCACGATTGCCGTGCCATCTTATCAAAATTATACAAAAAAAGCGGCAATCTCTGAATTATTGCAAGCCGCTGCTC
CTTATCGTGCCGAGGTAGAACTTTGTATCTATAATACAAATAGTACAACGAATTGTAATGCAGGAAGTCATGGTATTCGT
GCTGATGCGGCAGCAAGTAAAAAATATTTAAAAGCCATCACGGTTAAAGCTGGGGTAATTACTGTCACTGGACAAGGGGG
CTTGGAAGGAATTAGCTATACGCTGACAGCGAGTGGTAACGCAGCTCAAGGTGTTTCTTGGACAGTGAATTGTGGTGCTC
AATCTGACATTTTCCCAGCAGGATTTTGCGCATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(42-141)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

63.194

100

0.632
  pilA Haemophilus influenzae Rd KW20

61.111

100

0.611
  pilA Glaesserella parasuis strain SC1401

52.349

100

0.542
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

36.735

100

0.375
  pilA Vibrio cholerae strain A1552

36.735

100

0.375
  pilA Vibrio cholerae C6706

36.735

100

0.375

Multiple sequence alignment