Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   F2A31_RS14015 Genome accession   NZ_CP043909
Coordinates   3012156..3013868 (+) Length   570 a.a.
NCBI ID   WP_150027055.1    Uniprot ID   A0A5P1UXB4
Organism   Acinetobacter suaedae strain C16S1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3007156..3018868
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F2A31_RS13980 (F2A31_13980) nusA 3007960..3009444 (-) 1485 WP_150027047.1 transcription termination factor NusA -
  F2A31_RS13985 (F2A31_13985) rimP 3009482..3010006 (-) 525 WP_150027049.1 ribosome maturation factor RimP -
  F2A31_RS14005 (F2A31_14005) secG 3010642..3010971 (-) 330 WP_150027051.1 preprotein translocase subunit SecG -
  F2A31_RS14010 (F2A31_14010) tpiA 3010984..3011775 (-) 792 WP_150027053.1 triose-phosphate isomerase -
  F2A31_RS14015 (F2A31_14015) pilB 3012156..3013868 (+) 1713 WP_150027055.1 type IV-A pilus assembly ATPase PilB Machinery gene
  F2A31_RS14020 (F2A31_14020) pilC 3013893..3015119 (+) 1227 WP_150027058.1 type II secretion system F family protein Machinery gene
  F2A31_RS14025 (F2A31_14025) pilD 3015119..3015979 (+) 861 WP_150027060.1 A24 family peptidase Machinery gene
  F2A31_RS14030 (F2A31_14030) coaE 3015981..3016586 (+) 606 WP_005088002.1 dephospho-CoA kinase -
  F2A31_RS14035 (F2A31_14035) - 3016583..3017494 (-) 912 WP_150027062.1 DMT family transporter -
  F2A31_RS14045 (F2A31_14045) rlmB 3017535..3018281 (-) 747 WP_115736169.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  F2A31_RS14050 (F2A31_14050) - 3018391..3018717 (-) 327 WP_004637677.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63339.72 Da        Isoelectric Point: 5.0805

>NTDB_id=386484 F2A31_RS14015 WP_150027055.1 3012156..3013868(+) (pilB) [Acinetobacter suaedae strain C16S1]
MSGLHTSPKFSGFIRRLVEDGLMSASTMQTAIDTAKKTQQDIVAYLIEHHRLSQLTIAESIAVEFGEPIFDLSVFDTALL
PRELADSKLIQKHRVAPLIQRGQILYVATSNPTNIDALDAIRFNTKLNIEPVIVEHSKLEKLIEQQFGDTDSFDFGENED
FDIDLDVTPESQKDEDDTPQGEEAPIVKYINKLLIDAIRMGASDLHFEPYEKMYRVRYRVDGVLRQIANPPLQLATRLAS
RLKVMSQMDISEKRMPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIEALGYEDEQKALFLEALEKP
QGMLLITGPTGSGKTVSLYTGLNILNTEDTNISTAEDPVEINLEGINQVNVNTKTGLTFSAALKAFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSHCKTPIEIPKQSLLELGF
TEQDLANPELQIFQPVGCSECREGYKGRVGIYEVMKVTPEISKIIMEDGNALQIAAASEKAGFNNLRRSGLKKVMQGITS
LQEINRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=386484 F2A31_RS14015 WP_150027055.1 3012156..3013868(+) (pilB) [Acinetobacter suaedae strain C16S1]
ATGTCAGGATTACATACGTCTCCAAAATTTTCGGGGTTTATTCGACGGTTAGTCGAAGATGGTCTTATGTCTGCATCGAC
AATGCAAACTGCAATCGATACGGCAAAAAAAACACAACAAGATATTGTTGCCTATTTAATTGAACATCATCGCCTCTCAC
AGCTCACAATTGCTGAATCAATTGCGGTCGAGTTTGGTGAGCCTATTTTTGATTTATCTGTGTTTGATACGGCACTACTG
CCACGTGAACTTGCAGATAGCAAATTAATTCAAAAGCATCGTGTTGCCCCTCTCATTCAACGAGGGCAAATACTTTATGT
TGCAACAAGTAATCCCACCAACATTGATGCACTGGATGCCATTCGATTTAACACCAAGCTCAATATCGAGCCTGTTATTG
TAGAGCACAGTAAACTTGAAAAACTGATTGAGCAACAGTTTGGAGATACTGATAGTTTTGACTTCGGTGAAAATGAAGAT
TTTGATATTGACCTTGATGTCACCCCTGAGTCTCAAAAAGATGAGGATGACACACCTCAAGGCGAAGAGGCTCCAATTGT
AAAATATATCAATAAGCTGCTCATTGACGCGATTCGCATGGGTGCTTCTGATTTACATTTCGAACCCTATGAAAAAATGT
ATCGGGTTCGTTATCGCGTCGATGGTGTATTACGTCAAATCGCCAATCCGCCTTTACAGCTTGCGACACGCCTTGCTTCA
CGCTTAAAAGTAATGTCTCAAATGGACATCTCTGAAAAACGAATGCCACAAGATGGTCGTATTAAGTTAAAACTTTCTAA
AACTAAAGCAATTGATTTTCGTGTTAACTCTCTCCCAACACTTTTTGGTGAGAAACTTGTTTTACGTATTCTTGATCCTT
CCAGCGCCATGCTGGGAATTGAGGCACTCGGTTACGAAGATGAGCAAAAGGCTCTCTTCCTTGAAGCACTTGAAAAACCT
CAAGGCATGCTCTTAATCACAGGTCCAACAGGTTCAGGTAAAACAGTATCGTTATATACTGGTCTCAACATTCTGAATAC
CGAAGACACCAATATTTCTACTGCTGAAGATCCAGTGGAGATTAACTTAGAAGGCATCAATCAGGTTAACGTCAATACTA
AGACAGGCCTCACCTTCTCTGCCGCGCTGAAAGCATTCTTACGTCAAGATCCAGACATTATCATGGTCGGTGAGATTCGT
GACTTAGAAACGGCCGAGATTGCGATCAAAGCAGCTCAAACGGGTCACATGGTAATGTCCACATTGCATACCAACAGTGC
ACCTGAAACACTCACGCGTTTACGTAATATGGGCGTTCCCTCATTCAATATTGCAACGTCAGTCAATCTTGTGATTGCAC
AACGCTTAGCCCGTCGTTTATGCTCACACTGTAAAACCCCAATAGAAATTCCCAAACAAAGTCTGTTAGAACTCGGCTTT
ACTGAACAGGATTTAGCAAATCCTGAATTACAGATATTCCAACCTGTAGGATGTTCAGAGTGTCGTGAAGGTTATAAAGG
CCGTGTGGGTATTTATGAAGTGATGAAAGTCACGCCTGAGATCTCTAAGATTATTATGGAGGATGGCAATGCTTTACAAA
TCGCAGCAGCATCAGAAAAAGCAGGATTTAATAATTTAAGACGATCAGGTTTAAAAAAGGTCATGCAAGGCATCACCTCA
TTGCAAGAAATTAACCGCGTCACAAGTGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-149)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5P1UXB4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

82.662

100

0.828
  pilB Acinetobacter baylyi ADP1

77.233

100

0.774
  pilB Legionella pneumophila strain ERS1305867

54.034

93.509

0.505
  pilF Neisseria gonorrhoeae MS11

48.768

99.649

0.486
  pilB Vibrio parahaemolyticus RIMD 2210633

49.283

97.895

0.482
  pilB Vibrio cholerae strain A1552

49.275

96.842

0.477
  pilB Vibrio campbellii strain DS40M4

48.387

97.895

0.474
  pilF Thermus thermophilus HB27

37.5

98.246

0.368
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.171

93.158

0.365

Multiple sequence alignment