Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   F1C79_RS21545 Genome accession   NZ_CP043626
Coordinates   4666508..4668586 (-) Length   692 a.a.
NCBI ID   WP_081515421.1    Uniprot ID   A0A9X7N2R7
Organism   Pseudomonas denitrificans (nom. rej.) strain BG1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4661508..4673586
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F1C79_RS21530 (F1C79_21535) - 4663126..4664817 (-) 1692 WP_151188549.1 AAA family ATPase -
  F1C79_RS21535 (F1C79_21540) aroB 4664824..4665930 (-) 1107 WP_151188550.1 3-dehydroquinate synthase -
  F1C79_RS21540 (F1C79_21545) aroK 4665981..4666499 (-) 519 WP_081515781.1 shikimate kinase AroK -
  F1C79_RS21545 (F1C79_21550) pilQ 4666508..4668586 (-) 2079 WP_081515421.1 type IV pilus secretin PilQ Machinery gene
  F1C79_RS21550 (F1C79_21555) pilP 4668640..4669164 (-) 525 WP_081515420.1 type 4a pilus biogenesis lipoprotein PilP -
  F1C79_RS21555 (F1C79_21560) pilO 4669161..4669823 (-) 663 WP_081515419.1 type 4a pilus biogenesis protein PilO -
  F1C79_RS21560 (F1C79_21565) pilN 4669820..4670413 (-) 594 WP_081515418.1 type 4a pilus biogenesis protein PilN -
  F1C79_RS21565 (F1C79_21570) pilM 4670413..4671477 (-) 1065 WP_017520515.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 692 a.a.        Molecular weight: 74241.12 Da        Isoelectric Point: 5.1735

>NTDB_id=385536 F1C79_RS21545 WP_081515421.1 4666508..4668586(-) (pilQ) [Pseudomonas denitrificans (nom. rej.) strain BG1]
MNRKLSRLGISVLAAVFAPALLAANLQSVDVAALPGDRVELKLGFDEPVAAPRGYTIEQPARIALDLPGVQNKLGSKNRE
LGVGNARSLTVVEAKDRTRLIVNLATLVPYSTRAEGSNLYVVVGAGAATSSSYASAAPAAAPAARPAQPKPYVPAGKAIR
NIDFQRGEQGEGNVVIDLSDPTVSPDIQEQGGKIRLDFPRTQLPDNLRVRLDVKDFATPVQLVNASAVGDKASITIEPTG
LYDYLVYQTDNRMTVSIKPLTQDDAEKRKKDTFAYTGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITLRLQN
VPWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELEAQKQISELAPLRRELIQVNYAKAADIAKLFSSVTGSGA
SAPGVAAAAQGDNRGSITVDDRTNSIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYTKELGVNWSGSLSGH
GNFDVSGGTNSTGNTFVDLGTSNATPTSGISIGFTTNNTILDLQLNAMEKTGNGEVVSQPKVVTSDKETAKILKGSEIPY
QEASSSGATSVSFKEAALSLEVTPQITPDNRIIMEVKVNKDEPDYANALNGVPPIKKNEVNAKVLVNDGETIVIGGVFSN
TQTKSTAKVPFLGDVPMIGRLFKNDYVTDSKNELLVFLTPRIMNNQAIAIGR

Nucleotide


Download         Length: 2079 bp        

>NTDB_id=385536 F1C79_RS21545 WP_081515421.1 4666508..4668586(-) (pilQ) [Pseudomonas denitrificans (nom. rej.) strain BG1]
ATGAATAGAAAGCTTTCCCGCCTCGGCATTTCCGTGCTCGCGGCCGTATTCGCGCCGGCTCTGCTGGCCGCGAACCTGCA
GAGTGTCGACGTGGCGGCGCTGCCGGGCGATCGCGTGGAGCTGAAGCTGGGCTTCGATGAGCCGGTTGCCGCGCCGCGTG
GCTACACCATTGAGCAGCCGGCGCGCATCGCGCTGGACCTGCCGGGTGTGCAGAACAAGCTGGGCAGCAAGAACCGCGAG
CTGGGCGTGGGCAATGCCCGCAGCCTGACCGTGGTTGAGGCGAAGGACCGTACTCGCCTGATCGTCAATCTGGCGACCCT
CGTGCCCTACAGCACCCGTGCGGAAGGTTCGAACCTGTACGTCGTGGTCGGTGCGGGCGCAGCGACTTCCAGCTCCTACG
CCTCCGCGGCGCCGGCAGCGGCTCCAGCGGCCAGGCCCGCTCAGCCCAAGCCCTACGTCCCGGCTGGCAAGGCGATTCGT
AATATCGACTTCCAGCGTGGCGAGCAAGGCGAAGGCAATGTCGTGATCGATCTGTCCGATCCGACTGTCAGCCCGGATAT
TCAGGAGCAGGGCGGCAAGATCCGCCTGGACTTCCCGCGTACCCAGCTGCCGGACAATCTGCGCGTCCGCCTGGACGTGA
AGGACTTCGCGACCCCGGTACAGCTGGTCAACGCTTCCGCGGTCGGCGACAAGGCCAGCATCACCATCGAGCCGACCGGT
CTGTACGACTACCTCGTCTACCAGACCGACAATCGCATGACCGTCAGCATCAAACCGCTGACCCAGGACGATGCCGAGAA
GCGCAAGAAGGACACCTTCGCCTACACCGGTGAAAAGCTGTCGCTGAACTTCCAGGATATCGATGTGCGCTCGGTGCTGC
AGCTGATCGCCGACTTCACCGACCTGAACCTGGTGGCTTCGGACACCGTGCAGGGCAACATCACCCTTCGTCTGCAGAAC
GTGCCGTGGGATCAGGCGCTGGATCTGGTGCTCAAGACCAAGGGCCTGGACAAGCGCAAGGTCGGCAACGTATTGCTGGT
CGCACCGGCTGACGAGATCGCGGCTCGCGAGCGCCAGGAGCTGGAAGCGCAGAAGCAGATCTCCGAACTGGCTCCGCTGC
GTCGCGAGCTGATTCAGGTGAACTACGCCAAGGCGGCGGACATTGCCAAGCTGTTCAGCTCCGTCACTGGCAGCGGTGCC
AGCGCTCCGGGTGTTGCGGCGGCTGCACAGGGCGACAACCGCGGTTCGATCACCGTGGATGACCGCACCAACAGCATCAT
TGCCTACCAGACCCAGGATCGCCTGGATGAGCTGCGCCGCATCGTGGCCCAGCTGGACATCCCGGTTCGTCAGGTGATGA
TCGAGGCGCGCATCGTCGAAGCCAACGTCGACTACACCAAGGAACTGGGTGTGAACTGGAGCGGCTCGCTCTCCGGCCAC
GGCAACTTCGATGTCTCCGGCGGCACCAACAGCACTGGCAACACCTTCGTTGACCTGGGCACGTCGAACGCCACCCCGAC
TTCGGGCATCAGCATCGGCTTCACCACCAACAACACCATCCTGGATCTCCAGCTCAACGCCATGGAGAAGACCGGTAACG
GTGAAGTGGTGTCCCAGCCGAAGGTCGTCACCTCGGACAAGGAAACCGCGAAGATCCTGAAAGGCTCGGAGATTCCCTAC
CAGGAAGCCAGCTCCAGCGGCGCGACCTCGGTGTCCTTCAAGGAAGCGGCGCTGTCCCTGGAAGTGACCCCGCAGATCAC
TCCGGACAACCGCATCATCATGGAAGTGAAGGTCAACAAGGACGAGCCGGACTACGCCAACGCCCTGAACGGCGTACCGC
CGATCAAGAAGAACGAGGTGAACGCGAAAGTGCTGGTCAACGATGGCGAAACCATCGTGATTGGTGGCGTGTTCTCCAAC
ACCCAGACCAAGTCCACGGCCAAGGTGCCGTTCCTGGGCGACGTGCCGATGATCGGCCGCCTGTTCAAGAACGACTATGT
GACCGACAGCAAGAACGAGCTGCTGGTTTTCCTTACCCCGCGGATCATGAATAATCAGGCCATTGCAATCGGCCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(303-349)

Bacterial type II and III secretion system protein (Secretin)

(527-683)

Bacterial type II/III secretion system short domain (Secretin_N)

(377-451)

AMIN domain (AMIN)

(40-121)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

78.255

100

0.816
  comQ Acinetobacter baylyi ADP1

42.547

99.855

0.425
  pilQ Acinetobacter baumannii strain A118

39.592

100

0.421
  pilQ Acinetobacter baumannii D1279779

39.592

100

0.421
  pilQ Legionella pneumophila strain Lp02

39.706

98.266

0.39
  pilQ Legionella pneumophila strain ERS1305867

39.706

98.266

0.39

Multiple sequence alignment