Detailed information NTG0385537

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: F1C79_RS21565
• Genome accession: NZ_CP043626
• Coordinates: 4670413..4671477 (-)
• Length: 354 a.a.
• NCBI ID: WP_017520515.1
• Uniprot ID: A0A5R9R179
• Organism: Pseudomonas denitrificans (nom. rej.) strain BG1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4665413..4676477

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
F1C79_RS21540 (F1C79_21545)	aroK	4665981..4666499 (-)	519	WP_081515781.1	shikimate kinase AroK	-
F1C79_RS21545 (F1C79_21550)	pilQ	4666508..4668586 (-)	2079	WP_081515421.1	type IV pilus secretin PilQ	Machinery gene
F1C79_RS21550 (F1C79_21555)	pilP	4668640..4669164 (-)	525	WP_081515420.1	type 4a pilus biogenesis lipoprotein PilP	-
F1C79_RS21555 (F1C79_21560)	pilO	4669161..4669823 (-)	663	WP_081515419.1	type 4a pilus biogenesis protein PilO	-
F1C79_RS21560 (F1C79_21565)	pilN	4669820..4670413 (-)	594	WP_081515418.1	type 4a pilus biogenesis protein PilN	-
F1C79_RS21565 (F1C79_21570)	pilM	4670413..4671477 (-)	1065	WP_017520515.1	pilus assembly protein PilM	Machinery gene
F1C79_RS21570 (F1C79_21575)	-	4671664..4674092 (+)	2429	Protein_4298	penicillin-binding protein 1A	-
F1C79_RS21575 (F1C79_21580)	-	4674269..4674736 (+)	468	WP_151188551.1	hypothetical protein	-
F1C79_RS21580 (F1C79_21585)	-	4674730..4675224 (-)	495	WP_151188552.1	M48 family metallopeptidase	-
F1C79_RS21585 (F1C79_21590)	-	4675274..4676044 (-)	771	WP_151188553.1	class I SAM-dependent methyltransferase	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37747.01 Da
• Isoelectric Point: 4.4445

>NTDB_id=385537 F1C79_RS21565 WP_017520515.1 4670413..4671477(-) (pilM) [Pseudomonas denitrificans (nom. rej.) strain BG1]
MLGLFNKKANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIAELEGVGQALSRVVAKARTAVKTSAV
AVAGSAVITKTIEMDGGLSDDELENQLKIEADQYIPYPLEEVAIDFEVQGTSARNADRVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERAFSLLGDQLGGNADELTVAVVDIGATMTTLSVLHHGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLQPFKDAVVQQVSRSLQFFFAAGQFNDVDYILLAGGTASIPDLDRLIQQKIGTPTLVANP
FADMVLSGKVNAGALASDAPALMIACGLAMRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=385537 F1C79_RS21565 WP_017520515.1 4670413..4671477(-) (pilM) [Pseudomonas denitrificans (nom. rej.) strain BG1]
GTGCTAGGGCTCTTCAATAAGAAAGCGAACACGCTGCTGGGGATAGACATCAGTTCGACTTCGGTCAAACTTCTCGAATT
GAGCCGCTCTGGAGGCCGCTACAAGGTAGAGGCCTACGCTGTCGAGCCGCTCCCGCCCAATGCAGTGGTAGAAAAGAACA
TCGCTGAGCTGGAGGGTGTCGGCCAGGCGCTGTCACGTGTGGTCGCCAAGGCGCGGACCGCAGTGAAAACGTCTGCGGTC
GCGGTTGCCGGCTCGGCGGTCATCACTAAAACCATCGAGATGGACGGCGGACTCTCGGACGACGAGCTGGAGAATCAGCT
CAAGATCGAGGCCGACCAGTACATCCCCTATCCCCTCGAAGAGGTCGCGATCGACTTCGAGGTGCAGGGCACGTCCGCAC
GCAACGCGGATCGCGTCGATGTTCTTCTCGCCGCATGCCGGAAGGAGAACGTCGAGGTGCGCGAAGCTGCGCTGGCGCTC
GCGGGCCTGACGGCCAAGGTCGTCGACGTCGAAGCCTACGCGCTGGAGCGAGCGTTCAGTCTGCTGGGAGACCAGTTGGG
CGGCAATGCCGACGAGCTCACAGTTGCGGTGGTCGATATCGGCGCCACCATGACCACGCTGAGCGTCCTGCACCATGGCC
GCACCATCTATACGCGTGAGCAGCTTTTCGGCGGGCGTCAGCTCACCGAGGAGATCCAGCGCCGCTATGGACTGTCGGTC
GAGGAAGCGGGTCTCGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAAGTGTTGCAGCCGTTCAAGGACGC
CGTGGTTCAGCAGGTGTCGCGTTCGCTGCAGTTCTTCTTCGCGGCCGGCCAGTTCAACGATGTGGACTACATCCTGCTGG
CGGGAGGCACTGCGTCCATCCCTGACCTAGATCGTCTGATCCAGCAGAAGATCGGCACTCCGACCCTGGTGGCCAACCCC
TTCGCCGACATGGTGCTGAGTGGCAAGGTCAACGCCGGAGCGCTGGCCAGTGATGCGCCCGCGCTGATGATTGCCTGCGG
TCTGGCGATGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A5R9R179

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	57.345	100	0.573
comM	Acinetobacter nosocomialis M2	57.345	100	0.573
comM	Acinetobacter baylyi ADP1	53.39	100	0.534
pilM	Legionella pneumophila strain ERS1305867	46.61	100	0.466